Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C3F34_RS05100 Genome accession   NZ_CP026412
Coordinates   1092533..1094239 (+) Length   568 a.a.
NCBI ID   WP_104441192.1    Uniprot ID   A0A2L0HTA8
Organism   Acinetobacter sp. ACNIH2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1087533..1099239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F34_RS05060 (C3F34_05060) nusA 1088292..1089776 (-) 1485 WP_016168395.1 transcription termination factor NusA -
  C3F34_RS05065 (C3F34_05065) rimP 1089812..1090336 (-) 525 WP_104441190.1 ribosome maturation factor RimP -
  C3F34_RS05090 (C3F34_05090) secG 1091118..1091447 (-) 330 WP_016168397.1 preprotein translocase subunit SecG -
  C3F34_RS05095 (C3F34_05095) tpiA 1091461..1092252 (-) 792 WP_104441191.1 triose-phosphate isomerase -
  C3F34_RS05100 (C3F34_05100) pilB 1092533..1094239 (+) 1707 WP_104441192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C3F34_RS05105 (C3F34_05105) pilC 1094268..1095494 (+) 1227 WP_044739185.1 type II secretion system F family protein Machinery gene
  C3F34_RS20695 (C3F34_05110) pilD 1095494..1096354 (+) 861 WP_044739184.1 prepilin peptidase Machinery gene
  C3F34_RS05115 (C3F34_05115) coaE 1096357..1096974 (+) 618 WP_044739183.1 dephospho-CoA kinase -
  C3F34_RS05120 (C3F34_05120) - 1096971..1097888 (-) 918 WP_044739182.1 DMT family transporter -
  C3F34_RS05125 (C3F34_05125) rlmB 1097922..1098674 (-) 753 WP_044739181.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  C3F34_RS05130 (C3F34_05130) - 1098811..1099134 (-) 324 WP_016168405.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63636.09 Da        Isoelectric Point: 5.0425

>NTDB_id=269170 C3F34_RS05100 WP_104441192.1 1092533..1094239(+) (pilB) [Acinetobacter sp. ACNIH2]
MTALQGSPRFTGFIRRLVEDGIVSTENMQAAVNSARKTNQDIVPYLIENLKLKPIVIAEKISEEFGEPLFDLSTYDSAHI
LRDVVEDKLITKFRVLPIFRRGHQLFVATSNPTHMDAMDAMRFNSKLSIEAIIVEHDKLEKIIEQNYAEESTFEFDDDFE
LHVHEEPTDQEDNDEQPQEDEAPIVKYINKLLIDAIRMGASDLHFEPYEKVYRVRYRVDGVLRQIATPPLQLANRLASRL
KVMSQMDIAEKRVPQDGRIKLKISKSKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFMEALEKPQG
MLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPAEIPKNSLLEMGFTE
QEIEQPDFKIFQPVGCSECRDGYKGRVGIYEVMKVTPEISKIIMEDGNAIQIAEASAKLGFNNLRISGLKKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=269170 C3F34_RS05100 WP_104441192.1 1092533..1094239(+) (pilB) [Acinetobacter sp. ACNIH2]
ATGACAGCACTACAAGGTTCTCCACGATTTACAGGTTTTATTCGCCGCTTGGTCGAAGATGGGATTGTTTCTACTGAAAA
CATGCAGGCTGCAGTTAACAGCGCAAGAAAAACAAACCAAGATATTGTCCCTTATCTAATTGAAAACCTAAAACTCAAAC
CGATTGTCATTGCCGAAAAAATTTCAGAAGAATTTGGTGAACCCTTATTTGATCTTTCGACTTATGACTCTGCCCATATT
TTAAGAGATGTCGTTGAAGACAAATTGATTACTAAATTCCGCGTGTTGCCGATTTTCCGACGTGGACATCAACTCTTTGT
AGCCACCAGCAATCCAACGCATATGGATGCCATGGATGCTATGCGCTTCAACAGCAAACTCAGTATTGAAGCGATTATTG
TTGAACATGACAAATTAGAAAAAATTATTGAACAAAATTATGCAGAAGAAAGCACTTTCGAGTTTGATGATGACTTTGAA
TTGCACGTCCATGAAGAGCCTACGGATCAAGAAGACAATGATGAGCAACCTCAAGAAGATGAAGCTCCAATTGTCAAATA
TATCAATAAATTATTGATTGATGCGATTCGTATGGGCGCATCCGACTTACATTTTGAACCCTATGAAAAAGTCTATCGTG
TGCGCTATCGTGTCGATGGGGTTTTACGCCAGATTGCAACTCCTCCTCTACAGTTAGCCAACCGTCTAGCTTCACGCTTA
AAAGTCATGTCACAAATGGACATCGCTGAAAAACGTGTGCCACAAGATGGTCGTATTAAGCTAAAAATTTCAAAATCCAA
AGCGATTGATTTTCGTGTCAACTCACTACCAACCCTATTTGGGGAAAAAATTGTACTGCGTATTTTAGACCCTTCGAGTG
CAATGCTAGGAATTGATGCACTAGGTTATGAACCTGAACAAAAAGCCTTATTTATGGAAGCACTGGAAAAGCCACAAGGG
ATGCTACTTATCACAGGCCCGACTGGTTCTGGTAAAACGGTTTCTCTCTATACAGGCTTAAATATCCTGAACAAAGAAGA
TACCAATATTTCAACCGCAGAAGATCCTGTCGAAATTAACTTAGAAGGGATTAATCAGGTTAACGTTAACAATAAAGTTG
GCTTGACGTTCTCTGCTGCATTAAAGTCTTTCTTACGTCAAGATCCAGACATTGTTATGGTCGGTGAGATTCGAGATTTA
GAAACGGCTGAAATTGCGATTAAAGCCGCGCAAACAGGTCATATGGTGATGTCTACCCTACATACCAACAGCGCACCAGA
AACATTGACTCGTTTAAGAAATATGGGTGTCCCTTCCTTTAACATTGCAACCTCTGTAAACTTAGTCATTGCGCAACGAC
TCGCTCGTCGACTCTGTTCACAATGTAAAGTACCTGCTGAAATACCAAAAAATAGCTTGTTAGAAATGGGCTTCACTGAG
CAAGAGATCGAACAACCAGACTTTAAAATTTTTCAGCCTGTCGGTTGTTCAGAATGTCGAGACGGCTATAAAGGGCGTGT
GGGGATCTATGAAGTAATGAAAGTTACACCTGAAATATCCAAGATTATTATGGAAGATGGCAATGCAATTCAAATTGCAG
AAGCATCGGCAAAATTGGGGTTTAATAATCTACGTATTTCAGGTTTAAAGAAGGTCATGCAAGGGGTAACTTCACTACAA
GAAGTCAATCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-151)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0HTA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.175

100

0.805
  pilB Acinetobacter baylyi ADP1

76.316

100

0.766
  pilB Legionella pneumophila strain ERS1305867

52.533

93.838

0.493
  pilF Neisseria gonorrhoeae MS11

49.821

98.592

0.491
  pilB Vibrio cholerae strain A1552

48.404

99.296

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

51.838

91.021

0.472
  pilB Vibrio campbellii strain DS40M4

51.064

91.021

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

33.819

100

0.368
  pilF Thermus thermophilus HB27

36.814

99.472

0.366

Multiple sequence alignment