Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1S74_RS13965 Genome accession   NZ_CP025794
Coordinates   2807845..2809530 (-) Length   561 a.a.
NCBI ID   WP_045403773.1    Uniprot ID   -
Organism   Vibrio hyugaensis strain 090810a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2802845..2814530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S74_RS13935 rplS 2803318..2803671 (+) 354 WP_038870475.1 50S ribosomal protein L19 -
  C1S74_RS13940 yacG 2804006..2804200 (-) 195 WP_038870477.1 DNA gyrase inhibitor YacG -
  C1S74_RS13945 zapD 2804266..2805006 (-) 741 WP_045404061.1 cell division protein ZapD -
  C1S74_RS13950 coaE 2805034..2805648 (-) 615 WP_042603882.1 dephospho-CoA kinase -
  C1S74_RS13955 pilD 2805649..2806518 (-) 870 WP_045403769.1 A24 family peptidase Machinery gene
  C1S74_RS13960 pilC 2806590..2807813 (-) 1224 WP_045403771.1 type II secretion system F family protein Machinery gene
  C1S74_RS13965 pilB 2807845..2809530 (-) 1686 WP_045403773.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1S74_RS13970 - 2809537..2809980 (-) 444 WP_045403775.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C1S74_RS13975 nadC 2810245..2811132 (-) 888 WP_045403776.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1S74_RS13980 ampD 2811225..2811776 (+) 552 WP_045403778.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1S74_RS13985 pdhR 2812181..2812948 (+) 768 WP_005438026.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61999.70 Da        Isoelectric Point: 5.3949

>NTDB_id=263854 C1S74_RS13965 WP_045403773.1 2807845..2809530(-) (pilB) [Vibrio hyugaensis strain 090810a]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPINRSSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELSAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGADEIDNIEDLSQDEAPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILVESQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLNIDRLGYSDEQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLSLLNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFSEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPQCKQPQEPNAQLQHIGIHSTEN
IFQANPDGCNECTQGYSGRTGIYEVMRFDETLSEALIKGASVHELEKLAIKNGMHTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=263854 C1S74_RS13965 WP_045403773.1 2807845..2809530(-) (pilB) [Vibrio hyugaensis strain 090810a]
ATGCTAACAAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACCTTCTCACAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTGACGGAACACTTAA
GCTCTGTCTTTGGGCTTTCTTGCACCTCTCTCAATCAGTACGAATACGCTTCGCTCTGCCAAACGCTGGGCTTACGTGAT
CTAATCACTCGCCATAATGCTCTGCCGATCAATCGCAGTTCTTCGACTTTGATCTTGGCGGTGGCTGATCCTACTAATCT
CCAAGCTGAAGACGACTTTCGTTTTGCGACAGGACTACAGGTTGAGCTTGTTCTTGCGGATTTTCGTGAACTCAGTGCGG
CAATTAGGCGCTTATATGGTCGAGCACTAGGACAGGAAAAGTCCGGATTAAAAGAGATCAACCAAGATGAACTAGCAGGT
CTGGTTGATATTGGTGCAGATGAAATCGACAACATCGAAGATCTCAGCCAAGACGAAGCACCTGTCAGTCGTTACATTAA
TCAGATTTTGTTGGATGCCGTGCGTAAAGGCGCTTCGGATATTCACTTTGAACCCTACGAAAACATGTATCGAGTGCGCT
TACGTTGTGATGGAATTCTCGTTGAAAGCCAACAACCACCAAGTCATTTAAGTCGCCGTTTATCGGCGCGTATTAAAATC
TTATCCAAACTCGATATCGCCGAACGACGCTTACCGCAAGATGGTCGTATCAAGCTTAAGCTAAACCAAGATACTGCGAT
CGATATGCGCGTATCGACACTACCAACGCTATTTGGCGAAAAAATAGTACTGCGTTTGCTCGACAGCAGCTCCGCCTCAC
TAAATATTGACCGCTTGGGTTACAGCGATGAGCAGAAGCAACTTTATCTAAATGCGTTAAAACGGCCACAAGGCATGATT
TTAATGACAGGCCCGACGGGAAGTGGCAAAACCGTCTCGCTCTATACAGGCTTAAGTCTTCTAAACAAAACAGAGATCAA
TATCTCTACTGCAGAAGACCCCGTTGAAATTAACCTGCCTGGCATCAACCAAGTGCAAGTCCAGCCCAAAATTGGCTTTG
GTTTTTCTGAAGCACTTCGTTCTTTCTTACGTCAGGATCCTGATGTGGTCATGGTGGGTGAGATCCGCGATCTAGACACG
GCAGAAATCGCCATTAAAGCCTCACAAACTGGCCACTTAGTTCTGTCTACTCTGCACACCAACTCTGCCGCTGAAACCGT
GATTCGCTTATCCAATATGGGGGTTGAGAGCTTTAACCTTGCTTCATCATTGAGCTTGATCATCGCTCAACGCTTGGCAA
GAAAACTATGCCCTCAGTGCAAACAACCTCAAGAGCCGAATGCACAACTGCAACATATTGGCATCCACTCCACGGAAAAC
ATCTTTCAAGCGAACCCAGATGGCTGTAACGAATGTACCCAAGGTTATTCTGGTCGAACCGGCATTTATGAAGTGATGCG
CTTTGACGAGACGCTTTCCGAAGCACTAATCAAAGGCGCATCCGTTCATGAGTTAGAAAAACTTGCTATCAAAAACGGCA
TGCACACCTTACAAATGTCCGGTATAGAAAAACTTAAACAAGGCATTACCAGCTTTAGCGAATTACAACGCGTTCTCTAC
TTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

94.118

100

0.941
  pilB Vibrio parahaemolyticus RIMD 2210633

89.305

100

0.893
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Legionella pneumophila strain ERS1305867

49.538

96.435

0.478
  pilB Acinetobacter baylyi ADP1

47.842

99.109

0.474
  pilB Acinetobacter baumannii D1279779

50.864

92.87

0.472
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

37.542

100

0.398
  pilF Thermus thermophilus HB27

36.429

99.822

0.364

Multiple sequence alignment