Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C2H86_RS10325 Genome accession   NZ_CP026115
Coordinates   2269531..2270799 (-) Length   422 a.a.
NCBI ID   WP_159412477.1    Uniprot ID   -
Organism   Pseudomonas putida strain W5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2264531..2275799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H86_RS10310 (C2H86_24975) - 2266181..2267800 (-) 1620 WP_159412475.1 AAA family ATPase -
  C2H86_RS10315 (C2H86_24980) aroB 2267816..2268913 (-) 1098 WP_159412476.1 3-dehydroquinate synthase -
  C2H86_RS10320 (C2H86_24985) aroK 2269008..2269526 (-) 519 WP_060509908.1 shikimate kinase AroK -
  C2H86_RS10325 (C2H86_24990) pilQ 2269531..2270799 (-) 1269 WP_159412477.1 type IV pilus secretin PilQ family protein Machinery gene
  C2H86_RS10330 (C2H86_24995) - 2270723..2271739 (-) 1017 WP_240349699.1 pilus assembly protein PilP -
  C2H86_RS10335 (C2H86_25000) - 2271736..2272272 (-) 537 WP_159412478.1 PilN domain-containing protein -
  C2H86_RS10340 (C2H86_25005) pilM 2272272..2273123 (-) 852 WP_159412479.1 pilus assembly protein PilM -
  C2H86_RS10345 (C2H86_25010) - 2273300..2275753 (+) 2454 WP_159412480.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46037.87 Da        Isoelectric Point: 5.0124

>NTDB_id=267212 C2H86_RS10325 WP_159412477.1 2269531..2270799(-) (pilQ) [Pseudomonas putida strain W5]
MKWLLKWVVVALIPSQWCIAAPYRGEPISLNFQDVEVRSVLQVLADYAGINLVASDTVQGNVTLRLQEVPWDQALELVLR
SKGLARREEGNVLLVAPAAELAEQSRGTRIGQELDAQLQPLRRELLPIHHAKASDLAQLLLATLADDGILTGRGSLSVDA
RTNTLVAHQPADRLAELRQLVAQLDVPVQQVEIEARIVEANVDYEKGLGVRWGRQLYGEAPQLGKDLFVDLGMERGGAGI
GVGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=267212 C2H86_RS10325 WP_159412477.1 2269531..2270799(-) (pilQ) [Pseudomonas putida strain W5]
ATGAAATGGCTGTTGAAGTGGGTGGTGGTGGCGTTGATACCCAGTCAGTGGTGCATCGCGGCGCCCTATCGGGGTGAGCC
CATCTCGCTGAACTTTCAGGATGTCGAGGTGCGTTCGGTGCTGCAGGTGCTGGCTGACTACGCTGGGATCAACCTGGTGG
CCAGTGACACGGTGCAAGGTAATGTCACCCTGCGGTTGCAGGAGGTTCCGTGGGATCAGGCGCTGGAGCTGGTCCTTCGT
AGCAAGGGGCTGGCTCGGCGCGAGGAAGGGAATGTGCTGCTGGTGGCGCCAGCTGCCGAGTTGGCCGAGCAATCCCGCGG
CACCCGTATCGGGCAAGAGCTGGATGCGCAGTTGCAACCCTTGCGGCGTGAGTTGCTGCCGATCCATCACGCCAAGGCTT
CCGACCTTGCGCAGCTGCTGCTGGCAACCTTGGCGGATGATGGCATTCTGACCGGTCGTGGCAGCCTGAGCGTCGACGCT
CGCACCAACACCTTGGTCGCGCACCAGCCTGCTGACCGCCTTGCCGAGTTGCGGCAGTTGGTCGCTCAGCTTGACGTGCC
CGTACAGCAGGTAGAGATCGAGGCGCGCATCGTCGAAGCCAATGTCGACTATGAGAAAGGCTTGGGCGTGCGCTGGGGGC
GACAGCTGTATGGGGAAGCGCCACAGTTGGGGAAGGATCTGTTTGTCGACCTGGGCATGGAGCGGGGCGGCGCCGGCATT
GGTGTTGGCCTGCTGCGTGGCGGCGTGCTGCTGGATCTGGAGCTCAGCGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCCCAGCCCAAGGTGGTCACGGCTGACAAGGAAACTGCCAGGATTCTCAAGGGTACCGAGGTGCCTTATCAGGAAACCA
GCAAGAGTGGCGCCACCTCGGTGGCCTTTCGCGAGGCCTCGCTGTCGCTGGAGGTCACGCCACAGATCACCCCGGACGGG
AAGGTGATCATGACGGTCAAGGTGACCAAGGACGAGCCTGACTTCGTGAATGCCTTGAACGACGTGCCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTCCGTGTGGCGGACGGTGAAACCATCGTCATCGGTGGCGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTATTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.611
  comQ Acinetobacter baylyi ADP1

42.952

100

0.462
  pilQ Acinetobacter baumannii D1279779

41.048

100

0.445
  pilQ Acinetobacter baumannii strain A118

41.048

100

0.445
  pilQ Legionella pneumophila strain Lp02

43.301

99.052

0.429
  pilQ Legionella pneumophila strain ERS1305867

43.301

99.052

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.928

98.341

0.412
  pilQ Vibrio cholerae strain A1552

41.928

98.341

0.412
  pilQ Vibrio campbellii strain DS40M4

40.662

100

0.408

Multiple sequence alignment