Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C2U47_RS07390 Genome accession   NZ_CP026226
Coordinates   1580152..1581858 (+) Length   568 a.a.
NCBI ID   WP_103261554.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1575152..1586858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U47_RS07360 (C2U47_07360) pdhR 1576380..1577144 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  C2U47_RS07370 (C2U47_07370) ampD 1577421..1577993 (-) 573 WP_103261551.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C2U47_RS07375 (C2U47_07375) - 1578122..1578592 (+) 471 WP_042015101.1 retropepsin-like aspartic protease -
  C2U47_RS07380 (C2U47_07380) nadC 1578596..1579459 (+) 864 WP_103261552.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C2U47_RS23620 (C2U47_07385) - 1579770..1580144 (+) 375 WP_103261553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C2U47_RS07390 (C2U47_07390) pilB 1580152..1581858 (+) 1707 WP_103261554.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C2U47_RS07395 (C2U47_07395) pilC 1581986..1583227 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  C2U47_RS07400 (C2U47_07400) pilD 1583305..1584180 (+) 876 WP_103261555.1 A24 family peptidase Machinery gene
  C2U47_RS07405 (C2U47_07405) coaE 1584199..1584813 (+) 615 WP_043154105.1 dephospho-CoA kinase -
  C2U47_RS07410 (C2U47_07410) zapD 1584851..1585573 (+) 723 WP_010675825.1 cell division protein ZapD -
  C2U47_RS07415 (C2U47_07415) yacG 1585584..1585778 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  C2U47_RS07420 (C2U47_07420) mutT 1585836..1586240 (-) 405 WP_174218568.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62613.03 Da        Isoelectric Point: 5.8211

>NTDB_id=267913 C2U47_RS07390 WP_103261554.1 1580152..1581858(+) (pilB) [Aeromonas sp. ASNIH7]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSCLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=267913 C2U47_RS07390 WP_103261554.1 1580152..1581858(+) (pilB) [Aeromonas sp. ASNIH7]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAATGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATTTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGCTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTCACCGGTCCCACGGGGTCTGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTGAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGTGTCAACCAGGTGCAGATCAATCCCAAAG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCTGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37

Multiple sequence alignment