Detailed information NTG0273787

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: LL223_RS11900
• Genome accession: NZ_CP031926
• Coordinates: 2297097..2298035 (-)
• Length: 312 a.a.
• NCBI ID: WP_010906320.1
• Uniprot ID: Q9CDT8
• Organism: Lactococcus lactis subsp. lactis strain 223
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2292097..2303035

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL223_RS11865 (LL223_2271)	-	2292274..2292483 (-)	210	WP_023349177.1	helix-turn-helix transcriptional regulator	-
LL223_RS11870 (LL223_2272)	-	2292674..2293096 (+)	423	WP_023349178.1	helix-turn-helix transcriptional regulator	-
LL223_RS11875 (LL223_2273)	-	2293107..2293691 (+)	585	WP_101916262.1	hypothetical protein	-
LL223_RS11880 (LL223_2274)	-	2293745..2294407 (+)	663	WP_023349180.1	SHOCT domain-containing protein	-
LL223_RS11885 (LL223_2275)	-	2294522..2295979 (+)	1458	WP_058147724.1	recombinase family protein	-
LL223_RS11890 (LL223_03270)	-	2295976..2296116 (-)	141	WP_228777719.1	hypothetical protein	-
LL223_RS11895 (LL223_2276)	comGB	2296130..2297203 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL223_RS11900 (LL223_2277)	comGA	2297097..2298035 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 312 a.a.
• Molecular weight: 35719.89 Da
• Isoelectric Point: 6.1804

>NTDB_id=273787 LL223_RS11900 WP_010906320.1 2297097..2298035(-) (comGA) [Lactococcus lactis subsp. lactis strain 223]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide

• Length: 939 bp

>NTDB_id=273787 LL223_RS11900 WP_010906320.1 2297097..2298035(-) (comGA) [Lactococcus lactis subsp. lactis strain 223]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

3D structure

• AlphaFold DB: Q9CDT8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	76.603	100	0.766
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	54.662	99.679	0.545
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.055	99.679	0.529
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.09	99.679	0.519
comGA	Streptococcus gordonii str. Challis substr. CH1	51.923	100	0.519
comGA	Streptococcus mutans UA140	51.923	100	0.519
comGA	Streptococcus mutans UA159	51.923	100	0.519