Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CKY39_RS06975 Genome accession   NZ_CP023284
Coordinates   1552314..1553543 (-) Length   409 a.a.
NCBI ID   WP_095743912.1    Uniprot ID   A0A250DG80
Organism   Variovorax boronicumulans strain J1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1547314..1558543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKY39_RS06950 (CKY39_06950) zapD 1547324..1548079 (-) 756 WP_070063618.1 cell division protein ZapD -
  CKY39_RS06955 (CKY39_06955) - 1548116..1549147 (-) 1032 WP_062474527.1 hypothetical protein -
  CKY39_RS06960 (CKY39_06960) - 1549154..1550647 (-) 1494 WP_095743910.1 MBOAT family O-acyltransferase -
  CKY39_RS06965 (CKY39_06965) coaE 1550724..1551335 (-) 612 WP_167394982.1 dephospho-CoA kinase -
  CKY39_RS06970 (CKY39_06970) - 1551349..1552314 (-) 966 WP_095743911.1 prepilin peptidase -
  CKY39_RS06975 (CKY39_06975) pilC 1552314..1553543 (-) 1230 WP_095743912.1 type II secretion system F family protein Machinery gene
  CKY39_RS06980 (CKY39_06980) pilB 1553568..1555301 (-) 1734 WP_062474518.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKY39_RS06990 (CKY39_06990) - 1555546..1556538 (-) 993 WP_070063612.1 polyprenyl synthetase family protein -
  CKY39_RS06995 (CKY39_06995) rplU 1556721..1557032 (+) 312 WP_042578903.1 50S ribosomal protein L21 -
  CKY39_RS07000 (CKY39_07000) rpmA 1557045..1557305 (+) 261 WP_013543198.1 50S ribosomal protein L27 -
  CKY39_RS07005 (CKY39_07005) cgtA 1557378..1558454 (+) 1077 WP_062474512.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44750.49 Da        Isoelectric Point: 9.6713

>NTDB_id=245724 CKY39_RS06975 WP_095743912.1 1552314..1553543(-) (pilC) [Variovorax boronicumulans strain J1]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRIPVFGTLIEKSCIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=245724 CKY39_RS06975 WP_095743912.1 1552314..1553543(-) (pilC) [Variovorax boronicumulans strain J1]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACGCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGTGAACTTCGCGCCGCCGGGGAGAACCAGGTGCAGGCGGCCTTGCGTCGCCAGGGCGTACTGGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAACTCGCG
ACCATGATGAAGGCCGGGGTGCCGCTGCTGCAGTCCTTCGACATCGTGGGCCGCGGCAACGCGAACCCGAGCGTCGCCAA
GCTGCTGAACGACATCCGCAGCGATGTCGAGACCGGCACCTCGCTTTCGGCCGCTTTTCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTATTGCAACCTGGTGGAAGCCGGTGAGGCGGCCGGTATTCTGGAGGACCTGCTCGACCGCTTGGCGACCTAC
ATGGAAAAGACCGAAGCGATCAAGTCCAAGATCAAGTCGGCATTGATGTACCCGACCTCGGTGGTCGTCGTGGCCTTCGT
GGTGGTGGCGATCATCATGATCTTCGTGATCCCTGCCTTCAAGCAGGTGTTCACCTCGTTCGGAGCCGACCTGCCCGCGC
CTACGCTCTTCGTGATGGCAATGAGCGAGTTCTTCGTTTCCTATTGGTGGCTGATCTTCGGCGTGCTCGGTGGTGGCATC
TATTTCTTTCTGCAAGCGTGGAAACGCAATGAGCGCGTCCAGAAGGTCATGGACCGCCTGCTGCTGCGCATTCCCGTCTT
CGGCACGCTGATCGAGAAATCCTGCATCGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCGGCCGGCGTGCCGCTGG
TGGAGGCGCTCGACTCCGTGGGCGGTGCATCGGGCAACTCGGTCTACGGCGATGCCACCGCCAAGATCCAGCAGGAGGTC
TCGACCGGCACCAGCCTCACGACGGCCATGACCAACGTCAACCTGTTCCCGTCGATGGTGATCCAGATGACTGCCATCGG
CGAGGAGTCGGGCTCGATCGACCACATGCTCGGCAAGGCCGCTGACTTCTACGAGTCTGAAGTCGACGACATGGTCGCCG
GCCTCTCCAGCCTGATGGAGCCCATCATCATCGTGTTCCTGGGCGTCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A250DG80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

98.778

0.531
  pilG Neisseria gonorrhoeae MS11

52.897

97.066

0.513
  pilC Acinetobacter baylyi ADP1

51.095

100

0.513
  pilG Neisseria meningitidis 44/76-A

52.645

97.066

0.511
  pilC Legionella pneumophila strain ERS1305867

50.505

96.822

0.489
  pilC Acinetobacter baumannii D1279779

50.126

97.066

0.487
  pilC Vibrio cholerae strain A1552

40.955

97.311

0.399
  pilC Thermus thermophilus HB27

38.663

100

0.396
  pilC Vibrio campbellii strain DS40M4

39.055

98.289

0.384

Multiple sequence alignment