Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MC60_RS15715 Genome accession   NZ_CP026055
Coordinates   3445103..3446809 (-) Length   568 a.a.
NCBI ID   WP_042864859.1    Uniprot ID   -
Organism   Aeromonas caviae strain FDAARGOS_72     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3440103..3451809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MC60_RS15685 (MC60_015685) mutT 3440721..3441125 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  MC60_RS15690 (MC60_015690) yacG 3441183..3441377 (-) 195 WP_010675826.1 DNA gyrase inhibitor YacG -
  MC60_RS15695 (MC60_015695) zapD 3441388..3442110 (-) 723 WP_010675825.1 cell division protein ZapD -
  MC60_RS15700 (MC60_015700) coaE 3442148..3442762 (-) 615 WP_042864857.1 dephospho-CoA kinase -
  MC60_RS15705 (MC60_015705) pilD 3442781..3443656 (-) 876 WP_042864858.1 prepilin peptidase Machinery gene
  MC60_RS15710 (MC60_015710) pilC 3443734..3444975 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  MC60_RS15715 (MC60_015715) pilB 3445103..3446809 (-) 1707 WP_042864859.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MC60_RS15720 (MC60_015720) tapA 3446813..3447307 (-) 495 WP_041213996.1 type IVa pilus major pilin TapA -
  MC60_RS15725 (MC60_015725) nadC 3447618..3448481 (-) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MC60_RS15730 (MC60_015730) - 3448485..3448955 (-) 471 WP_042864862.1 TIGR02281 family clan AA aspartic protease -
  MC60_RS15735 (MC60_015735) ampD 3449084..3449656 (+) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MC60_RS15745 (MC60_015745) pdhR 3449933..3450697 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=266180 MC60_RS15715 WP_042864859.1 3445103..3446809(-) (pilB) [Aeromonas caviae strain FDAARGOS_72]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGIEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=266180 MC60_RS15715 WP_042864859.1 3445103..3446809(-) (pilB) [Aeromonas caviae strain FDAARGOS_72]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCTGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCTTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTACATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAGCGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATTTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGCTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGTTTGCGGCTGTTCAAACCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAAATTGCCG
CCATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCATCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

55.044

99.472

0.548
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.707

99.648

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.266

99.12

0.498
  pilF Neisseria gonorrhoeae MS11

46.737

99.824

0.467
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.794

97.359

0.368

Multiple sequence alignment