Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C2U30_RS16960 Genome accession   NZ_CP026122
Coordinates   3601822..3603528 (-) Length   568 a.a.
NCBI ID   WP_041211787.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596822..3608528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U30_RS16930 (C2U30_16935) mutT 3597440..3597844 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  C2U30_RS16935 (C2U30_16940) yacG 3597902..3598096 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  C2U30_RS16940 (C2U30_16945) zapD 3598107..3598829 (-) 723 WP_010675825.1 cell division protein ZapD -
  C2U30_RS16945 (C2U30_16950) coaE 3598867..3599481 (-) 615 WP_103244156.1 dephospho-CoA kinase -
  C2U30_RS16950 (C2U30_16955) pilD 3599500..3600375 (-) 876 WP_103244157.1 A24 family peptidase Machinery gene
  C2U30_RS16955 (C2U30_16960) pilC 3600453..3601694 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  C2U30_RS16960 (C2U30_16965) pilB 3601822..3603528 (-) 1707 WP_041211787.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C2U30_RS23110 (C2U30_16970) - 3603528..3603917 (-) 390 WP_080989820.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C2U30_RS16975 (C2U30_16980) nadC 3604230..3605093 (-) 864 WP_103244158.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C2U30_RS16980 (C2U30_16985) - 3605097..3605567 (-) 471 WP_042015101.1 retropepsin-like aspartic protease -
  C2U30_RS16985 (C2U30_16990) ampD 3605696..3606268 (+) 573 WP_103244159.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C2U30_RS16995 (C2U30_17000) pdhR 3606545..3607309 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62594.96 Da        Isoelectric Point: 5.8214

>NTDB_id=267331 C2U30_RS16960 WP_041211787.1 3601822..3603528(-) (pilB) [Aeromonas sp. ASNIH5]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=267331 C2U30_RS16960 WP_041211787.1 3601822..3603528(-) (pilB) [Aeromonas sp. ASNIH5]
ATGACCTCCTCACTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGTTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAACGATATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCACAGGATGGCCGGATCAAACTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATCGCTATCAAGGCCGCCCAGACCGGTCATCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAGGAAGGGATGCGCACCCTGCGCACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37

Multiple sequence alignment