Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RFER_RS14685 Genome accession   NC_007908
Coordinates   3213157..3214374 (+) Length   405 a.a.
NCBI ID   WP_011465180.1    Uniprot ID   Q21UD9
Organism   Rhodoferax ferrireducens T118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3208157..3219374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFER_RS14670 (Rfer_2900) - 3209218..3209493 (+) 276 WP_041790807.1 type B 50S ribosomal protein L31 -
  RFER_RS14675 (Rfer_2901) - 3209561..3211279 (+) 1719 WP_041790809.1 hypothetical protein -
  RFER_RS14680 (Rfer_2902) pilB 3211403..3213136 (+) 1734 WP_011465179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RFER_RS14685 (Rfer_2903) pilC 3213157..3214374 (+) 1218 WP_011465180.1 type II secretion system F family protein Machinery gene
  RFER_RS14690 (Rfer_2904) - 3214374..3215216 (+) 843 WP_011465181.1 prepilin peptidase -
  RFER_RS14695 (Rfer_2905) coaE 3215220..3215858 (+) 639 WP_011465182.1 dephospho-CoA kinase -
  RFER_RS14700 (Rfer_2906) zapD 3215864..3216622 (+) 759 WP_011465183.1 cell division protein ZapD -
  RFER_RS14705 (Rfer_2907) - 3216619..3216837 (+) 219 WP_011465184.1 DNA gyrase inhibitor YacG -
  RFER_RS14710 (Rfer_2908) - 3216877..3217344 (-) 468 WP_244095731.1 NUDIX domain-containing protein -
  RFER_RS14715 (Rfer_2909) - 3217353..3218231 (-) 879 WP_011465186.1 ATP-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44740.88 Da        Isoelectric Point: 9.6469

>NTDB_id=25554 RFER_RS14685 WP_011465180.1 3213157..3214374(+) (pilC) [Rhodoferax ferrireducens T118]
MATEKSKKIKEFVFEWEGKDRNGKQVRGETRAVGEHQVQSMLRRQGVMSTKIKKRRMRAGKSIKPKDLAIFTRQLATMMK
AGVPLLQAFDIVGRGNPNPSVTKLLNDIRTDVETGTSLSVAFRKFPLYFDNLYCNLIEAGESAGILDQLLDRLAVYMEKT
EAIKSKIKSALMYPISVLIVAFVVTAVIMIFVVPAFKEVFSNFGADLPAPTLVVIAISEIFVKYWWLIFGAIGGGFYFFM
QAWRRNEKMQHVMDRIMLKMPIFGVLVDKSCIARWTRTLSTMFAAGVPLVEALDSVGGAAGNSVYEIATKKIQQEVSTGT
ALTVAMTNTNLFPSMVLQMCSIGEESGSIDHMLGKAADFYEAEVDDMVAGISSLMEPIIIVILGVVIGGIVVAMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=25554 RFER_RS14685 WP_011465180.1 3213157..3214374(+) (pilC) [Rhodoferax ferrireducens T118]
ATGGCAACTGAAAAGTCAAAAAAAATCAAGGAATTTGTCTTTGAGTGGGAAGGCAAGGACCGCAATGGCAAGCAGGTACG
CGGGGAAACCCGAGCGGTTGGCGAGCACCAGGTTCAGTCCATGCTGCGTCGCCAGGGCGTCATGTCAACCAAGATCAAGA
AACGCCGGATGCGCGCCGGGAAATCCATCAAGCCCAAAGACCTCGCCATCTTCACGCGCCAACTCGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAAGCGTTTGACATCGTCGGGCGCGGCAACCCCAATCCCAGCGTCACCAAACTGCTCAACGA
TATTCGTACCGATGTTGAAACCGGAACTTCATTGAGTGTTGCCTTTCGCAAGTTCCCGCTGTATTTCGACAACCTCTATT
GCAATCTGATCGAGGCCGGGGAGTCCGCCGGTATTCTCGACCAGCTGCTCGACCGGCTGGCGGTTTACATGGAGAAGACC
GAAGCCATCAAGTCCAAAATCAAGTCGGCCTTGATGTATCCGATTTCGGTCCTGATCGTCGCTTTTGTGGTCACGGCTGT
GATCATGATTTTTGTCGTGCCGGCCTTCAAGGAGGTGTTCTCGAATTTTGGCGCCGACCTGCCGGCGCCCACGCTGGTCG
TGATTGCCATCAGCGAAATTTTCGTCAAGTATTGGTGGCTTATTTTTGGTGCCATTGGCGGCGGCTTTTACTTTTTCATG
CAAGCCTGGCGGCGCAACGAAAAGATGCAACACGTGATGGACCGCATCATGCTCAAGATGCCCATTTTTGGCGTGCTGGT
CGACAAATCATGCATTGCCCGCTGGACACGCACCCTGTCGACCATGTTCGCCGCCGGTGTACCGCTGGTCGAAGCACTCG
ATTCCGTGGGCGGCGCCGCCGGCAATTCGGTGTACGAGATTGCCACCAAAAAAATCCAGCAAGAAGTCTCAACCGGCACG
GCACTCACGGTGGCCATGACCAATACCAATCTCTTTCCGTCCATGGTCTTGCAGATGTGTTCTATTGGTGAAGAATCGGG
CTCGATTGATCACATGCTCGGCAAGGCGGCAGATTTTTACGAGGCCGAGGTCGATGACATGGTGGCAGGCATCTCAAGCC
TCATGGAGCCCATCATCATTGTGATTCTGGGTGTCGTGATTGGCGGCATTGTGGTGGCCATGTACCTGCCCATTTTCAAA
CTGGGTCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21UD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.98

99.259

0.536
  pilG Neisseria gonorrhoeae MS11

53

98.765

0.523
  pilG Neisseria meningitidis 44/76-A

52.75

98.765

0.521
  pilC Legionella pneumophila strain ERS1305867

50.503

98.272

0.496
  pilC Acinetobacter baylyi ADP1

49.024

100

0.496
  pilC Acinetobacter baumannii D1279779

48.049

100

0.486
  pilC Vibrio cholerae strain A1552

40.554

98.025

0.398
  pilC Vibrio campbellii strain DS40M4

38.653

99.012

0.383
  pilC Thermus thermophilus HB27

37.221

99.506

0.37

Multiple sequence alignment