Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC77_RS01675 Genome accession   NZ_CP027030
Coordinates   346022..347029 (+) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus strain FORC_077     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341022..352029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS01660 (FORC77_0303) - 341853..342125 (-) 273 WP_011079349.1 DUF3624 domain-containing protein -
  FORC77_RS01665 (FORC77_0304) oxyR 342399..343298 (+) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  FORC77_RS01670 (FORC77_0305) - 343369..345882 (-) 2514 WP_131069607.1 PBP1A family penicillin-binding protein -
  FORC77_RS01675 (FORC77_0306) pilM 346022..347029 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC77_RS01680 (FORC77_0307) pilN 347019..347609 (+) 591 WP_131069608.1 PilN domain-containing protein Machinery gene
  FORC77_RS01685 (FORC77_0308) pilO 347602..348192 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC77_RS01690 (FORC77_0309) pilP 348182..348694 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC77_RS01695 (FORC77_0310) pilQ 348708..350492 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ family protein Machinery gene
  FORC77_RS01700 (FORC77_0311) aroK 350694..351212 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=274575 FORC77_RS01675 WP_017419656.1 346022..347029(+) (pilM) [Vibrio vulnificus strain FORC_077]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=274575 FORC77_RS01675 WP_017419656.1 346022..347029(+) (pilM) [Vibrio vulnificus strain FORC_077]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGTCATTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCAATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment