Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CS557_RS15450 Genome accession   NZ_CP024632
Coordinates   3281186..3282361 (+) Length   391 a.a.
NCBI ID   WP_099949446.1    Uniprot ID   -
Organism   Acinetobacter junii strain lzh-X15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3276186..3287361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS557_RS15425 (CS557_15430) - 3276849..3277322 (-) 474 WP_004914520.1 hypothetical protein -
  CS557_RS15430 (CS557_15435) guaD 3277508..3278833 (+) 1326 WP_099949444.1 guanine deaminase -
  CS557_RS15435 (CS557_15440) hpt 3279050..3279577 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  CS557_RS15440 (CS557_15445) - 3279807..3280058 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  CS557_RS15445 (CS557_15450) - 3280108..3280806 (-) 699 WP_099949445.1 DsbC family protein -
  CS557_RS15450 (CS557_15455) pilU 3281186..3282361 (+) 1176 WP_099949446.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CS557_RS15455 (CS557_15460) - 3282411..3283223 (-) 813 WP_099949447.1 DUF2310 family Zn-ribbon-containing protein -
  CS557_RS15460 (CS557_15465) mnmE 3283386..3284741 (-) 1356 WP_099949540.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  CS557_RS15465 (CS557_15470) yidC 3284832..3286589 (-) 1758 WP_099949448.1 membrane protein insertase YidC -
  CS557_RS15470 (CS557_15475) yidD 3286595..3286915 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  CS557_RS15475 (CS557_15480) rnpA 3286925..3287320 (-) 396 WP_099949449.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44337.86 Da        Isoelectric Point: 6.3220

>NTDB_id=254611 CS557_RS15450 WP_099949446.1 3281186..3282361(+) (pilU) [Acinetobacter junii strain lzh-X15]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDAMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=254611 CS557_RS15450 WP_099949446.1 3281186..3282361(+) (pilU) [Acinetobacter junii strain lzh-X15]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCGAGCATCAAGTTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CGGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCAATTAATGTCCCGAATGTTTCACGGTTTCGGGTCAATGTATTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GTGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGGAAATCGACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCTCTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGACAAGGTCGTCGAGCTGCGATTGAAA
TCCTCCTCAATACACCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAATTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGCGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAGGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.057

89.003

0.535
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366

Multiple sequence alignment