Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AHA_RS19550 Genome accession   NC_008570
Coordinates   4298377..4300092 (+) Length   571 a.a.
NCBI ID   WP_011707569.1    Uniprot ID   A0KPV7
Organism   Aeromonas hydrophila subsp. hydrophila ATCC 7966     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4293377..4305092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AHA_RS19525 (AHA_3864) pdhR 4294485..4295249 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AHA_RS19530 (AHA_3865) ampD 4295525..4296097 (-) 573 WP_011707565.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AHA_RS19535 (AHA_3866) - 4296245..4296715 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  AHA_RS19540 (AHA_3867) nadC 4296721..4297578 (+) 858 WP_011707567.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AHA_RS19545 (AHA_3868) tapA 4297901..4298383 (+) 483 WP_011707568.1 type IVa pilus major pilin TapA -
  AHA_RS19550 (AHA_3869) pilB 4298377..4300092 (+) 1716 WP_011707569.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AHA_RS19555 (AHA_3870) pilC 4300208..4301449 (+) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  AHA_RS19560 (AHA_3871) pilD 4301472..4302344 (+) 873 WP_011707571.1 A24 family peptidase Machinery gene
  AHA_RS19565 (AHA_3872) coaE 4302364..4302978 (+) 615 WP_011707572.1 dephospho-CoA kinase -
  AHA_RS19570 (AHA_3873) zapD 4303016..4303738 (+) 723 WP_024945431.1 cell division protein ZapD -
  AHA_RS19575 (AHA_3874) yacG 4303748..4303942 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  AHA_RS19580 (AHA_3875) mutT 4304003..4304416 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62901.41 Da        Isoelectric Point: 5.4363

>NTDB_id=26832 AHA_RS19550 WP_011707569.1 4298377..4300092(+) (pilB) [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MLTMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAISKLMESDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDERQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=26832 AHA_RS19550 WP_011707569.1 4298377..4300092(+) (pilB) [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
GTGCTAACGATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGAAATCCTAGACAGCA
AGGCACTGGCTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGTTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAAAAGCATCACGTCCTGCCCATCTACACCCAAGGGCACACCCTCTATATCGC
TATGTCAGACCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTACATACCGAGGCGCTGCTGGTCG
AGGAAAACAAACTCACCACTGCCATCAGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAA
TCCGAGATCTCCGAGCTGGAAGTGTCTGACGAGGGATCACGGCTCGACGAGAGCGTCAACACTGCTGACGATGACGCGCC
CATCGTCAAATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGA
CCAAATACCGCATCCGCTTTCGAATAGACGGTATTCTGCATGAGATAGCGACCCCACCGGTCAACCTAGCCAACCGCTTC
TCGGCTCGCCTCAAGGTGATGGCACGGCTCGATATCGCTGAGCGGCGACTGCCACAGGATGGCCGCATCAAGCTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCG
ACTCCTCCGCCGCACGCCTCAATATCGAGCAACTCGGTTTCGACGAGCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCG
AAGCCTCAGGGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCA
ATCCCAAGGCAGGGCTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAA
ATCCGGGATCTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTACACACCAA
CTCGGCGGCAGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTCGTACCCGAGGCTGAGCTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAA
GGGCCGGGTCGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAAGGGGCCAACTCTCTGC
AGATTGCCGCCATAGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACC
AGTCTCGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KPV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.497

100

0.557
  pilB Legionella pneumophila strain ERS1305867

54.336

98.949

0.538
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.424

0.508
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371

Multiple sequence alignment