Detailed information NTG0256721

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CV702_RS08915
• Genome accession: NZ_CP024954
• Coordinates: 1880141..1880788 (-)
• Length: 215 a.a.
• NCBI ID: WP_017864913.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain F44
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1875141..1885788

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV702_RS08890 (CV702_08900)	atpF	1875210..1875716 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
CV702_RS08895 (CV702_08905)	atpB	1875731..1876444 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
CV702_RS08900 (CV702_08910)	-	1876489..1876704 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
CV702_RS08905 (CV702_08915)	-	1876889..1877665 (-)	777	WP_017864911.1	alpha/beta hydrolase	-
CV702_RS08910 (CV702_08920)	comEC	1878004..1880160 (-)	2157	WP_017864912.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CV702_RS08915 (CV702_08925)	comEA	1880141..1880788 (-)	648	WP_017864913.1	ComEA family DNA-binding protein	Machinery gene
CV702_RS08920 (CV702_08930)	-	1880848..1882209 (-)	1362	WP_017864914.1	ABC transporter permease	-
CV702_RS08925 (CV702_08935)	-	1882206..1883138 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
CV702_RS08930 (CV702_08940)	-	1883244..1883642 (-)	399	WP_004255236.1	hypothetical protein	-
CV702_RS08935 (CV702_08945)	-	1883746..1884309 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
CV702_RS08940 (CV702_08950)	-	1884482..1885660 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23598.73 Da
• Isoelectric Point: 5.0013

>NTDB_id=256721 CV702_RS08915 WP_017864913.1 1880141..1880788(-) (comEA) [Lactococcus lactis subsp. lactis strain F44]
MDKILEKVKEYWKMIVLVVCGLIAGGIFHVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=256721 CV702_RS08915 WP_017864913.1 1880141..1880788(-) (comEA) [Lactococcus lactis subsp. lactis strain F44]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTCACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	38.696	100	0.414
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367