Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CSAL_RS11075 Genome accession   NC_007963
Coordinates   2447058..2448797 (+) Length   579 a.a.
NCBI ID   WP_011507471.1    Uniprot ID   -
Organism   Chromohalobacter israelensis DSM 3043     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442058..2453797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSAL_RS11055 (Csal_2170) - 2442923..2444101 (+) 1179 WP_011507467.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  CSAL_RS11060 (Csal_2171) - 2444106..2445587 (-) 1482 WP_011507468.1 TrkH family potassium uptake protein -
  CSAL_RS11065 (Csal_2172) - 2445855..2446142 (+) 288 WP_011507469.1 hypothetical protein -
  CSAL_RS11070 (Csal_2173) - 2446272..2446712 (-) 441 WP_011507470.1 pilin -
  CSAL_RS11075 (Csal_2174) pilB 2447058..2448797 (+) 1740 WP_011507471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSAL_RS11080 (Csal_2175) pilC 2448830..2450062 (+) 1233 WP_011507472.1 type II secretion system F family protein Machinery gene
  CSAL_RS11085 (Csal_2176) pilD 2450178..2451020 (+) 843 WP_011507473.1 A24 family peptidase Machinery gene
  CSAL_RS11090 (Csal_2177) coaE 2451031..2451669 (+) 639 WP_011507474.1 dephospho-CoA kinase -
  CSAL_RS11095 (Csal_2178) yacG 2451671..2451901 (+) 231 WP_011507475.1 DNA gyrase inhibitor YacG -
  CSAL_RS11100 (Csal_2179) - 2452017..2452961 (-) 945 WP_011507476.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62934.86 Da        Isoelectric Point: 5.0125

>NTDB_id=25740 CSAL_RS11075 WP_011507471.1 2447058..2448797(+) (pilB) [Chromohalobacter israelensis DSM 3043]
MPLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPARAATQVAAWEYGLPFVDLEA
VDLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPATLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETH
GMLEALGDASEALETLDLDDVEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVAR
PPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLRLLDAGATRLGIDALGFSEAQ
RAMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDARNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQ
DPDVVMVGEVRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVE
LPRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNSLELGELARQEGHPDLRRSGL
SKVLAGITSLEEINRVVLE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=25740 CSAL_RS11075 WP_011507471.1 2447058..2448797(+) (pilB) [Chromohalobacter israelensis DSM 3043]
ATGCCATTGTCTTCATCATCCACCTCCGATACGGAGGCATCACCGCTGAAAGGGCTCGCCGCGCGCCTGGTGCAGGAAGG
TCTGCTCGAGGAACAGCAGGCGCGCAGCGCCCTGGAGGCCGTACGCGAAAGCCACCAGTCGATGCTCGAGTATGTCATCG
AGCGCCAGTGGGTCCCTGCCCGCGCGGCGACCCAGGTTGCGGCCTGGGAGTACGGTCTGCCGTTCGTCGATCTCGAGGCC
GTCGACCTCGGCCGGCTCCCCACCGGTGAGGGCCTGCCCGATGCGATGATACGTCGTCTGGGCATACTGCCCCTGGCGCG
CAGCGAGCGGCATATCAGCGTGGCGGTTCCCTACCCCGCGACCCTGGCGAAACTGGACGAACTGCAGTTCAGTACCGGCC
TTTCCGCCGAGGGGGTCCTGGTCGCCGCCGATCAGTTGCAACCCGCCATCGACAGCCACCTTGCCCAGCGCGAGACGCAC
GGCATGCTCGAGGCCCTTGGCGATGCCAGCGAAGCGCTCGAGACGCTGGATCTCGACGATGTCGAGCCTCTCGAGGATGG
GCTCGGTACCGAGGACGACGATGCGCCGGTGGTGCGCTTCGTGCACAAGATGATGATCGATGCGGCCCGTCGCGGCGCCT
CGGACATTCACTTCGAGCCGTACGAGTCGACCTTTCGCATTCGTTTTCGCATCGATGGCATCCTGGTCGAGGTCGCACGC
CCACCATTCAACCTGCGTGCGCGTCTGTCGGCACGCCTCAAGGTCATGTCGCGCCTGGACATCTCCGAACGCCGCCTGCC
TCAGGACGGCGCGATCAAGCTGCGGCTGTCGGCATCGCGAACCATCGATTTCCGCGTCAGCACCTTGCCCACGGTCCATG
GCGAGAAGATCGTGCTGCGTCTGCTGGATGCCGGCGCCACGCGCCTGGGGATCGATGCACTGGGGTTCAGCGAGGCGCAG
CGCGCGATGTTCGAGCACGCCCTGGCACAGCCCCAGGGCATGATTCTCGCCACCGGCCCCACCGGCAGCGGCAAGACCGT
GACCTTGTACACTGGGCTCAATATCCTCAACACCGACGCGCGCAACATTTCCACCGCCGAGGATCCCGTCGAACTCAAGC
TCGACGGCATCAACCAGGTCAGCGTTCAGCCGAAGATCGGGCTGGATTTCGCCAATGCCTTGCGCGCCTTCCTGCGCCAG
GACCCCGACGTGGTCATGGTCGGCGAGGTGCGCGACCTGGAGACCGCCGAAATCGCCGTCAAGGCCGCGCAAACCGGACA
CCTGGTGCTCTCGACGCTGCATACGAATTCGGCGGCGGAAACGCTGACGCGCCTGCGCAACATGGGGGTGGCGGCCTACA
ACATCGCCAGTTCGGTGAGCCTGATCGTCGCCCAGCGGCTCGTGCGACAACTCTGCCCGCACTGCAAGACGCCCGTCGAG
CTGCCCCGGGAGCTCTTCATCGAGGCCGGCTTCACGTCCACCGACATCGAATCGACGACCTGCTATCGTGCCGCGGGCTG
CGCCCAGTGTACCCACGGGTACAAGGGACGCGTGGGAATCTACGAGGTACTGCCCGTCAGCGAGGCCATGAGCAAGTTGA
TCATGGCCGACGGCAATTCTCTGGAACTGGGGGAACTGGCCCGTCAAGAAGGCCACCCCGATTTAAGACGCAGCGGACTG
AGCAAGGTGCTCGCCGGCATCACCAGCCTCGAGGAAATCAACCGCGTGGTGCTGGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.098

98.791

0.515
  pilB Acinetobacter baylyi ADP1

50.521

99.482

0.503
  pilB Vibrio cholerae strain A1552

51.413

97.755

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50.088

97.582

0.489
  pilB Vibrio campbellii strain DS40M4

50.088

97.582

0.489
  pilB Acinetobacter baumannii D1279779

49.912

97.582

0.487
  pilF Neisseria gonorrhoeae MS11

46.25

96.718

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

37.377

100

0.394
  pilF Thermus thermophilus HB27

37.655

97.237

0.366

Multiple sequence alignment