Detailed information NTG0245662

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CLM75_RS04060
• Genome accession: NZ_CP023272
• Coordinates: 893685..894551 (-)
• Length: 288 a.a.
• NCBI ID: WP_098465764.1
• Uniprot ID: -
• Organism: Pseudomonas lurida strain MYb11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 888685..899551

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CLM75_RS04020 (CLM75_03990)	-	889107..890405 (-)	1299	WP_034102086.1	NAD(P)/FAD-dependent oxidoreductase	-
CLM75_RS04025 (CLM75_03995)	-	890466..890639 (-)	174	WP_016976295.1	DUF3094 family protein	-
CLM75_RS04030 (CLM75_04000)	-	890694..891323 (-)	630	WP_010213242.1	DUF1780 domain-containing protein	-
CLM75_RS04035 (CLM75_04005)	-	891427..891894 (+)	468	WP_105161636.1	FAD/FMN-containing dehydrogenase	-
CLM75_RS04040 (CLM75_04010)	-	891891..892580 (+)	690	WP_034102090.1	energy-coupling factor ABC transporter permease	-
CLM75_RS04045 (CLM75_04015)	-	892656..892871 (+)	216	WP_003171680.1	hypothetical protein	-
CLM75_RS04050 (CLM75_04020)	yacG	892868..893068 (-)	201	WP_034102092.1	DNA gyrase inhibitor YacG	-
CLM75_RS04055 (CLM75_04025)	coaE	893065..893688 (-)	624	WP_069021704.1	dephospho-CoA kinase	-
CLM75_RS04060 (CLM75_04030)	pilD	893685..894551 (-)	867	WP_098465764.1	prepilin peptidase	Machinery gene
CLM75_RS04065 (CLM75_04035)	pilC	894548..895756 (-)	1209	WP_105161637.1	type II secretion system F family protein	Machinery gene
CLM75_RS04070 (CLM75_04040)	-	895959..896372 (+)	414	WP_034102485.1	pilin	-
CLM75_RS04075 (CLM75_04045)	-	896408..897808 (+)	1401	WP_105161638.1	O-antigen ligase family protein	-
CLM75_RS04085 (CLM75_04055)	-	898089..898394 (+)	306	WP_105161639.1	DUF6388 family protein	-
CLM75_RS04090 (CLM75_04060)	nadC	898469..899317 (-)	849	WP_034102102.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31698.89 Da
• Isoelectric Point: 8.4958

>NTDB_id=245662 CLM75_RS04060 WP_098465764.1 893685..894551(-) (pilD) [Pseudomonas lurida strain MYb11]
MSLLLAGHPWAFIALASVLGLIVGSFLNVLVWRLPKMLEREWRAQAHEVLGLPAEPAGPTYNLMHPNSCCPQCNHPIRPR
ENIPLLSYLFLRGRCAQCQKAISVRYPLTELACGLISAWVAWHFGFGWPAGAVLVLSWGLLAMSLIDADHQLLPDVLVMP
LLWLGLIVNGFGLLTTLPDALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMSSLVGVF
VGLILMRLRKAQASAPMPFGPYLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=245662 CLM75_RS04060 WP_098465764.1 893685..894551(-) (pilD) [Pseudomonas lurida strain MYb11]
TTGAGCCTGCTGCTGGCTGGCCACCCCTGGGCATTTATTGCGCTGGCGTCAGTGCTGGGACTGATCGTCGGCAGTTTTCT
CAACGTGCTGGTGTGGCGCCTGCCAAAGATGCTCGAACGGGAATGGCGTGCGCAGGCCCACGAAGTACTCGGCTTGCCCG
CTGAACCTGCCGGCCCGACCTACAACCTGATGCACCCCAACTCCTGCTGCCCGCAGTGCAACCATCCGATACGCCCGAGG
GAAAACATCCCACTGCTCAGCTACCTGTTTCTGCGAGGCCGCTGCGCCCAATGCCAAAAGGCAATCAGCGTCCGTTATCC
GCTCACTGAACTGGCGTGCGGGTTGATCTCGGCCTGGGTTGCCTGGCATTTCGGCTTTGGCTGGCCAGCGGGCGCGGTGC
TGGTACTGAGTTGGGGGTTGTTGGCGATGAGCCTGATCGATGCCGATCACCAACTGTTGCCGGATGTGCTGGTGATGCCG
CTGCTGTGGCTGGGGCTGATCGTCAACGGGTTCGGACTGCTGACGACACTGCCCGATGCCCTGTGGGGCGCGGTGATCGG
GTACATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTTACCGGCAAGGACGGCATGGGCCATGGTGACTTCAAGC
TGCTGGCGCTGCTCGGTGCCTGGGGCGGCTGGCAGATTCTGCCGATGACGCTGTTGATGTCGTCACTGGTAGGTGTGTTC
GTGGGCTTGATCCTGATGCGCCTGCGCAAGGCCCAGGCATCGGCACCGATGCCATTTGGTCCGTATCTGGCGATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.763	96.875	0.521
pilD	Vibrio campbellii strain DS40M4	51.071	97.222	0.497
pilD	Neisseria gonorrhoeae MS11	50.741	93.75	0.476
pilD	Acinetobacter baumannii D1279779	48.718	94.792	0.462
pilD	Acinetobacter nosocomialis M2	48.718	94.792	0.462