Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BS636_RS06725 Genome accession   NZ_CP024011
Coordinates   1415207..1416916 (-) Length   569 a.a.
NCBI ID   WP_099338089.1    Uniprot ID   A0A2D1INZ5
Organism   Acinetobacter sp. LoGeW2-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1410207..1421916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS636_RS06695 (BS636_06695) - 1410291..1410614 (+) 324 WP_099338083.1 pyrimidine/purine nucleoside phosphorylase -
  BS636_RS06700 (BS636_06700) rlmB 1410768..1411517 (+) 750 WP_099338084.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BS636_RS06705 (BS636_06705) - 1411524..1412447 (+) 924 WP_099338085.1 DMT family transporter -
  BS636_RS06710 (BS636_06710) coaE 1412460..1413062 (-) 603 WP_099338086.1 dephospho-CoA kinase -
  BS636_RS16555 (BS636_06715) pilD 1413064..1413927 (-) 864 WP_099338087.1 prepilin peptidase Machinery gene
  BS636_RS06720 (BS636_06720) pilC 1413945..1415177 (-) 1233 WP_099338088.1 type II secretion system F family protein Machinery gene
  BS636_RS06725 (BS636_06725) pilB 1415207..1416916 (-) 1710 WP_099338089.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BS636_RS06730 (BS636_06730) tpiA 1417199..1417990 (+) 792 WP_099338090.1 triose-phosphate isomerase -
  BS636_RS06735 (BS636_06735) secG 1418004..1418333 (+) 330 WP_099338091.1 preprotein translocase subunit SecG -
  BS636_RS06765 (BS636_06765) rimP 1419496..1420020 (+) 525 WP_099338092.1 ribosome maturation factor RimP -
  BS636_RS06770 (BS636_06770) nusA 1420056..1421540 (+) 1485 WP_099338093.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63511.82 Da        Isoelectric Point: 4.8769

>NTDB_id=251138 BS636_RS06725 WP_099338089.1 1415207..1416916(-) (pilB) [Acinetobacter sp. LoGeW2-3]
MTAIQGSPRFTGFIRRLVDEGVISAEDMQGALTSAKKDKVDIVPYLIEHQRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNANILFIATSNPTNIEAVDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFEFDDDFD
LDVDVDTADPTQDNEDDTKADEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKPADIPRQSLLEMGFT
EQDLQDPEFKIFEPAGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=251138 BS636_RS06725 WP_099338089.1 1415207..1416916(-) (pilB) [Acinetobacter sp. LoGeW2-3]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTATTCGTCGATTGGTCGATGAAGGGGTGATATCTGCAGAAGA
TATGCAGGGTGCTTTAACTAGCGCAAAAAAAGATAAAGTCGATATTGTTCCTTATCTAATTGAACATCAGCGCCTCTCTC
CAACTAAAATTGCAGAAACCATTTCTCTTGAATTCGGTGAGCCACTATTTGATATCAGTGCCTATGATCCTGCCTTGATC
GTACGTGAAGATTTTGATGAAAAGATCATTACCAAACATCGCGTACTGCCGATCTTTAAAAATGCCAATATCCTATTTAT
CGCGACTAGTAATCCAACCAATATAGAAGCAGTTGATGCGGTCCGCTTTGCAACTAAACTCAACATTGAAACCATTATTG
TTGAACACAACAAACTCGAAAAACTGATTGAACAGAATTTTACTGAAGAAAGTACCTTTGAATTTGATGATGATTTTGAT
CTAGATGTCGATGTAGATACAGCTGATCCAACTCAAGACAATGAGGATGACACTAAAGCTGATGAAGCTCCAATCGTTAA
ATATATTAATAAATTACTGATTGATGCGATTCGTATGGGTGCATCGGACTTGCACTTTGAGCCTTATGAAAAAATGTACC
GCGTGCGTTATCGTGTCGATGGCGTACTTCGTCAAATCGCTACGCCGCCTTTACAACTGGCAACGCGTCTGGCATCCCGT
CTTAAGGTTATGTCCCAGATGGATATTTCGGAGAAACGTATTCCACAAGATGGACGTATTAAGTTAAAGCTTTCTAAAAA
TAAAGCGATTGATTTCCGTGTCAACTCGCTACCGACCCTATTCGGTGAAAAGTTAGTACTGCGTATTCTGGATCCATCCA
GTGCCATGCTAGGTATTGATGCGCTTGGCTATGAACCCGATCAAAAAGCATTGTTTATGGAAGCGCTGGATAAGCCCCAA
GGCATGCTATTGATTACTGGTCCAACAGGTTCTGGTAAAACCGTGTCTTTATATACTGGTTTAAATATCTTGAATCGTGA
AGATACCAATATTTCTACGGCTGAAGATCCAGTCGAAATTAACCTGCAAGGAATTAACCAGGTCAACGTCAACAATAAAG
TAGGTCTCACCTTCTCTGCCGCATTGAAATCTTTCCTGCGTCAGGATCCGGACATTGTCATGGTCGGCGAGATCCGTGAC
CTGGAAACAGCAGAGATTGCGATTAAGGCGGCACAAACAGGACACATGGTGATGTCAACGCTGCATACCAACAGCGCACC
TGAAACACTGACCCGTTTACGGAATATGGGTGTGCCGTCTTTTAATATTGCCACCTCGGTCAATCTGGTGATTGCCCAGC
GTCTGGCTCGTCGCCTCTGTCCTCACTGCAAAAAACCAGCAGATATTCCACGTCAGAGCCTGCTGGAAATGGGTTTTACT
GAACAGGATTTACAGGACCCGGAATTTAAGATTTTTGAACCTGCCGGATGTAATGAATGCCGGGAAGGTTATAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCGGAGATTTCACGTATTATTATGGAAGATGGCAATGCCATTGAAATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGGTCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D1INZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.298

100

0.794
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

52.222

94.903

0.496
  pilF Neisseria gonorrhoeae MS11

49.376

98.594

0.487
  pilB Vibrio cholerae strain A1552

48.677

99.649

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

97.891

0.483
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.824

100

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.138

97.012

0.36

Multiple sequence alignment