Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1S76_RS14245 Genome accession   NZ_CP025796
Coordinates   2960554..2962239 (-) Length   561 a.a.
NCBI ID   WP_045413053.1    Uniprot ID   -
Organism   Vibrio owensii strain 051011B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2955554..2967239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S76_RS14215 rplS 2955899..2956252 (+) 354 WP_005438036.1 50S ribosomal protein L19 -
  C1S76_RS14220 yacG 2956715..2956909 (-) 195 WP_009707573.1 DNA gyrase inhibitor YacG -
  C1S76_RS14225 zapD 2956972..2957712 (-) 741 WP_009707572.1 cell division protein ZapD -
  C1S76_RS14230 coaE 2957740..2958354 (-) 615 WP_045413058.1 dephospho-CoA kinase -
  C1S76_RS14235 pilD 2958355..2959224 (-) 870 WP_045413056.1 prepilin peptidase Machinery gene
  C1S76_RS14240 pilC 2959297..2960520 (-) 1224 WP_009707569.1 type II secretion system F family protein Machinery gene
  C1S76_RS14245 pilB 2960554..2962239 (-) 1686 WP_045413053.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1S76_RS14250 - 2962243..2962683 (-) 441 WP_045413051.1 type IV pilin protein -
  C1S76_RS14260 nadC 2962948..2963835 (-) 888 WP_039840279.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1S76_RS14265 ampD 2963928..2964479 (+) 552 WP_045413040.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1S76_RS14270 pdhR 2964884..2965651 (+) 768 WP_045413037.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62252.23 Da        Isoelectric Point: 5.6668

>NTDB_id=263901 C1S76_RS14245 WP_045413053.1 2960554..2962239(-) (pilB) [Vibrio owensii strain 051011B]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSRLKEINQDELAS
LVEIGDDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRQPQEPNAQLQHIGIINTEH
VFKANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIAKGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=263901 C1S76_RS14245 WP_045413053.1 2960554..2962239(-) (pilB) [Vibrio owensii strain 051011B]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAACTGTTACTTGAGCAAGC
CAAAGCTTCTGGTATATCAATGCCAGAAGCTTTACTCAATTCTGGCTTTTTTCAATCTCATGAATTAACGGAGCACCTGA
GCTCTGTTTTTGGGCTTTCTTGCACTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACGCTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACCTCATCCACTTTGATTCTCGCGGTTGCTGACCCTACCAACCT
TCAAGCCGAAGACGACTTTCGTTTTGCTACCGGCTTACAAGTAGAGCTAGTTCTGGCGGATTTTCGAGAGCTGACAGCTG
CCATTCGGCGCTTATATGGCCGAGCACTTGGGCAGGAAAAGTCAAGGTTAAAAGAAATAAACCAAGATGAGCTCGCAAGC
TTGGTTGAGATTGGAGATGATGAGATTGAGAACATTGAAGATCTCAGTCAAGACGAATCGCCCGTCAGTCGCTATATCAA
CCAGATCCTGCTCGATGCCGTTCGTAAAGGGGCTTCCGATATTCACTTTGAACCCTACGAAAACATGTACCGAGTCCGTT
TACGCTGCGATGGCATCTTGATTGAAATTCAACAGCCTCCGAGCCATTTAAGCCGTCGCCTTTCCGCACGAATAAAAATC
CTATCTAAGCTCGATATTGCTGAACGTCGCTTACCTCAGGATGGACGAATCAAACTTAAGCTAAACCAAGACACTGCGAT
AGATATGCGTGTTTCTACACTGCCAACTCTGTTTGGAGAAAAGATCGTCCTGCGTTTGCTCGACAGTAGTTCTGCAGCAT
TAGATATCAATAAACTTGGTTATAGCGACCAACAGAAGCAGCTTTATTTGGATGCTCTTCGTCGGCCACAAGGCATGATT
TTGATGACGGGTCCAACAGGCAGTGGCAAAACCGTTTCTCTTTACACAGGGCTAAGCATTCTTAACAAACCAGAGATCAA
CATCTCAACTGCGGAAGACCCAGTGGAGATAAACTTACCCGGCATTAACCAAGTTCAAGTTCAGCCGAAGATCGGTTTTG
GTTTTGCTGAAGCACTTCGTTCTTTCTTACGTCAGGATCCTGATGTGGTCATGGTGGGTGAGATCCGAGATCTCGATACG
GCAGAAATCGCGATCAAAGCATCACAAACTGGTCACTTGGTTCTTTCCACGTTACATACGAACTCTGCCGCTGAAACCGT
AATCCGCTTATCCAACATGGGTGTAGAAAGCTTTAACCTTGCCTCCTCACTCAGTTTGATCATCGCCCAACGGTTAGCAC
GCAAATTGTGCCCGCACTGTAGACAACCGCAAGAGCCAAATGCCCAACTGCAGCATATTGGGATCATCAACACAGAGCAT
GTCTTTAAAGCCAATCCAGATGGTTGTAACGAGTGTACCCACGGTTATTCAGGTCGAACGGGCATCTATGAAGTGATGAA
GTTTGATGAGTCCCTATCTGAGGCGCTCATAAAAGGAGCTTCAGTACACGAATTAGAAAAACTCGCCATCGCCAAAGGGA
TGCAAACCCTACAAATGTCTGGCATAGAAAAACTCAAACAAGGCGTCACTAGCTTCAGCGAATTACAACGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.613

100

0.966
  pilB Vibrio parahaemolyticus RIMD 2210633

89.305

100

0.893
  pilB Vibrio cholerae strain A1552

73.665

100

0.738
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Legionella pneumophila strain ERS1305867

48.524

96.613

0.469
  pilB Acinetobacter baylyi ADP1

48.339

96.613

0.467
  pilF Neisseria gonorrhoeae MS11

42.908

100

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.702

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.136

99.643

0.36

Multiple sequence alignment