Detailed information NTG0025214

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HCH_RS28645
• Genome accession: NC_007645
• Coordinates: 6490564..6491691 (-)
• Length: 375 a.a.
• NCBI ID: WP_011400051.1
• Uniprot ID: Q2S8M7
• Organism: Hahella chejuensis KCTC 2396
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6485564..6496691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HCH_RS28625 (HCH_06347)	-	6485708..6486193 (+)	486	WP_011400047.1	fibronectin type III domain-containing protein	-
HCH_RS28630 (HCH_06348)	-	6486236..6487201 (-)	966	WP_011400048.1	homoserine kinase	-
HCH_RS28635 (HCH_06349)	-	6487286..6487564 (-)	279	WP_011400049.1	DUF2782 domain-containing protein	-
HCH_RS28640 (HCH_06350)	polA	6487752..6490487 (+)	2736	WP_011400050.1	DNA polymerase I	-
HCH_RS28645 (HCH_06351)	pilU	6490564..6491691 (-)	1128	WP_011400051.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HCH_RS28650 (HCH_06352)	pilT	6491754..6492788 (-)	1035	WP_011400052.1	type IV pilus twitching motility protein PilT	Machinery gene
HCH_RS28655 (HCH_06353)	-	6492946..6493629 (+)	684	WP_011400053.1	YggS family pyridoxal phosphate-dependent enzyme	-
HCH_RS28660 (HCH_06354)	proC	6493735..6494565 (+)	831	WP_011400054.1	pyrroline-5-carboxylate reductase	-
HCH_RS28665 (HCH_06356)	-	6494641..6495180 (+)	540	WP_011400055.1	YggT family protein	-
HCH_RS28670 (HCH_06357)	-	6495173..6495481 (+)	309	WP_011400056.1	DUF167 family protein	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 41464.61 Da
• Isoelectric Point: 6.8225

>NTDB_id=25214 HCH_RS28645 WP_011400051.1 6490564..6491691(-) (pilU) [Hahella chejuensis KCTC 2396]
MDFDKLLKIMVEKGASDLFITAGVPPSIKVHGRMVPITKSPLSPEQTRETVFSIMSEKQRADFEVGHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPKLDDLHLPEVIKSLSMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEYIHQHHGCIITQREVGLDTESFEVALKNTLRQAPDVIMIGEVRSADTMEYAVTFAETGHLCLATLHANNANQALDRI
INFFPPEQHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAIVEILINSPLAADLIRKGEVHKLKELMTKSSELGMQTFDQA
LYRAYAAGEITYEDALSHADSANDLRLMIKLGADSNISKLQGAASSLSLSDDQDY

Nucleotide

• Length: 1128 bp

>NTDB_id=25214 HCH_RS28645 WP_011400051.1 6490564..6491691(-) (pilU) [Hahella chejuensis KCTC 2396]
ATGGATTTCGACAAGCTGTTAAAAATCATGGTGGAAAAGGGGGCTTCGGACCTCTTTATCACAGCTGGGGTGCCGCCCAG
CATCAAAGTTCACGGCCGTATGGTGCCGATCACTAAGTCGCCGCTGTCTCCTGAGCAGACGCGGGAAACCGTGTTTTCCA
TTATGTCGGAAAAACAGCGTGCGGATTTTGAAGTCGGGCATGAGTGCAACTTTGCTATCAGCGCCCGCGGCATCGGTCGT
TTCCGGGTCAGCGCCTTTTACCAGCGTAACCTGTGCGGGATGGTGCTGCGTCGGATTGAGACCAATATTCCCAAGCTGGA
TGACCTGCATCTGCCGGAGGTTATCAAGTCGCTGTCGATGACCAAGCGGGGCCTGATCATCTTCGTCGGAGCGACCGGCA
CCGGTAAGTCCACGTCGCTGGCTGCAATGATCGGCCACCGGAACCGCAACAGCAAAGGGCACATCATCTCTATCGAAGAC
CCTATCGAATATATCCATCAACATCACGGCTGCATTATTACGCAGCGGGAAGTGGGGCTGGATACGGAATCGTTCGAAGT
AGCTCTGAAGAACACGCTGCGGCAGGCTCCCGACGTCATCATGATCGGGGAGGTGCGCTCCGCCGACACCATGGAATATG
CGGTGACCTTTGCGGAAACCGGTCACTTGTGTTTGGCGACATTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCAACTTTTTCCCGCCGGAGCAGCATAACCAGGTATGGATGGACTTGTCCTTGAACCTGAAAGCCATCGTGGCGCAGCA
ATTGGTGCCGACTCCAGACGGCAAGGGACGTCGGGCGATTGTTGAGATCCTCATTAACTCGCCACTGGCGGCTGACTTGA
TCCGTAAAGGGGAGGTTCACAAGCTGAAAGAGCTGATGACCAAGTCGTCGGAACTGGGTATGCAGACTTTCGATCAGGCG
CTATACCGCGCCTACGCTGCGGGAGAGATCACCTACGAAGACGCGTTGTCTCATGCGGACTCCGCGAACGATCTGCGTCT
GATGATCAAACTGGGCGCAGACAGCAATATTTCCAAGCTGCAGGGCGCCGCCAGCAGTCTGTCGTTGAGCGACGATCAGG
ATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: Q2S8M7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	76.203	99.733	0.76
pilU	Acinetobacter baylyi ADP1	64.426	95.2	0.613
pilU	Vibrio cholerae strain A1552	55.923	96.8	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	90.4	0.387
pilT	Pseudomonas aeruginosa PAK	40.708	90.4	0.368
pilT	Acinetobacter baumannii D1279779	40.118	90.4	0.363
pilT	Acinetobacter baumannii strain A118	40.118	90.4	0.363