Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CMV05_RS02540 Genome accession   NZ_CP023310
Coordinates   510892..512580 (-) Length   562 a.a.
NCBI ID   WP_096035998.1    Uniprot ID   -
Organism   Vibrio anguillarum strain VIB12 isolate Sea bass     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505892..517580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV05_RS02510 (CMV05_02510) rplS 506610..506963 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  CMV05_RS02515 (CMV05_02515) yacG 507044..507241 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  CMV05_RS02520 (CMV05_02520) zapD 507331..508071 (-) 741 WP_013857689.1 cell division protein ZapD -
  CMV05_RS02525 (CMV05_02525) coaE 508104..508712 (-) 609 WP_096035996.1 dephospho-CoA kinase -
  CMV05_RS02530 (CMV05_02530) pilD 508703..509584 (-) 882 WP_088720881.1 A24 family peptidase Machinery gene
  CMV05_RS02535 (CMV05_02535) pilC 509623..510849 (-) 1227 WP_096035997.1 type II secretion system F family protein Machinery gene
  CMV05_RS02540 (CMV05_02540) pilB 510892..512580 (-) 1689 WP_096035998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CMV05_RS02545 (CMV05_02545) - 512585..513040 (-) 456 WP_176428646.1 pilin -
  CMV05_RS02550 (CMV05_02550) nadC 513300..514187 (-) 888 WP_017047002.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CMV05_RS02555 (CMV05_02555) ampD 514312..514863 (+) 552 WP_234913521.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CMV05_RS02560 (CMV05_02560) fldB 514885..515403 (-) 519 WP_013857680.1 flavodoxin FldB -
  CMV05_RS02565 (CMV05_02565) xerD 515556..516464 (+) 909 WP_096036001.1 site-specific tyrosine recombinase XerD -
  CMV05_RS02570 (CMV05_02570) - 516478..517251 (+) 774 WP_096036002.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62261.38 Da        Isoelectric Point: 6.3250

>NTDB_id=245862 CMV05_RS02540 WP_096035998.1 510892..512580(-) (pilB) [Vibrio anguillarum strain VIB12 isolate Sea bass]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPLLCQQLGLRD
LITRYQALPIAHNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVEPSIQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=245862 CMV05_RS02540 WP_096035998.1 510892..512580(-) (pilB) [Vibrio anguillarum strain VIB12 isolate Sea bass]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCAAGCGTGCCAGAAGCTTTGCTCGCATTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACTGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCCATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTCGCAACAGGCTTACAAGTTGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCACCAGTCAGCCGTTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGATTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTGCTACGTCTACTCGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAGCTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGTCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCTACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGCATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATCGCCGTGAAAGCAGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTGGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTTGAGCCCTCGATACAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTAACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.274

100

0.833
  pilB Vibrio campbellii strain DS40M4

74.733

100

0.747
  pilB Vibrio parahaemolyticus RIMD 2210633

73.31

100

0.733
  pilB Acinetobacter baumannii D1279779

51.731

92.527

0.479
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.965

92.171

0.47
  pilF Neisseria gonorrhoeae MS11

44.425

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

36.149

100

0.381
  pilF Thermus thermophilus HB27

37.213

100

0.375

Multiple sequence alignment