Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSYR_RS02115 Genome accession   NC_007005
Coordinates   436455..438560 (+) Length   701 a.a.
NCBI ID   WP_164920435.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae B728a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431455..443560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYR_RS02095 (Psyr_0403) comM 433624..434688 (+) 1065 WP_003403040.1 pilus assembly protein PilM Machinery gene
  PSYR_RS02100 (Psyr_0404) - 434688..435263 (+) 576 WP_003403038.1 PilN domain-containing protein -
  PSYR_RS02105 (Psyr_0405) pilO 435260..435883 (+) 624 WP_003403037.1 type 4a pilus biogenesis protein PilO -
  PSYR_RS02110 (Psyr_0406) pilP 435880..436407 (+) 528 WP_011266376.1 type 4a pilus biogenesis lipoprotein PilP -
  PSYR_RS02115 (Psyr_0407) pilQ 436455..438560 (+) 2106 WP_164920435.1 type IV pilus secretin PilQ Machinery gene
  PSYR_RS02120 (Psyr_0408) aroK 438565..439083 (+) 519 WP_011266378.1 shikimate kinase AroK -
  PSYR_RS02125 (Psyr_0409) aroB 439198..440301 (+) 1104 WP_003403032.1 3-dehydroquinate synthase -
  PSYR_RS02130 (Psyr_0410) - 440312..441934 (+) 1623 WP_011266379.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75370.80 Da        Isoelectric Point: 5.0976

>NTDB_id=24318 PSYR_RS02115 WP_164920435.1 436455..438560(+) (pilQ) [Pseudomonas syringae pv. syringae B728a]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=24318 PSYR_RS02115 WP_164920435.1 436455..438560(+) (pilQ) [Pseudomonas syringae pv. syringae B728a]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCTGCACCGCGCG
GTTACACCACCTCGCAACCGGCGCGCATTGCGCTGGACCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGCAATGCGCGCAGTGTGGTCGTGGTACAGGCTCAGGACCGTACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACTACCGCGATGGGCGCGCAACGGGTCGCGGTGCCACCGCCCACACCGGCATCCGCCAAGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGACTTTCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATTGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGACAGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGAC
CAACGAGGATCTGGACCGCCGCGCTGCCGACAAGCCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
TGGCAACGTTCTGCTGGTCGCACCAGCCGATGAAATTGCCGCCCGTGAGCGTCAGGAGCTGGAATCGCTCAAACAGATCG
CCGAGCTGGCGCCCCTGCGCCGCGAACTGCTGCAGGTCAATTACGCCAAGGCAGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGCGCCGAGTCCAAGGCCGACGAGCGCGGCTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAACTGCGTCGTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTACGCTGGGGCGGGTCTACCAACACCAGCGGCAGCGGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTT
CGTGGACCTCGGCGCAGCCGGCGCTACCTCGGGTATCGGCCTTGGCTTCGTGACCAACAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAAGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGCGTACCGCCCATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.917

100

0.749
  comQ Acinetobacter baylyi ADP1

42.203

99.715

0.421
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.897

98.288

0.382
  pilQ Legionella pneumophila strain Lp02

38.865

98.003

0.381

Multiple sequence alignment