Detailed information NTG0251159

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BS636_RS16555
• Genome accession: NZ_CP024011
• Coordinates: 1413064..1413927 (-)
• Length: 287 a.a.
• NCBI ID: WP_099338087.1
• Uniprot ID: A0A2D1INZ1
• Organism: Acinetobacter sp. LoGeW2-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1408064..1418927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS636_RS06690 (BS636_06690)	recN	1408212..1409873 (-)	1662	WP_099338082.1	DNA repair protein RecN	-
BS636_RS06695 (BS636_06695)	-	1410291..1410614 (+)	324	WP_099338083.1	pyrimidine/purine nucleoside phosphorylase	-
BS636_RS06700 (BS636_06700)	rlmB	1410768..1411517 (+)	750	WP_099338084.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
BS636_RS06705 (BS636_06705)	-	1411524..1412447 (+)	924	WP_099338085.1	DMT family transporter	-
BS636_RS06710 (BS636_06710)	coaE	1412460..1413062 (-)	603	WP_099338086.1	dephospho-CoA kinase	-
BS636_RS16555 (BS636_06715)	pilD	1413064..1413927 (-)	864	WP_099338087.1	prepilin peptidase	Machinery gene
BS636_RS06720 (BS636_06720)	pilC	1413945..1415177 (-)	1233	WP_099338088.1	type II secretion system F family protein	Machinery gene
BS636_RS06725 (BS636_06725)	pilB	1415207..1416916 (-)	1710	WP_099338089.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BS636_RS06730 (BS636_06730)	tpiA	1417199..1417990 (+)	792	WP_099338090.1	triose-phosphate isomerase	-
BS636_RS06735 (BS636_06735)	secG	1418004..1418333 (+)	330	WP_099338091.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32089.43 Da
• Isoelectric Point: 8.0418

>NTDB_id=251159 BS636_RS16555 WP_099338087.1 1413064..1413927(-) (pilD) [Acinetobacter sp. LoGeW2-3]
MFSEIINYLIQTPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQLFLYPDQPIIEESKLTLSKPASTCPKCQT
PIRWYQNIPLISWLALRGKCGSCQNLISARYPFIEALTAVCALTVLAVFGPTIQMLFGLVLTYILIALTFIDFDTQLLPD
RYTLPLAALGLGINSYAIYTSPTSAIWGYIIGFLCLWIVYYLFKIITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSS
LVGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide

• Length: 864 bp

>NTDB_id=251159 BS636_RS16555 WP_099338087.1 1413064..1413927(-) (pilD) [Acinetobacter sp. LoGeW2-3]
TTGTTTAGTGAAATCATTAATTATTTAATCCAAACCCCGACTGCACTCTATATTGCAGTCGGGCTTTTTAGTCTCTGCAT
CGGTAGTTTCCTGAATGTAGTGATCTACCGCACCCCTAAAATGATGGAACAGGAATGGCGTACTGATTGCCAGTTATTTT
TATATCCTGATCAACCGATCATTGAGGAAAGCAAACTCACCTTAAGTAAGCCTGCGTCAACCTGCCCAAAATGTCAGACA
CCGATTCGCTGGTATCAGAATATTCCGTTGATCAGCTGGCTAGCTTTACGTGGAAAATGTGGTTCTTGCCAAAATCTGAT
CAGTGCCCGTTATCCATTTATTGAAGCTCTAACAGCCGTTTGTGCATTGACTGTCTTAGCTGTATTTGGTCCAACCATTC
AAATGCTGTTTGGTCTGGTACTTACTTATATCCTTATTGCACTGACCTTTATTGACTTCGACACCCAGTTATTGCCAGAC
CGCTATACCCTGCCTCTGGCAGCCTTAGGTTTGGGAATAAATAGCTATGCAATTTATACCTCACCAACGTCTGCAATTTG
GGGCTATATCATTGGCTTCTTGTGCTTATGGATCGTGTATTACCTGTTTAAAATTATTACAGGTAAAGAAGGCATGGGCT
ATGGCGACTTTAAGTTGCTGGCTGCTTTAGGTGCTTGGATGGGACCCTTAATGCTCCCTCTAATCGTGTTGCTTTCCTCA
CTCGTTGGAGCCATCATCGGTATTATCCTGCTGAAAGTACGTAAAGAAAATCAGCCTTTTGCTTTCGGTCCTTATATTGC
CATTGCAGGCTGGATCGCTTTCTTATGGGGCGAACAGATTATGAAAGTTTATTTGGGTCAATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: A0A2D1INZ1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	81.338	98.955	0.805
pilD	Acinetobacter baumannii D1279779	80.986	98.955	0.801
pilD	Vibrio cholerae strain A1552	55.805	93.031	0.519
pilD	Vibrio campbellii strain DS40M4	53.704	94.077	0.505
pilD	Neisseria gonorrhoeae MS11	46.52	95.122	0.443