Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C2H95_RS05090 Genome accession   NZ_CP026037
Coordinates   979766..980215 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain PK5_17     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 974766..985215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H95_RS05050 - 975087..975284 (+) 198 WP_139026565.1 hypothetical protein -
  C2H95_RS05055 (C2H95_05055) yckC 975304..975759 (+) 456 WP_049140212.1 RDD family protein -
  C2H95_RS05060 (C2H95_05060) yckD 975840..976172 (+) 333 WP_032724908.1 YckD family protein -
  C2H95_RS05065 (C2H95_05065) - 976300..976722 (+) 423 WP_170939093.1 MarR family transcriptional regulator -
  C2H95_RS05070 (C2H95_05070) - 976710..977051 (+) 342 WP_032724911.1 DUF3147 family protein -
  C2H95_RS05075 (C2H95_05075) - 977070..977798 (+) 729 WP_021481826.1 AAA family ATPase -
  C2H95_RS05080 (C2H95_05080) bglC 977871..979304 (+) 1434 WP_072175231.1 6-phospho-beta-glucosidase -
  C2H95_RS05085 (C2H95_05085) nin/comJ 979341..979739 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  C2H95_RS05090 (C2H95_05090) nucA/comI 979766..980215 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  C2H95_RS05095 (C2H95_05095) tlpC 980383..982104 (-) 1722 WP_021481821.1 methyl-accepting chemotaxis protein TlpC -
  C2H95_RS05100 (C2H95_05100) hxlB 982214..982771 (-) 558 WP_021481820.1 6-phospho-3-hexuloisomerase -
  C2H95_RS05105 (C2H95_05105) hxlA 982777..983409 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  C2H95_RS05110 (C2H95_05110) hxlR 983641..984003 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=265679 C2H95_RS05090 WP_014475770.1 979766..980215(-) (nucA/comI) [Bacillus subtilis strain PK5_17]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=265679 C2H95_RS05090 WP_014475770.1 979766..980215(-) (nucA/comI) [Bacillus subtilis strain PK5_17]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGATGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987

Multiple sequence alignment