Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CK910_RS13525 Genome accession   NZ_CP023818
Coordinates   2867876..2869015 (-) Length   379 a.a.
NCBI ID   WP_043139208.1    Uniprot ID   A0AAP4JAR8
Organism   Aeromonas sp. CA23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2862876..2874015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK910_RS13505 (CK910_13505) feoB 2863099..2865369 (+) 2271 WP_098983657.1 Fe(2+) transporter permease subunit FeoB -
  CK910_RS13510 (CK910_13510) - 2865366..2865608 (+) 243 WP_043140913.1 FeoC-like transcriptional regulator -
  CK910_RS13515 (CK910_13515) - 2865711..2866190 (-) 480 WP_172955311.1 WbuC family cupin fold metalloprotein -
  CK910_RS13520 (CK910_13520) glgC 2866330..2867601 (-) 1272 WP_043139205.1 glucose-1-phosphate adenylyltransferase -
  CK910_RS13525 (CK910_13525) pilU 2867876..2869015 (-) 1140 WP_043139208.1 type IVa pilus ATPase TapW Machinery gene
  CK910_RS13530 (CK910_13530) - 2869075..2869431 (-) 357 WP_042866201.1 DMT family protein -
  CK910_RS13535 (CK910_13535) - 2869545..2870276 (+) 732 WP_043556548.1 ABC transporter substrate-binding protein -
  CK910_RS13540 (CK910_13540) btuC 2870380..2871387 (+) 1008 WP_098983659.1 vitamin B12 ABC transporter permease BtuC -
  CK910_RS13545 (CK910_13545) - 2871447..2872256 (+) 810 WP_172955312.1 ATP-binding cassette domain-containing protein -
  CK910_RS13550 (CK910_13550) aac(6') 2872279..2872740 (-) 462 WP_043556544.1 aminoglycoside 6'-N-acetyltransferase -
  CK910_RS13555 (CK910_13555) - 2872751..2873815 (-) 1065 WP_043139222.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42289.45 Da        Isoelectric Point: 6.2570

>NTDB_id=250341 CK910_RS13525 WP_043139208.1 2867876..2869015(-) (pilU) [Aeromonas sp. CA23]
MELRDMLQILAQQDGSDLYLSTGAPPCAKFNGGLRPLLETPLEPGEVAKIAADIMDGEQKLQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDELF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=250341 CK910_RS13525 WP_043139208.1 2867876..2869015(-) (pilU) [Aeromonas sp. CA23]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCCAGCAGGACGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAATGGCGGATTGCGTCCCCTGCTGGAGACGCCGCTCGAGCCGGGCGAGGTGGCGAAGATTGCCGCCGACA
TCATGGACGGCGAGCAGAAGCTGCAGTTCGAACGGGAGCTGGAGATGAACCTCGCCATCTCCTTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCCCTGGTGGCCCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAACGCGGCCTTGTGCTGTTCGTCGGGGGGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATAGAGGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCGCTCAAGAATACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGCGATCGCGAAACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAGGAGCGTCGCCCGCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTCAAAACCACGGACGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCATACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATAGCCAGCAGCAGCGATGCCACGGGTTGGAGTCTGGAGCCCACCAAGGATG
AGAAAGACGAACTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.684

100

0.538
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364

Multiple sequence alignment