Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXA56_RS00265 Genome accession   NZ_CP031609
Coordinates   54299..55684 (+) Length   461 a.a.
NCBI ID   WP_096983547.1    Uniprot ID   -
Organism   Escherichia coli strain N3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 49299..60684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXA56_RS00235 (AXA56_00235) aroP 49847..51220 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  AXA56_RS00240 (AXA56_00240) ampE 51263..52117 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  AXA56_RS00245 (AXA56_00245) ampD 52114..52665 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AXA56_RS00250 (AXA56_00250) nadC 52753..53646 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AXA56_RS00260 (AXA56_00260) pilA 53849..54289 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  AXA56_RS00265 (AXA56_00265) pilB 54299..55684 (+) 1386 WP_096983547.1 type II secretion system protein GspE Machinery gene
  AXA56_RS00270 (AXA56_00270) hofC 55674..56876 (+) 1203 WP_116836068.1 protein transport protein HofC -
  AXA56_RS00275 (AXA56_00275) guaC 56911..57954 (-) 1044 WP_001217337.1 GMP reductase -
  AXA56_RS25150 - 58110..58154 (-) 45 WP_120795372.1 protein YacM -
  AXA56_RS00280 (AXA56_00280) coaE 58179..58799 (+) 621 WP_001269517.1 dephospho-CoA kinase -
  AXA56_RS00285 (AXA56_00285) zapD 58799..59542 (+) 744 WP_001194731.1 cell division protein ZapD -
  AXA56_RS00290 (AXA56_00290) yacG 59552..59749 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  AXA56_RS00295 (AXA56_00295) mutT 59837..60235 (-) 399 WP_000736011.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50694.43 Da        Isoelectric Point: 6.7135

>NTDB_id=266418 AXA56_RS00265 WP_096983547.1 54299..55684(+) (pilB) [Escherichia coli strain N3]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPANNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHTLQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=266418 AXA56_RS00265 WP_096983547.1 54299..55684(+) (pilB) [Escherichia coli strain N3]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAGATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGAACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATACATTGCAACAAC
CACAGGGATTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAACTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCAATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

41.278

88.286

0.364
  pilB Acinetobacter baumannii D1279779

43.864

83.08

0.364