Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C2U40_RS19835 Genome accession   NZ_CP026217
Coordinates   4246890..4248596 (+) Length   568 a.a.
NCBI ID   WP_103827109.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4241890..4253596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U40_RS19805 (C2U40_19810) pdhR 4243038..4243802 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  C2U40_RS19810 (C2U40_19815) ampD 4244077..4244649 (-) 573 WP_103827107.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C2U40_RS19815 (C2U40_19820) - 4244796..4245266 (+) 471 WP_024944772.1 retropepsin-like aspartic protease -
  C2U40_RS19820 (C2U40_19825) nadC 4245272..4246129 (+) 858 WP_103827108.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C2U40_RS19830 (C2U40_19835) - 4246452..4246883 (+) 432 WP_041216993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C2U40_RS19835 (C2U40_19840) pilB 4246890..4248596 (+) 1707 WP_103827109.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C2U40_RS19840 (C2U40_19845) pilC 4248712..4249953 (+) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  C2U40_RS19845 (C2U40_19850) pilD 4249976..4250848 (+) 873 WP_103827110.1 A24 family peptidase Machinery gene
  C2U40_RS19850 (C2U40_19855) coaE 4250867..4251481 (+) 615 WP_103827111.1 dephospho-CoA kinase -
  C2U40_RS19855 (C2U40_19860) zapD 4251519..4252241 (+) 723 WP_024945431.1 cell division protein ZapD -
  C2U40_RS19860 (C2U40_19865) yacG 4252251..4252445 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  C2U40_RS19865 (C2U40_19870) mutT 4252506..4252919 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62539.95 Da        Isoelectric Point: 5.4342

>NTDB_id=267870 C2U40_RS19835 WP_103827109.1 4246890..4248596(+) (pilB) [Aeromonas sp. ASNIH4]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKVQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=267870 C2U40_RS19835 WP_103827109.1 4246890..4248596(+) (pilB) [Aeromonas sp. ASNIH4]
ATGACCTCCAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCAGA
TCCTACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACTACAGCCATCGGCAAGCTGATGGAGAGCGATCAAGATGCCCTTGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAAGTATCTGACGAGGGATCACGGCTCGACGAAAGCGTCAACACTGCTGACGATGACGCGCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCCTGCATGAGATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCTGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGCCAGAAGGTGCAATATCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTAGGTGAAATCCGGGAT
CTGGAAACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTATGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCTGGCCTCGAGAAGGCTCGCCTGGGTGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment