Detailed information NTG0256818

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CV098_RS08650
• Genome accession: NZ_CP024958
• Coordinates: 1823884..1824531 (-)
• Length: 215 a.a.
• NCBI ID: WP_017864913.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain G423
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1818884..1829531

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV098_RS08625 (CV098_08625)	atpF	1818953..1819459 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
CV098_RS08630 (CV098_08630)	atpB	1819474..1820187 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
CV098_RS08635 (CV098_08635)	-	1820232..1820447 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
CV098_RS08640 (CV098_08640)	-	1820632..1821408 (-)	777	WP_017864911.1	alpha/beta hydrolase	-
CV098_RS08645 (CV098_08645)	comEC	1821747..1823903 (-)	2157	WP_017864912.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CV098_RS08650 (CV098_08650)	comEA	1823884..1824531 (-)	648	WP_017864913.1	ComEA family DNA-binding protein	Machinery gene
CV098_RS08655 (CV098_08655)	-	1824591..1825952 (-)	1362	WP_017864914.1	ABC transporter permease	-
CV098_RS08660 (CV098_08660)	-	1825949..1826881 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
CV098_RS08665 (CV098_08665)	-	1826987..1827385 (-)	399	WP_004255236.1	hypothetical protein	-
CV098_RS08670 (CV098_08670)	-	1827489..1828052 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
CV098_RS08675 (CV098_08675)	-	1828225..1829403 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23598.73 Da
• Isoelectric Point: 5.0013

>NTDB_id=256818 CV098_RS08650 WP_017864913.1 1823884..1824531(-) (comEA) [Lactococcus lactis subsp. lactis strain G423]
MDKILEKVKEYWKMIVLVVCGLIAGGIFHVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=256818 CV098_RS08650 WP_017864913.1 1823884..1824531(-) (comEA) [Lactococcus lactis subsp. lactis strain G423]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTCACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	38.696	100	0.414
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367