Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MKLM6_RS00975 Genome accession   NZ_CP023669
Coordinates   214796..216511 (-) Length   571 a.a.
NCBI ID   WP_064020099.1    Uniprot ID   -
Organism   Methylomonas koyamae strain LM6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 209796..221511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKLM6_RS00945 (MKLM6_0191) - 210241..211026 (-) 786 WP_064040819.1 class I SAM-dependent methyltransferase -
  MKLM6_RS00950 (MKLM6_0192) - 211049..211264 (-) 216 WP_064040818.1 DNA gyrase inhibitor YacG -
  MKLM6_RS00955 (MKLM6_0193) zapD 211264..212037 (-) 774 WP_082877665.1 cell division protein ZapD -
  MKLM6_RS00960 (MKLM6_0194) coaE 212034..212645 (-) 612 WP_096875533.1 dephospho-CoA kinase -
  MKLM6_RS00965 (MKLM6_0195) pilD 212649..213515 (-) 867 WP_096875534.1 prepilin peptidase Machinery gene
  MKLM6_RS00970 (MKLM6_0196) pilC 213576..214793 (-) 1218 WP_064020098.1 type II secretion system F family protein Machinery gene
  MKLM6_RS00975 (MKLM6_0197) pilB 214796..216511 (-) 1716 WP_064020099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKLM6_RS00980 (MKLM6_0198) thiC 216670..218538 (-) 1869 WP_064028404.1 phosphomethylpyrimidine synthase ThiC -
  MKLM6_RS00985 (MKLM6_0199) recD 218832..220472 (+) 1641 WP_064028406.1 exodeoxyribonuclease V subunit alpha -
  MKLM6_RS00990 (MKLM6_0200) - 220469..220882 (+) 414 WP_064020102.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63213.85 Da        Isoelectric Point: 6.1220

>NTDB_id=249162 MKLM6_RS00975 WP_064020099.1 214796..216511(-) (pilB) [Methylomonas koyamae strain LM6]
MATAPSNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTIATLASAEFGVPFLDLDAIDPSFL
PIKLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLMKMIDTALEAADTSMKDLLDD
DLDNIDISAGDEAKPAEETSGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKRFRIRFRADGILSEVASPPVGIANR
IISRLKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIEKLGFEPEQQKNFLDAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSVETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALKAFLRQDPDIIMVG
EIRDYETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVLLIMAQRLARRLCEHCKKEMDYPETALIS
AGFKKEDIGTFKIYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILRGGNAMEMAELSRSEGINDLRASGLLKVRQGIT
SLEEIDRVTTE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=249162 MKLM6_RS00975 WP_064020099.1 214796..216511(-) (pilB) [Methylomonas koyamae strain LM6]
ATGGCGACAGCACCTTCCAACATTCATTTCAGCGGCCTGGTTAAGTGCCTGATTCAAAAGGGGCTGCTGACCGAAGAGTC
GGCCGCGACCTGTTTGCAGGAGGCGCAAAAACGCAAACAGGCGTTTATTAGCTATCTGGTCGAACATAAATACGCAGATA
GTAAAACCATCGCCACGCTGGCCTCGGCCGAGTTCGGCGTGCCGTTCCTAGACCTGGACGCGATCGATCCGTCGTTCCTA
CCGATCAAACTGGTCAGCGAAAAATTGATTCGCCAGCACCACGCACTGCCCTTGTTCAAACGCGGCAACCGCTTGTTCGT
GGCAACGGCCGATCCGACCAATTTCCAGGCCCTGGACGAGATCAAGTTCCACACCCGGCACAACACCGAGAACATCCTGG
TCGAAGAAGACAAATTGATGAAGATGATCGATACCGCATTGGAAGCGGCCGATACATCGATGAAGGATTTGCTCGACGAC
GATCTGGACAACATCGACATTTCCGCCGGCGACGAAGCCAAGCCGGCCGAGGAAACTAGCGGTTCGGATATTGACGACGC
GCCTATCGTGCGTTTCGTCAACAAGATTCTGCTGGACTCGATCAAGCGCGGCGTATCGGATATTCACATGGAACCGTTCG
AGAAGCGGTTCCGCATCCGCTTCCGCGCCGACGGCATTCTGTCGGAAGTAGCCAGTCCGCCGGTGGGCATCGCCAACCGG
ATCATTTCCCGGCTGAAAGTCATGTCGAAAATGGACATCGCCGAACGGCGGGTGCCGCAGGACGGCCGGATCAAGATGCA
GATCTCGAAAAACCGCGCCATCGATTTCCGGGTCAATACCTGTCCGACCTTGTTCGGCGAAAAAGTGGTGTTGCGGATTC
TCGATCCGACCAGCGCCCAAATCGGTATCGAAAAACTCGGGTTCGAACCGGAACAACAGAAAAACTTCCTCGATGCGATT
CATAAACCCTACGGCATGGTATTGGTCACCGGGCCGACCGGTAGCGGTAAGACCGTGTCGCTATACACCGGTTTGAACAT
ACTGAACAGCGTCGAGACCAATATTTCCACGGCCGAAGACCCGGTAGAGATCACCGTGGAAGGCATCAACCAGGTCAACG
TCAACCCCAAAGCCGGTTTGACCTTCGCCGAAGCCCTCAAGGCGTTCTTGCGGCAAGACCCGGATATCATCATGGTCGGC
GAGATTCGCGATTACGAAACCGCCAGTATCGCGGTCAAAGCGGCGCAAACCGGCCACTTGGTGTTGTCAACGCTGCACAC
CAACGACGCGCCGCAGACCATCAACCGCTTGGTGCAAATGGGGATAGAGCCGTTCAACATCGTTTCGGCGGTTTTGCTGA
TTATGGCGCAACGGCTGGCACGGCGCTTGTGCGAGCATTGTAAAAAGGAAATGGACTACCCGGAAACTGCGCTAATCTCG
GCCGGCTTCAAAAAAGAGGACATCGGTACCTTCAAAATATATGGGCCGGTGGGCTGCGAACATTGCAATAACGGCTATAA
AGGGCGGGTCGGTATTTACCAAGTGATGCCGATCACCGAAAAAATGCGGGCCTTGATCCTGCGCGGCGGCAATGCGATGG
AAATGGCCGAATTGTCCCGCAGCGAAGGCATCAACGATTTGCGCGCCTCCGGTTTGTTGAAAGTCCGCCAGGGCATTACC
TCGCTGGAAGAAATTGACCGCGTCACCACGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.478

100

0.559
  pilB Acinetobacter baumannii D1279779

53.054

100

0.532
  pilB Acinetobacter baylyi ADP1

53.191

98.774

0.525
  pilF Neisseria gonorrhoeae MS11

47.849

97.723

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

46.085

98.424

0.454
  pilB Vibrio campbellii strain DS40M4

45.133

98.949

0.447
  pilB Vibrio cholerae strain A1552

49.409

88.967

0.44
  pilF Thermus thermophilus HB27

39.68

98.424

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

97.898

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

39.011

95.622

0.373

Multiple sequence alignment