Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CLM75_RS04065 Genome accession   NZ_CP023272
Coordinates   894548..895756 (-) Length   402 a.a.
NCBI ID   WP_105161637.1    Uniprot ID   -
Organism   Pseudomonas lurida strain MYb11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 889548..900756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM75_RS04025 (CLM75_03995) - 890466..890639 (-) 174 WP_016976295.1 DUF3094 family protein -
  CLM75_RS04030 (CLM75_04000) - 890694..891323 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  CLM75_RS04035 (CLM75_04005) - 891427..891894 (+) 468 WP_105161636.1 FAD/FMN-containing dehydrogenase -
  CLM75_RS04040 (CLM75_04010) - 891891..892580 (+) 690 WP_034102090.1 energy-coupling factor ABC transporter permease -
  CLM75_RS04045 (CLM75_04015) - 892656..892871 (+) 216 WP_003171680.1 hypothetical protein -
  CLM75_RS04050 (CLM75_04020) yacG 892868..893068 (-) 201 WP_034102092.1 DNA gyrase inhibitor YacG -
  CLM75_RS04055 (CLM75_04025) coaE 893065..893688 (-) 624 WP_069021704.1 dephospho-CoA kinase -
  CLM75_RS04060 (CLM75_04030) pilD 893685..894551 (-) 867 WP_098465764.1 prepilin peptidase Machinery gene
  CLM75_RS04065 (CLM75_04035) pilC 894548..895756 (-) 1209 WP_105161637.1 type II secretion system F family protein Machinery gene
  CLM75_RS04070 (CLM75_04040) - 895959..896372 (+) 414 WP_034102485.1 pilin -
  CLM75_RS04075 (CLM75_04045) - 896408..897808 (+) 1401 WP_105161638.1 O-antigen ligase family protein -
  CLM75_RS04085 (CLM75_04055) - 898089..898394 (+) 306 WP_105161639.1 DUF6388 family protein -
  CLM75_RS04090 (CLM75_04060) nadC 898469..899317 (-) 849 WP_034102102.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43282.71 Da        Isoelectric Point: 10.2543

>NTDB_id=245663 CLM75_RS04065 WP_105161637.1 894548..895756(-) (pilC) [Pseudomonas lurida strain MYb11]
MDNASTLYAWEGINRKGRRVSGQTTGHSVARVKAQLRQQGISPGHVRKKSPVLPSFVPSIKPADITLFTRQLATLLKAGI
PLLQAFDVIGEGFDNRPMRKLVQGLKQAIAAGTSLAEALRKQPHYFDALYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPITVLVVASLVTGVLLVHVVPQFQTLFAGVDGKLPGFTLQVIALSEFMQKAWWIIALGILAAGLGLRHAY
RTFADVRAWLDSCLLKTPLAGKLLSKSAVARYARTLSTTFAAGVPLVQALDSVAGAAGSGPFRQAIEHMRRDVATGMQLN
QSMVASGLFPRMAIQMTAIGEESGTLDSMLEKVASHYEADVDNVVDNLTNLMEPLIMVVLGGIVGALVVAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=245663 CLM75_RS04065 WP_105161637.1 894548..895756(-) (pilC) [Pseudomonas lurida strain MYb11]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAGACCACCGGGCA
CAGCGTTGCAAGGGTCAAGGCTCAGCTACGCCAACAGGGCATCAGCCCTGGGCATGTGCGCAAGAAATCCCCAGTGCTGC
CGAGCTTCGTACCCTCGATCAAGCCGGCGGACATCACCCTGTTCACGCGCCAACTGGCTACGCTGCTCAAGGCCGGGATT
CCTCTCCTGCAAGCCTTCGACGTCATCGGTGAAGGTTTCGACAACCGGCCCATGCGTAAGCTGGTGCAAGGGTTGAAACA
GGCGATTGCCGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAACCTCATTATTTCGACGCGCTGTACTGCAACCTGG
TAGCCGCCGGCGAACAGGCCGGAGCCCTTGAAACCTTGCTGGAACGCGTGGCAATCCACCGAGAGAAGAGCGAACAGCTA
AAGGCCAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGTCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGACGCTGTTCGCCGGCGTCGACGGCAAGCTGCCTGGCTTCACATTGCAGGTGATCGCCC
TTTCCGAATTCATGCAGAAAGCCTGGTGGATCATCGCGCTGGGCATTCTTGCGGCAGGCTTGGGGTTGCGCCACGCCTAC
CGTACCTTCGCCGATGTTCGTGCCTGGCTGGACAGCTGTTTGTTGAAAACGCCCCTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTACGCCCGCACGCTTTCCACCACGTTTGCCGCCGGCGTTCCGTTGGTGCAGGCGCTGGACTCGGTGG
CCGGGGCCGCCGGTTCAGGGCCGTTCAGACAGGCAATCGAACATATGCGACGCGACGTAGCGACGGGCATGCAATTGAAT
CAATCCATGGTCGCCAGCGGCCTGTTTCCCCGCATGGCGATCCAGATGACAGCCATCGGCGAAGAATCAGGCACGCTGGA
CAGCATGCTGGAAAAGGTCGCCAGCCATTACGAGGCCGATGTGGATAACGTGGTCGATAACCTCACCAACCTTATGGAGC
CGCTGATTATGGTGGTACTGGGAGGGATCGTCGGCGCACTGGTGGTCGCGATGTATTTACCGGTATTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.848

98.507

0.59
  pilC Acinetobacter baumannii D1279779

51.392

98.259

0.505
  pilC Acinetobacter baylyi ADP1

51.01

98.507

0.502
  pilC Legionella pneumophila strain ERS1305867

49.114

98.259

0.483
  pilC Vibrio cholerae strain A1552

41.919

98.507

0.413
  pilC Vibrio campbellii strain DS40M4

40.05

98.756

0.396
  pilG Neisseria gonorrhoeae MS11

41.253

95.274

0.393
  pilG Neisseria meningitidis 44/76-A

39.583

95.522

0.378

Multiple sequence alignment