Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CPA52_RS02045 Genome accession   NZ_CP023558
Coordinates   448431..449660 (-) Length   409 a.a.
NCBI ID   WP_039038010.1    Uniprot ID   -
Organism   Pseudoalteromonas marina strain ECSMB14103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443431..454660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPA52_RS02020 (CPA52_02020) - 444063..444554 (+) 492 WP_039038007.1 Spy/CpxP family protein refolding chaperone -
  CPA52_RS02025 (CPA52_02025) yacG 444636..444866 (-) 231 WP_006794561.1 DNA gyrase inhibitor YacG -
  CPA52_RS02030 (CPA52_02030) pilB 444934..446610 (-) 1677 WP_008131082.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CPA52_RS02035 (CPA52_02035) coaE 446743..447375 (-) 633 WP_039038008.1 dephospho-CoA kinase -
  CPA52_RS02040 (CPA52_02040) pilD 447393..448286 (-) 894 WP_039038009.1 A24 family peptidase Machinery gene
  CPA52_RS02045 (CPA52_02045) pilC 448431..449660 (-) 1230 WP_039038010.1 type II secretion system F family protein Machinery gene
  CPA52_RS02050 (CPA52_02050) - 449749..451092 (-) 1344 WP_039038011.1 O-antigen ligase family protein -
  CPA52_RS02055 (CPA52_02055) - 451153..451575 (-) 423 WP_039038012.1 pilin -
  CPA52_RS02060 (CPA52_02060) nadC 452102..452947 (-) 846 WP_039038013.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CPA52_RS02065 (CPA52_02065) ampD 453164..453718 (+) 555 WP_039038014.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CPA52_RS02070 (CPA52_02070) ampE 453732..454583 (+) 852 WP_008131099.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44333.35 Da        Isoelectric Point: 9.8363

>NTDB_id=248369 CPA52_RS02045 WP_039038010.1 448431..449660(-) (pilC) [Pseudoalteromonas marina strain ECSMB14103]
MAKKQQDKGLDTFQWVGVSARGKKLEGELTGNSIALVKAQLRKQGITPSKVKRKPKPLFGIQSVQKITPKDIALVTRQIA
TMLMAGVPLIQAIEMIGTGSTNKSVGKLMETIGDEVKAGQPLSQALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKSEALKSKIKKAMFYPISVLVIAGIVTSILLIFVVPQFQDIFSGFGAELPAFTLFVIGISEFMQAYWWMILIAIVGFG
YAFKEAKLRSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=248369 CPA52_RS02045 WP_039038010.1 448431..449660(-) (pilC) [Pseudoalteromonas marina strain ECSMB14103]
ATGGCAAAAAAACAACAAGATAAAGGCCTCGATACATTTCAGTGGGTCGGCGTAAGTGCCCGAGGGAAAAAACTTGAAGG
AGAGCTTACAGGGAATAGCATTGCACTTGTTAAAGCTCAGCTAAGAAAGCAAGGCATTACACCTTCAAAAGTAAAGCGTA
AGCCAAAACCTTTATTTGGCATTCAAAGTGTTCAAAAAATCACCCCTAAAGATATAGCGCTTGTTACACGCCAAATCGCC
ACCATGCTTATGGCGGGTGTACCTCTTATACAAGCCATTGAAATGATAGGCACGGGCTCAACTAATAAAAGCGTGGGTAA
ACTAATGGAAACCATTGGCGATGAAGTAAAGGCAGGTCAGCCTTTATCGCAAGCGCTAAGAAAACACCCTCGTTATTTTG
ATGACTTATATTGCGACTTAGTCGCATCGGGCGAGCAATCAGGTGCGCTTGATAAAATATTTGATCGTGTTGCATTGTAC
AAAGAAAAATCTGAAGCGCTTAAATCAAAAATAAAAAAAGCAATGTTTTACCCAATATCTGTATTAGTGATAGCAGGTAT
TGTAACTTCTATTTTGCTAATCTTCGTAGTGCCACAATTTCAGGATATATTCTCTGGCTTTGGCGCTGAACTCCCAGCAT
TTACTTTATTTGTAATCGGGATATCTGAGTTTATGCAAGCGTATTGGTGGATGATTTTAATTGCAATTGTTGGCTTTGGT
TATGCGTTTAAAGAAGCCAAACTCAGAAGCTTAAAACTCAGAGATGCAACCGATAGAGCCATTTTAAAGCTCCCTGTGAT
TGGAATGATTTTAAATAAAGCAGCTGTTGCACGCTATGCCCGAACACTTTCCACCACTTTTGCAGCCGGTGTACCACTTG
TTGATGCGCTTGACTCTGCTGCAGGTGCTTCGGGTAACGCAGTGTACCGTTATGCTATTTTAGATATTAAAGCTGAGGTT
AGCTCGGGTAATCAAATGAACTGGGCCATGCGTAACTCTAAAATATTCCCTGATATGGTAATTCAAATGGTTGCCATTGG
TGAAGAGTCTGGCTCGTTAGATGGCATGCTGGCAAAAGTAGCGACTATTTACGAGCAAGAGGTTGATGATGCCGTCGATG
GCCTTTCAAGCCTGTTAGAACCACTTATTATGGCGGTACTTGGTGTGCTCGTAGGCGGCTTAATTGTTGCCATGTACTTA
CCTATTTTCCAATTAGGTTCTGTCATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

63.476

97.066

0.616
  pilC Acinetobacter baylyi ADP1

55.746

100

0.557
  pilC Legionella pneumophila strain ERS1305867

56.5

97.8

0.553
  pilC Acinetobacter baumannii D1279779

55.5

97.8

0.543
  pilC Vibrio cholerae strain A1552

44.472

99.511

0.443
  pilG Neisseria meningitidis 44/76-A

44.898

95.844

0.43
  pilG Neisseria gonorrhoeae MS11

44.643

95.844

0.428
  pilC Vibrio campbellii strain DS40M4

43

97.8

0.421
  pilC Thermus thermophilus HB27

36.908

98.044

0.362

Multiple sequence alignment