Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1N51_RS10150 Genome accession   NZ_CP026315
Coordinates   2086954..2088639 (-) Length   561 a.a.
NCBI ID   WP_103410543.1    Uniprot ID   -
Organism   Vibrio campbellii strain BoB-90     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2081954..2093639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1N51_RS10120 (C1N51_10120) rplS 2082394..2082747 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  C1N51_RS10125 (C1N51_10125) yacG 2083114..2083308 (-) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  C1N51_RS10130 (C1N51_10130) zapD 2083371..2084111 (-) 741 WP_012128881.1 cell division protein ZapD -
  C1N51_RS10135 (C1N51_10135) coaE 2084139..2084753 (-) 615 WP_045451194.1 dephospho-CoA kinase -
  C1N51_RS10140 (C1N51_10140) pilD 2084754..2085623 (-) 870 WP_050910736.1 prepilin peptidase Machinery gene
  C1N51_RS10145 (C1N51_10145) pilC 2085696..2086919 (-) 1224 WP_050905227.1 type II secretion system F family protein Machinery gene
  C1N51_RS10150 (C1N51_10150) pilB 2086954..2088639 (-) 1686 WP_103410543.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1N51_RS10155 (C1N51_10155) - 2088640..2089125 (-) 486 WP_050905229.1 pilin -
  C1N51_RS10165 (C1N51_10165) nadC 2089391..2090278 (-) 888 WP_103410545.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1N51_RS10170 (C1N51_10170) ampD 2090371..2090922 (+) 552 WP_103410546.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1N51_RS10175 (C1N51_10175) pdhR 2091327..2092094 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62142.88 Da        Isoelectric Point: 5.1851

>NTDB_id=268094 C1N51_RS10150 WP_103410543.1 2086954..2088639(-) (pilB) [Vibrio campbellii strain BoB-90]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDISEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLNALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQFQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=268094 C1N51_RS10150 WP_103410543.1 2086954..2088639(-) (pilB) [Vibrio campbellii strain BoB-90]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCGGGAATACTGACCTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACAGAACACCTAA
GCTCTGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAGACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTACCGCTTAACCGCACCTCTTCAACTTTGATTCTCGCGGTAGCAGATCCAACCAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGTTTACAAGTCGAGCTTGTCCTAGCTGACTTTCGCGAGCTGACAGCAG
CCATTCGGCGTTTGTATGGTCGATCGCTTGGGCAAGAAAAATCAGGTCTTAAAGAAATCAACCAAGATGAACTAGCGGGT
TTAGTTGATATTAGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTCTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCTTACGAAAATATGTACCGCGTACGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAACCTCCAAGTCATTTAAGCCGTCGTCTCTCCGCTCGTATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGCTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGGGAAAAGATTGTTCTGCGCTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGTTATAGCGACTCACAGAAACAACTTTATTTGAATGCCCTGCGCCGTCCACAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAGACGGTCTCGCTCTACACTGGATTGAACATTCTTAATAAACCAGAGATCAA
TATCTCAACCGCAGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTCCGCTCTTTCTTACGTCAAGACCCTGATGTAGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTGCACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCCTCTCTCAGTTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAATTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCCTCGGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.396

100

0.984
  pilB Vibrio parahaemolyticus RIMD 2210633

90.374

100

0.904
  pilB Vibrio cholerae strain A1552

73.665

100

0.738
  pilB Acinetobacter baylyi ADP1

47.935

99.287

0.476
  pilB Acinetobacter baumannii D1279779

50.864

92.87

0.472
  pilB Legionella pneumophila strain ERS1305867

48.524

96.613

0.469
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

36.364

100

0.385
  pilF Thermus thermophilus HB27

36.655

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

99.465

0.362

Multiple sequence alignment