Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MKLM6_RS00965 Genome accession   NZ_CP023669
Coordinates   212649..213515 (-) Length   288 a.a.
NCBI ID   WP_096875534.1    Uniprot ID   -
Organism   Methylomonas koyamae strain LM6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 207649..218515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKLM6_RS00935 (MKLM6_0189) argJ 208042..209256 (+) 1215 WP_096875531.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  MKLM6_RS00940 (MKLM6_0190) - 209264..210202 (+) 939 WP_096875532.1 Nudix family hydrolase -
  MKLM6_RS00945 (MKLM6_0191) - 210241..211026 (-) 786 WP_064040819.1 class I SAM-dependent methyltransferase -
  MKLM6_RS00950 (MKLM6_0192) - 211049..211264 (-) 216 WP_064040818.1 DNA gyrase inhibitor YacG -
  MKLM6_RS00955 (MKLM6_0193) zapD 211264..212037 (-) 774 WP_082877665.1 cell division protein ZapD -
  MKLM6_RS00960 (MKLM6_0194) coaE 212034..212645 (-) 612 WP_096875533.1 dephospho-CoA kinase -
  MKLM6_RS00965 (MKLM6_0195) pilD 212649..213515 (-) 867 WP_096875534.1 prepilin peptidase Machinery gene
  MKLM6_RS00970 (MKLM6_0196) pilC 213576..214793 (-) 1218 WP_064020098.1 type II secretion system F family protein Machinery gene
  MKLM6_RS00975 (MKLM6_0197) pilB 214796..216511 (-) 1716 WP_064020099.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31600.62 Da        Isoelectric Point: 7.4393

>NTDB_id=249160 MKLM6_RS00965 WP_096875534.1 212649..213515(-) (pilD) [Methylomonas koyamae strain LM6]
MLFNALAQSSGFVTGSALVLGLLVGSFLNVVIYRLPLMMQAGWRRECREYLELPAEAPAERFDLLLPASHCPACKAEIKA
YQNIPVFSYLLLGGKCANCGTKIAWRYPLIEAFTGLCSALVAWQLGYGWALAFALPLTWCLIALSFIDIDQQLLPDSITL
PLVWLGLLLSVFGIFADSRDSIIGAVAGYLSLWCVFHGFKLLTGKEGMGYGDFKLLAVFGAWLGWQYLPLIVLLSSLVGA
TVGIAMIVFCRRDAAKPIPFGPYLAMAGWLALLWGEQMNAWYLRITGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=249160 MKLM6_RS00965 WP_096875534.1 212649..213515(-) (pilD) [Methylomonas koyamae strain LM6]
ATGTTGTTTAACGCCTTAGCCCAATCCTCCGGTTTTGTGACCGGCAGCGCTTTAGTCCTCGGCCTGTTGGTCGGCAGCTT
TCTGAACGTGGTGATTTACCGGCTGCCGCTGATGATGCAGGCCGGTTGGCGCCGAGAGTGCCGCGAGTACCTGGAATTGC
CGGCCGAAGCTCCGGCCGAACGTTTCGACTTGTTGCTGCCGGCTTCGCATTGCCCGGCTTGTAAAGCGGAAATCAAAGCC
TACCAAAACATCCCGGTCTTCAGTTACCTGCTGCTGGGCGGCAAATGCGCCAACTGCGGCACGAAAATCGCTTGGCGCTA
CCCGCTGATCGAGGCCTTTACCGGTCTGTGTTCGGCGCTGGTGGCCTGGCAACTGGGTTACGGTTGGGCCTTGGCATTTG
CCTTGCCGTTGACTTGGTGCCTGATTGCGTTGAGTTTTATCGACATCGACCAACAATTGTTGCCCGATTCCATCACGCTG
CCCTTGGTGTGGTTGGGCTTGCTGCTCAGCGTATTCGGCATTTTTGCCGACAGCCGCGACAGTATTATCGGTGCGGTAGC
CGGCTATTTGAGCCTCTGGTGCGTATTCCACGGCTTCAAATTGCTGACCGGCAAGGAAGGCATGGGCTACGGCGATTTCA
AATTGCTGGCCGTATTCGGCGCTTGGCTGGGCTGGCAATATCTGCCTTTGATCGTATTGTTGTCTTCCCTGGTCGGCGCA
ACGGTCGGCATAGCGATGATCGTGTTCTGCCGGCGCGATGCGGCCAAGCCGATCCCGTTCGGGCCTTACTTAGCAATGGC
CGGCTGGCTGGCCTTGCTGTGGGGCGAGCAAATGAATGCCTGGTATCTGCGAATTACGGGCCTGTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

54.275

93.403

0.507
  pilD Acinetobacter baumannii D1279779

53.358

93.056

0.497
  pilD Acinetobacter nosocomialis M2

52.612

93.056

0.49
  pilD Neisseria gonorrhoeae MS11

51.866

93.056

0.483
  pilD Vibrio cholerae strain A1552

51.493

93.056

0.479

Multiple sequence alignment