Detailed information NTG0257077

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CWD84_RS09000
• Genome accession: NZ_CP025001
• Coordinates: 1656062..1656676 (+)
• Length: 204 a.a.
• NCBI ID: WP_060964714.1
• Uniprot ID: A0AAI8HMV7
• Organism: Bacillus siamensis strain SCSIO 05746
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1651062..1661676

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWD84_RS08965 (CWD84_08965)	aroE	1651750..1652592 (+)	843	WP_060964710.1	shikimate dehydrogenase	-
CWD84_RS08970 (CWD84_08970)	yhbY	1652586..1652876 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CWD84_RS08975 (CWD84_08975)	-	1652887..1653456 (+)	570	WP_016938889.1	nicotinate-nucleotide adenylyltransferase	-
CWD84_RS08980 (CWD84_08980)	yqeK	1653446..1654006 (+)	561	WP_060964711.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CWD84_RS08985 (CWD84_08985)	rsfS	1654023..1654379 (+)	357	WP_016938891.1	ribosome silencing factor	-
CWD84_RS08990 (CWD84_08990)	-	1654376..1655113 (+)	738	WP_060964712.1	class I SAM-dependent DNA methyltransferase	-
CWD84_RS08995 (CWD84_08995)	comER	1655182..1656003 (-)	822	WP_060964713.1	late competence protein ComER	-
CWD84_RS09000 (CWD84_09000)	comEA	1656062..1656676 (+)	615	WP_060964714.1	helix-hairpin-helix domain-containing protein	Machinery gene
CWD84_RS09005 (CWD84_09005)	-	1656742..1657311 (+)	570	WP_016938895.1	ComE operon protein 2	-
CWD84_RS09010 (CWD84_09010)	comEC	1657312..1659663 (+)	2352	WP_060964715.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CWD84_RS09015 (CWD84_09015)	-	1659682..1659816 (-)	135	WP_003152870.1	YqzM family protein	-
CWD84_RS22095	-	1659857..1660011 (+)	155	Protein_1777	hypothetical protein	-
CWD84_RS09020 (CWD84_09020)	holA	1660051..1661092 (+)	1042	Protein_1778	DNA polymerase III subunit delta	-
CWD84_RS09025 (CWD84_09025)	rpsT	1661110..1661376 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22272.36 Da
• Isoelectric Point: 4.7944

>NTDB_id=257077 CWD84_RS09000 WP_060964714.1 1656062..1656676(+) (comEA) [Bacillus siamensis strain SCSIO 05746]
MTKAMMTEQMRRNLKILGVCLAAGAVFICIWLFRPEKEEPVKQQSVLGQTASASGTDKTDTADDKIMIDMKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEEENRPKTAAGEKEDAPVNINTATLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=257077 CWD84_RS09000 WP_060964714.1 1656062..1656676(+) (comEA) [Bacillus siamensis strain SCSIO 05746]
ATGACGAAAGCGATGATGACGGAGCAGATGCGGCGGAACCTGAAAATTCTGGGTGTTTGTCTTGCGGCAGGAGCGGTTTT
TATCTGCATTTGGCTGTTTCGTCCTGAAAAAGAAGAACCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGGCACGGACAAAACAGACACCGCAGACGATAAGATTATGATTGATATGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACACAAGCCATTGAAAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAAGACGGGACGGTCGTTTATATTCCGAGCGAAGGCGAAGAGGAGAATCGCCCGAAAACCG
CAGCAGGTGAGAAGGAGGATGCGCCTGTCAATATCAATACGGCAACTCTGGACGAACTTCAGGCGATTTCCGGTGTGGGG
CAGAAAAAAGCGGAAGCAATTATCGCCTACCGGGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (142-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.5	98.039	0.574
comEA	Lactococcus lactis subsp. cremoris KW2	36.715	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.232	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.749	100	0.363