Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C3F22_RS00035 Genome accession   NZ_CP026420
Coordinates   4749..6458 (-) Length   569 a.a.
NCBI ID   WP_104425602.1    Uniprot ID   A0A2L0I1I5
Organism   Acinetobacter sp. ACNIH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..11458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F22_RS00010 (C3F22_00010) rlmB 273..1022 (+) 750 WP_104425597.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  C3F22_RS00015 (C3F22_00015) - 1066..1983 (+) 918 WP_104425598.1 DMT family transporter -
  C3F22_RS00020 (C3F22_00020) coaE 2022..2627 (-) 606 WP_104425599.1 dephospho-CoA kinase -
  C3F22_RS00025 (C3F22_00025) pilD 2629..3489 (-) 861 WP_104425600.1 prepilin peptidase Machinery gene
  C3F22_RS00030 (C3F22_00030) pilC 3489..4718 (-) 1230 WP_104425601.1 type II secretion system F family protein Machinery gene
  C3F22_RS00035 (C3F22_00035) pilB 4749..6458 (-) 1710 WP_104425602.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C3F22_RS00040 (C3F22_00040) tpiA 6738..7529 (+) 792 WP_104425603.1 triose-phosphate isomerase -
  C3F22_RS00045 (C3F22_00045) secG 7543..7872 (+) 330 WP_004787204.1 preprotein translocase subunit SecG -
  C3F22_RS00075 (C3F22_00075) rimP 8837..9361 (+) 525 WP_034169753.1 ribosome maturation factor RimP -
  C3F22_RS00080 (C3F22_00080) nusA 9397..10881 (+) 1485 WP_004787201.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63438.46 Da        Isoelectric Point: 4.7970

>NTDB_id=269209 C3F22_RS00035 WP_104425602.1 4749..6458(-) (pilB) [Acinetobacter sp. ACNIH1]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNSNVLYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDVDTTDPNKEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
EADLQSPEFQIYEPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=269209 C3F22_RS00035 WP_104425602.1 4749..6458(-) (pilB) [Acinetobacter sp. ACNIH1]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTGGTCGATGAAGGGGTATTATCGGCTGAAGA
TATGCGTACTGCTTTAGCCAATGCCAAACAAGAAAAAACGGATATCGTGGCCTATTTGGTCAGTCATCATAATCTTTCCC
CTACCACCATTGCCGAAACGATCTCAGTAGAATTTGGTGAGCCTTTATTTGATATTGGCGCTTATGATCCAGGACAAATC
CTGCGTGAAGATATCGATGAAAAGCTAATTACCAAACATCGAATTTTGCCAATTTATAAGAATTCCAATGTTCTTTATGT
TGCCACGAGCAATCCAACCAATATTGAAGCAACGGATGCCATCCGCTTTGCAACCAAACTGAATATCGAAACTGTTATTG
TTGAACACAATAAACTTGAAAAACTGATTGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTTGATGATGATTTCGAT
CTGGATGTCGATGTCGACACAACCGATCCAAATAAAGAAGAAGACGAAAAAGACAAAGGTGAAGAAGCCCCGATTGTTAA
ATATATTAATAAATTACTGATTGATGCCATTCGTATGGGGGCATCAGATTTACATTTTGAACCTTATGAAAAAATGTATC
GGGTACGCTATCGTGTCGATGGCGTTTTACGGCAGATTGCGACACCGCCGCTACAGCTAGCAAACCGTTTAGCGTCACGT
TTAAAAGTTATGTCGCAGATGGACATTTCTGAGAAACGTGTTCCTCAAGACGGCCGTATTAAATTAAAGCTCTCAAAAAA
CAAAGCTATCGACTTCCGTGTTAATTCACTCCCTACCCTGTTTGGTGAAAAACTGGTTCTACGTATTCTAGATCCATCCA
GTGCCATGTTGGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAACTATTTATGGAAGCCCTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACGGGCTTAAACATCTTAAACCGCGA
GGATACCAATATTTCTACTGCGGAAGATCCAGTCGAGATTAATCTGCAAGGGATTAATCAGGTTAACGTGAATAATAAAG
TTGGCCTGACTTTCGCAGCGGCATTAAAATCCTTCTTGCGTCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAT
CTGGAAACAGCCGAAATTGCAATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGTGCTCC
GGAAACTCTGACCCGTTTACGTAATATGGGCGTCCCTTCTTTCAATATCGCGACCTCGGTCAATCTAGTGATTGCGCAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGTAAAAAGCCGGCTGATATTCCTCAGCAAAGTTTGCTAGAAATGGGCTTTACC
GAAGCAGATTTACAAAGCCCTGAATTTCAGATTTATGAGCCAGTCGGCTGTAGTGAATGCCGTGAAGGTTATAAGGGCCG
GGTCGGTATTTACGAAGTCATGAAAGTCACACCTGAAATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCGCGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0I1I5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.81
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilF Neisseria gonorrhoeae MS11

49.198

98.594

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.198

98.594

0.485
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilB Vibrio cholerae strain A1552

50.385

91.388

0.46
  pilF Thermus thermophilus HB27

38.272

99.649

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.374

Multiple sequence alignment