Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CVS42_RS18725 Genome accession   NZ_CP024933
Coordinates   4055374..4057080 (+) Length   568 a.a.
NCBI ID   WP_005356616.1    Uniprot ID   -
Organism   Aeromonas veronii strain X12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4050374..4062080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CVS42_RS18695 (CVS42_18705) pdhR 4051583..4052347 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  CVS42_RS18700 (CVS42_18710) ampD 4052626..4053201 (-) 576 WP_100646863.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CVS42_RS18705 (CVS42_18715) - 4053332..4053802 (+) 471 WP_005336129.1 TIGR02281 family clan AA aspartic protease -
  CVS42_RS18710 (CVS42_18720) nadC 4053806..4054669 (+) 864 WP_040068931.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CVS42_RS22720 (CVS42_18730) - 4054985..4055371 (+) 387 WP_100646864.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CVS42_RS18725 (CVS42_18735) pilB 4055374..4057080 (+) 1707 WP_005356616.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CVS42_RS18730 (CVS42_18740) pilC 4057321..4058562 (+) 1242 WP_100646865.1 type II secretion system F family protein Machinery gene
  CVS42_RS18735 (CVS42_18745) pilD 4058624..4059496 (+) 873 WP_100646866.1 A24 family peptidase Machinery gene
  CVS42_RS18740 (CVS42_18750) coaE 4059510..4060124 (+) 615 WP_100646867.1 dephospho-CoA kinase -
  CVS42_RS18745 (CVS42_18755) zapD 4060162..4060884 (+) 723 WP_021231281.1 cell division protein ZapD -
  CVS42_RS18750 (CVS42_18760) yacG 4060894..4061088 (+) 195 WP_100646869.1 DNA gyrase inhibitor YacG -
  CVS42_RS18755 (CVS42_18765) mutT 4061166..4061570 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62563.90 Da        Isoelectric Point: 5.3192

>NTDB_id=256457 CVS42_RS18725 WP_005356616.1 4055374..4057080(+) (pilB) [Aeromonas veronii strain X12]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=256457 CVS42_RS18725 WP_005356616.1 4055374..4057080(+) (pilB) [Aeromonas veronii strain X12]
ATGACCTCTAGTCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAAAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAAGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTCGGCTTCAGCTTTGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGTGTCAATACCACCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATTGCCACACCGCCGGTTAACCTTTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGGCTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCACTCTCGAAACCACAG
GGGATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTAGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCAGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTATGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAAGAGGGGATGCGTACCTTACGTACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTTAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment