Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IS_RS18030 Genome accession   NC_007508
Coordinates   3838617..3840326 (-) Length   569 a.a.
NCBI ID   WP_039416953.1    Uniprot ID   A0AAQ0YN81
Organism   Xanthomonas euvesicatoria pv. vesicatoria str. 85-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3833617..3845326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS_RS18010 (XCV3349) sucC 3833667..3834836 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  IS_RS18015 (XCV3350) - 3835068..3836681 (+) 1614 WP_011348336.1 sensor histidine kinase -
  IS_RS18025 (XCV3351) pilR 3836948..3838402 (+) 1455 WP_011348337.1 sigma-54-dependent transcriptional regulator Regulator
  IS_RS18030 (XCV3352) pilB 3838617..3840326 (-) 1710 WP_039416953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IS_RS18035 (XCV3353) pilA 3840484..3840912 (-) 429 WP_011348339.1 pilin Machinery gene
  IS_RS18040 (XCV3354) pilC 3841254..3842516 (+) 1263 WP_011348340.1 type II secretion system F family protein Machinery gene
  IS_RS18045 (XCV3355) - 3842523..3843386 (+) 864 WP_008576868.1 prepilin peptidase -
  IS_RS18050 (XCV3356) coaE 3843400..3844011 (+) 612 WP_011348341.1 dephospho-CoA kinase -
  IS_RS26130 (XCV3357) - 3844193..3845233 (+) 1041 WP_011348342.1 RHS repeat domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61684.59 Da        Isoelectric Point: 5.4503

>NTDB_id=24899 IS_RS18030 WP_039416953.1 3838617..3840326(-) (pilB) [Xanthomonas euvesicatoria pv. vesicatoria str. 85-10]
MGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAAQGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=24899 IS_RS18030 WP_039416953.1 3838617..3840326(-) (pilB) [Xanthomonas euvesicatoria pv. vesicatoria str. 85-10]
GTGGGAATTACAGGCATTGCACGTCGTTTGGTGCAGGATGGAGCACTGGATGAGGCAAGTGCCCGTACCGCAATGGACCA
AGCCGCGGCGGCGAAGGTTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTTCAGCGTCTCAATTGGCCGCTGCCA
ACGCAGTCGAGTTCGGCATGCCGCTGCTCGATGTTTCTGCATTCGACGCCAGCCAGAACGCAGTAAAGCTCGTCAGTGAG
GAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAATCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAAATGGGGGATCTG
GACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAACCAGCGCATCGCAGCG
CGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGAGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTGGAAGCGATCCACAAGCCC
TATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTTGTACACGGCGCTGGGCATCCTCAACGA
CGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTGGCGTCAATCAGGTACAGCAGAACAACA
AGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGT
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTTGTCGACGTTGCACACCAACGATGC
GCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTACCTCGTCGGTGACCTTGGTGATCGCGC
AACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCTGACAACGCCTTGCTGGCCGAAGGATTC
ACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGAGTGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAGGGCGGCAATGCGATGCAGATTG
CCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATGAAGGCTGCGCAGGGGGTGACCAGCCTG
GCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.042

99.824

0.569
  pilB Acinetobacter baylyi ADP1

55.81

99.824

0.557
  pilB Legionella pneumophila strain ERS1305867

53.076

100

0.531
  pilF Neisseria gonorrhoeae MS11

50.176

99.824

0.501
  pilB Vibrio cholerae strain A1552

49.04

100

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.97

0.367
  pilF Thermus thermophilus HB27

39.808

91.388

0.364

Multiple sequence alignment