Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC77_RS01695 Genome accession   NZ_CP027030
Coordinates   348708..350492 (+) Length   594 a.a.
NCBI ID   WP_011079356.1    Uniprot ID   A0A3Q0L3M5
Organism   Vibrio vulnificus strain FORC_077     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343708..355492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS01675 (FORC77_0306) pilM 346022..347029 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC77_RS01680 (FORC77_0307) pilN 347019..347609 (+) 591 WP_131069608.1 PilN domain-containing protein Machinery gene
  FORC77_RS01685 (FORC77_0308) pilO 347602..348192 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC77_RS01690 (FORC77_0309) pilP 348182..348694 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC77_RS01695 (FORC77_0310) pilQ 348708..350492 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ family protein Machinery gene
  FORC77_RS01700 (FORC77_0311) aroK 350694..351212 (+) 519 WP_011079357.1 shikimate kinase AroK -
  FORC77_RS01705 (FORC77_0312) aroB 351245..352336 (+) 1092 WP_061058951.1 3-dehydroquinate synthase -
  FORC77_RS01710 (FORC77_0313) - 352387..353883 (+) 1497 WP_061058950.1 AAA family ATPase -
  FORC77_RS01715 (FORC77_0314) - 353963..354790 (+) 828 WP_017789766.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64520.62 Da        Isoelectric Point: 4.5316

>NTDB_id=274579 FORC77_RS01695 WP_011079356.1 348708..350492(+) (pilQ) [Vibrio vulnificus strain FORC_077]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=274579 FORC77_RS01695 WP_011079356.1 348708..350492(+) (pilQ) [Vibrio vulnificus strain FORC_077]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTGGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGGTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGCAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATTATCAAGGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTGAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTGCGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTTGGTGGTTCAATCGAAAACAACCTGGCAACCATTGGTCTCTATAATG
CAGGCAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCGAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGTACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.729

99.327

0.732
  pilQ Vibrio cholerae strain A1552

73.729

99.327

0.732
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment