Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CK910_RS07360 Genome accession   NZ_CP023818
Coordinates   1573903..1575609 (-) Length   568 a.a.
NCBI ID   WP_043137334.1    Uniprot ID   -
Organism   Aeromonas sp. CA23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568903..1580609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK910_RS07330 (CK910_07330) mutT 1569498..1569905 (+) 408 WP_098981871.1 8-oxo-dGTP diphosphatase MutT -
  CK910_RS07335 (CK910_07335) yacG 1569995..1570189 (-) 195 WP_043139929.1 DNA gyrase inhibitor YacG -
  CK910_RS07340 (CK910_07340) zapD 1570199..1570921 (-) 723 WP_043139926.1 cell division protein ZapD -
  CK910_RS07345 (CK910_07345) coaE 1570959..1571573 (-) 615 WP_098981877.1 dephospho-CoA kinase -
  CK910_RS07350 (CK910_07350) pilD 1571594..1572466 (-) 873 WP_098981880.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  CK910_RS07355 (CK910_07355) pilC 1572529..1573770 (-) 1242 WP_098981882.1 type II secretion system F family protein Machinery gene
  CK910_RS07360 (CK910_07360) pilB 1573903..1575609 (-) 1707 WP_043137334.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CK910_RS07365 (CK910_07365) tapA 1575616..1576032 (-) 417 WP_098985023.1 type IVa pilus major pilin TapA -
  CK910_RS07375 (CK910_07375) nadC 1576354..1577211 (-) 858 WP_098981885.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CK910_RS07380 (CK910_07380) - 1577217..1577687 (-) 471 WP_172955263.1 TIGR02281 family clan AA aspartic protease -
  CK910_RS07385 (CK910_07385) ampD 1577836..1578408 (+) 573 WP_098981888.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CK910_RS07390 (CK910_07390) pdhR 1578685..1579449 (+) 765 WP_098981890.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62548.95 Da        Isoelectric Point: 5.4321

>NTDB_id=250332 CK910_RS07360 WP_043137334.1 1573903..1575609(-) (pilB) [Aeromonas sp. CA23]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLESDQDALGMEDIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKDCSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARIGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=250332 CK910_RS07360 WP_043137334.1 1573903..1575609(-) (pilB) [Aeromonas sp. CA23]
ATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCGTCTTCCCTGCTCAGTGAGTCCGACTCGCAGCGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTTGTGACCTTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTATACCCAGGGCCATACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTCTCGGCACTGGAGGATTTCGGCTTCAGCTTCGGACTGCATACCGAGGCGCTGCTGGTCGAAGAGAACA
AGCTGACGACCGCCATCGGCAAACTGCTGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCAGAGATC
TCCGAGCTGGAAGTTTCGGACGAAAACTCGCGACTCGATGAGAGCGTCAACACCGCCGACGACGATGCTCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGACCTGCACTTCGAACCCTATGAGACCAAATATC
GCATCCGCTTTCGGATCGACGGCATCTTGCACGAAATCGCGACACCACCGGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGGCTCGACATCGCCGAACGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCCCGCAA
CAAGTCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTCATCCGCTTGCTGGACTCCTCGG
CAGCGCGGCTCAACATAGAGCAACTCGGCTTCGACGACCGGCAGAAGGCCCAATATCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATACTGGTAACAGGCCCCACCGGCTCGGGCAAGACAGTCTCCCTCTATACCGGTCTCAATATTCTCAACACCAC
GGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGTGTCAATCAGGTACAGGTCAACCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTTCGCTCCTTTCTTCGCCAAGATCCCGACGTGGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAAACAGGCCACCTGGTGCTCTCAACTCTGCATACCAACTCGGCTGC
CGAAACCCTGACACGCATGATGAACATGGGGGTACCCGCCTTCAACATTGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCACCCGAGGTGGTACCCGAAGCCGAACTGCTGGAGCTGGGATTCACC
CAGCAGCAGCTGGCCGCCGGACTGCGCCTGTTCAAACCCGTGGGGTGCAAGGATTGCTCTGGCGGCTACAAAGGGCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGTGCCAACTCTCTACAAATTGCGG
CCATTGCTCAAAAAGAGGGGATGCGCACTCTGCGTATATCCGGTCTTGAGAAGGCTCGTATTGGAGTGACCAGCCTGGCA
GAAATCAATCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.106

100

0.551
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment