Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CK627_RS01585 Genome accession   NZ_CP023141
Coordinates   286568..288274 (+) Length   568 a.a.
NCBI ID   WP_024943110.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain KN-Mc-6U21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 281568..293274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK627_RS01555 (CK627_01555) pdhR 282743..283507 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  CK627_RS01560 (CK627_01560) ampD 283783..284355 (-) 573 WP_095591544.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CK627_RS01565 (CK627_01565) - 284479..284949 (+) 471 WP_095591545.1 TIGR02281 family clan AA aspartic protease -
  CK627_RS01570 (CK627_01570) nadC 284954..285811 (+) 858 WP_095591546.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CK627_RS01580 (CK627_01580) tapA 286133..286561 (+) 429 WP_041204773.1 type IVa pilus major pilin TapA -
  CK627_RS01585 (CK627_01585) pilB 286568..288274 (+) 1707 WP_024943110.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CK627_RS01590 (CK627_01590) pilC 288390..289631 (+) 1242 WP_005305649.1 type II secretion system F family protein Machinery gene
  CK627_RS01595 (CK627_01595) pilD 289654..290526 (+) 873 WP_095591547.1 A24 family peptidase Machinery gene
  CK627_RS01600 (CK627_01600) coaE 290546..291160 (+) 615 WP_005305656.1 dephospho-CoA kinase -
  CK627_RS01605 (CK627_01605) zapD 291198..291920 (+) 723 WP_026141277.1 cell division protein ZapD -
  CK627_RS01610 (CK627_01610) yacG 291930..292124 (+) 195 WP_017782442.1 DNA gyrase inhibitor YacG -
  CK627_RS01615 (CK627_01615) mutT 292185..292598 (-) 414 WP_026141640.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62538.94 Da        Isoelectric Point: 5.5619

>NTDB_id=244919 CK627_RS01585 WP_024943110.1 286568..288274(+) (pilB) [Aeromonas dhakensis strain KN-Mc-6U21]
MTSSPHSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=244919 CK627_RS01585 WP_024943110.1 286568..288274(+) (pilB) [Aeromonas dhakensis strain KN-Mc-6U21]
ATGACCTCTAGCCCCCATAGCGGCCTGGCGTTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGACCAAGTACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGACGGCTGCCCCAGGATGGCCGCATCAAATTGAAGATGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACTCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAAATCAACCTGCCTGGGGTCAATCAGGTGCAGGTCAACCCCAAGG
CTGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTGATGGTCGGGGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCCCAGC
GACTGGCACGCAAGCTGTGCGACCACTGCAAGGCCCCCGAGGTGGTGCCGGAGGCTGAACTGCTGGAGTTGGGGTTCACC
CAACAGCAACTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGCTGCAAGGAGTGTTCTGGCGGCTACAAGGGACGGGT
CGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAATTCCATGCAGATTGCGG
CCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGCACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.282

100

0.553
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment