Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KSH_RS09795 Genome accession   NZ_CP024180
Coordinates   2204721..2206427 (-) Length   568 a.a.
NCBI ID   WP_100285198.1    Uniprot ID   -
Organism   Moraxella osloensis strain KSH     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2199721..2211427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSH_RS09775 (KSH_11040) - 2200443..2201849 (+) 1407 WP_100285195.1 nicotinate phosphoribosyltransferase -
  KSH_RS09780 (KSH_11045) coaE 2201907..2202563 (-) 657 WP_227522751.1 dephospho-CoA kinase -
  KSH_RS09785 (KSH_11050) pilD 2202560..2203435 (-) 876 WP_100285196.1 A24 family peptidase Machinery gene
  KSH_RS09790 (KSH_11055) pilC 2203441..2204664 (-) 1224 WP_100285197.1 type II secretion system F family protein Machinery gene
  KSH_RS09795 (KSH_11060) pilB 2204721..2206427 (-) 1707 WP_100285198.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KSH_RS09800 (KSH_11065) tpiA 2206846..2207685 (+) 840 WP_100285199.1 triose-phosphate isomerase -
  KSH_RS09805 (KSH_11070) secG 2207777..2208097 (+) 321 WP_065253803.1 preprotein translocase subunit SecG -
  KSH_RS09820 (KSH_11085) rimP 2208661..2209170 (+) 510 WP_007115081.1 ribosome maturation factor RimP -
  KSH_RS09825 (KSH_11090) nusA 2209288..2210769 (+) 1482 WP_007115082.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62738.28 Da        Isoelectric Point: 5.4574

>NTDB_id=252806 KSH_RS09795 WP_100285198.1 2204721..2206427(-) (pilB) [Moraxella osloensis strain KSH]
MAASLPGFGGIASRLVHEGLISEASMQKVLAESQQQKINLIAYVVDNKLAQPDAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFTTTNLADFDDMEAVE
FDETRADEDNSATSHLDINDEAPVVKFINGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKACKKPTDVPKHSLLEMGFTE
EDLADPSNVIYEPVGCSECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=252806 KSH_RS09795 WP_100285198.1 2204721..2206427(-) (pilB) [Moraxella osloensis strain KSH]
ATGGCAGCATCACTTCCTGGTTTTGGTGGCATTGCGTCACGATTAGTGCATGAAGGACTAATTAGCGAAGCGTCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAATAAATTTAATCGCCTATGTGGTTGATAATAAGCTTGCGCAACCAGACG
CGGTAGCGTGGGTGATTTCGCGAGAATTTGGCGATCCCCTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAGAAAATTATTCGCGAATTTAATATTCTGCCGATTTTTCAACGAGGTAGCCGCTTATTTGTTGCTAT
GAGTGACCCAACAAGGGTAGATGCTTTAGATGCACTGCGGTTTGGTACAAGGTTTACCGTAGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTACTACCACTAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAACCCGTGCCGATGAAGACAATAGTGCCACCAGTCATTTAGATATCAATGATGAAGCGCCTGTCGTTAAGTT
CATTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCGGACTTACATTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGCGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCAATTGATTTTCGTGTCAACTCACTGCCAACCTTATTTGGTGAAAAAATTGTACTGCGTATTTTAGATCCATCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACGGGTTCGGGTAAAACCGTCTCCTTGTACACGGGTCTTAATATCTTAAACACAGTTGA
GACCAATATTTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATTAATCAGGTCAACGTGAATAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTG
GAAACGGCTGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTACTGTCAACTTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGTAATATGGGTGTGGCATCCTTTAACATTGCCACCTCAGTCAATTTGGTAATTGCACAGCGCT
TAGCCCGTCGCCTGTGTAAAGCTTGTAAGAAACCGACAGATGTGCCAAAACATAGCTTGCTTGAAATGGGCTTTACGGAA
GAAGATTTGGCAGATCCCAGCAATGTCATTTATGAGCCCGTTGGCTGCTCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
ACGCTGCGTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTCTAAAGGTTCTAAAAGGCACGACAAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.375

99.648

0.681
  pilB Acinetobacter baylyi ADP1

65.78

99.296

0.653
  pilB Legionella pneumophila strain ERS1305867

51.06

99.648

0.509
  pilF Neisseria gonorrhoeae MS11

49.374

98.415

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

47.611

99.472

0.474
  pilB Vibrio cholerae strain A1552

51.538

91.549

0.472
  pilB Vibrio campbellii strain DS40M4

46.691

98.415

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.647

100

0.394
  pilF Thermus thermophilus HB27

36.427

100

0.366

Multiple sequence alignment