Detailed information NTG0024821

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: NOC_RS13515
• Genome accession: NC_007484
• Coordinates: 2890339..2890782 (-)
• Length: 147 a.a.
• NCBI ID: WP_004269175.1
• Uniprot ID: Q3J853
• Organism: Nitrosococcus oceani ATCC 19707
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2885339..2895782

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NOC_RS13480 (Noc_2533)	-	2885728..2887071 (+)	1344	WP_004269172.1	MATE family efflux transporter	-
NOC_RS13485 (Noc_2534)	-	2887081..2887509 (-)	429	WP_004164010.1	YqaA family protein	-
NOC_RS13490 (Noc_2535)	-	2887957..2888307 (+)	351	WP_004269146.1	nucleotidyltransferase family protein	-
NOC_RS13495 (Noc_2536)	-	2888295..2888711 (+)	417	WP_004269203.1	nucleotidyltransferase substrate binding protein	-
NOC_RS18500	-	2888860..2888943 (+)	84	WP_342341972.1	type II toxin-antitoxin system HicA family toxin	-
NOC_RS13500 (Noc_2537)	-	2888947..2889345 (+)	399	WP_004269179.1	type II toxin-antitoxin system HicB family antitoxin	-
NOC_RS13505 (Noc_2538)	-	2889675..2889944 (+)	270	WP_004269188.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
NOC_RS13510 (Noc_2539)	-	2889941..2890150 (+)	210	WP_011330994.1	hypothetical protein	-
NOC_RS13515 (Noc_2540)	pilA2	2890339..2890782 (-)	444	WP_004269175.1	pilin	Machinery gene
NOC_RS13525 (Noc_2541)	-	2891198..2891437 (+)	240	WP_004269108.1	ribbon-helix-helix domain-containing protein	-
NOC_RS13530 (Noc_2542)	-	2891872..2892369 (-)	498	WP_011330995.1	helix-turn-helix domain-containing protein	-
NOC_RS13535 (Noc_2543)	-	2892366..2893643 (-)	1278	WP_004269107.1	RNA-guided endonuclease InsQ/TnpB family protein	-
NOC_RS13540 (Noc_2544)	-	2893878..2894288 (-)	411	WP_004164036.1	type II toxin-antitoxin system VapC family toxin	-
NOC_RS13545 (Noc_2545)	-	2894285..2894554 (-)	270	WP_011330997.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
NOC_RS13550 (Noc_2546)	-	2894664..2895230 (-)	567	WP_011330998.1	Uma2 family endonuclease	-

Sequence

Protein

• Length: 147 a.a.
• Molecular weight: 15310.44 Da
• Isoelectric Point: 4.7671

>NTDB_id=24821 NOC_RS13515 WP_004269175.1 2890339..2890782(-) (pilA2) [Nitrosococcus oceani ATCC 19707]
MRKVQQGFTLIELMIVVAIIGILASVAIPAYQDYTIRAKVSEAVTLASEAKTAVAETAASLGGLTNVTAANSGYELSNKT
DYVDSIIIADDGVITVTTKATGASTQPVFVLKPTQTNSEDPITWVCTTSAGELKHVPASCRTATTTS

Nucleotide

• Length: 444 bp

>NTDB_id=24821 NOC_RS13515 WP_004269175.1 2890339..2890782(-) (pilA2) [Nitrosococcus oceani ATCC 19707]
ATGAGAAAAGTACAACAGGGTTTTACCTTGATTGAATTGATGATCGTGGTGGCGATCATTGGTATTTTGGCTTCTGTGGC
CATCCCCGCCTATCAGGACTACACCATTCGGGCAAAGGTATCTGAAGCGGTAACACTTGCTTCTGAAGCTAAAACGGCGG
TTGCTGAAACTGCAGCATCACTGGGGGGGTTAACGAACGTTACTGCAGCCAACTCCGGTTATGAGCTTTCTAATAAGACT
GATTATGTAGATAGTATTATAATTGCAGATGATGGTGTCATTACTGTTACTACGAAAGCGACGGGTGCTTCTACCCAGCC
TGTATTTGTTCTAAAACCAACGCAAACAAACTCAGAAGATCCAATTACCTGGGTTTGTACAACGTCTGCTGGGGAGTTGA
AGCACGTACCGGCCTCATGTCGTACCGCTACCACAACTTCTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (2-27)

• Pilin (bacterial filament) (Pilin) (37-141)

3D structure

• AlphaFold DB: Q3J853

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	54.93	96.599	0.531
pilA2	Legionella pneumophila strain ERS1305867	54.225	96.599	0.524
pilA	Ralstonia pseudosolanacearum GMI1000	40.476	100	0.463
comP	Acinetobacter baylyi ADP1	45.333	100	0.463
pilE	Neisseria gonorrhoeae strain FA1090	37.736	100	0.408
pilE	Neisseria gonorrhoeae MS11	36.25	100	0.395
pilA/pilA1	Eikenella corrodens VA1	37.5	100	0.388
pilA/pilAI	Pseudomonas stutzeri DSM 10701	38.571	95.238	0.367