Detailed information NTG0271427

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: C4N11_RS04560
• Genome accession: NZ_CP026670
• Coordinates: 853828..854478 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387328.1
• Uniprot ID: A0A4N9N7Z7
• Organism: Streptococcus pneumoniae strain endophthalmitis isolate 335
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 848828..859478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4N11_RS04515 (C4N11_04515)	-	849311..849526 (+)	216	WP_001232085.1	YozE family protein	-
C4N11_RS04520 (C4N11_04520)	-	849612..850580 (+)	969	WP_000658183.1	PhoH family protein	-
C4N11_RS04525 (C4N11_04525)	-	850558..850737 (-)	180	WP_001813350.1	hypothetical protein	-
C4N11_RS04530 (C4N11_04530)	-	850773..851274 (+)	502	Protein_850	GNAT family N-acetyltransferase	-
C4N11_RS04535 (C4N11_04535)	-	851277..851603 (+)	327	Protein_851	TfoX/Sxy family protein	-
C4N11_RS12555	ald	851904..853014 (-)	1111	Protein_852	alanine dehydrogenase	-
C4N11_RS04555 (C4N11_04555)	-	853191..853760 (+)	570	WP_000443777.1	GNAT family N-acetyltransferase	-
C4N11_RS04560 (C4N11_04560)	comEA/celA/cilE	853828..854478 (+)	651	WP_000387328.1	ComEA family DNA-binding protein	Machinery gene
C4N11_RS04565 (C4N11_04565)	comEC/celB	854462..856702 (+)	2241	WP_000942392.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C4N11_RS12560 (C4N11_04570)	-	856844..857068 (+)	225	WP_000583432.1	hypothetical protein	-
C4N11_RS04575 (C4N11_04575)	-	857101..857688 (+)	588	WP_000939884.1	ATP-binding cassette domain-containing protein	-
C4N11_RS04580 (C4N11_04580)	-	857692..858873 (+)	1182	WP_000655951.1	membrane protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23230.59 Da
• Isoelectric Point: 5.0667

>NTDB_id=271427 C4N11_RS04560 WP_000387328.1 853828..854478(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain endophthalmitis isolate 335]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=271427 C4N11_RS04560 WP_000387328.1 853828..854478(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain endophthalmitis isolate 335]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAGAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A4N9N7Z7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae D39	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae R6	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis SK321	89.815	100	0.898
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366