Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CCZ37_RS02415 Genome accession   NZ_CP022741
Coordinates   475623..477311 (-) Length   562 a.a.
NCBI ID   WP_094499654.1    Uniprot ID   A0A223MVA2
Organism   Vibrio qinghaiensis strain Q67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470623..482311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCZ37_RS02385 (CCZ37_02380) rplS 471341..471694 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  CCZ37_RS02390 (CCZ37_02385) yacG 471775..471972 (-) 198 WP_094499651.1 DNA gyrase inhibitor YacG -
  CCZ37_RS02395 (CCZ37_02390) zapD 472062..472802 (-) 741 WP_010319527.1 cell division protein ZapD -
  CCZ37_RS02400 (CCZ37_02395) coaE 472835..473443 (-) 609 WP_094499652.1 dephospho-CoA kinase -
  CCZ37_RS02405 (CCZ37_02400) pilD 473434..474315 (-) 882 WP_094499653.1 A24 family peptidase Machinery gene
  CCZ37_RS02410 (CCZ37_02405) pilC 474354..475580 (-) 1227 WP_047688859.1 type II secretion system F family protein Machinery gene
  CCZ37_RS02415 (CCZ37_02410) pilB 475623..477311 (-) 1689 WP_094499654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCZ37_RS19560 (CCZ37_02415) - 477320..477739 (-) 420 WP_279627423.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CCZ37_RS02425 (CCZ37_02420) nadC 477999..478886 (-) 888 WP_010319533.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CCZ37_RS02430 (CCZ37_02425) ampD 479011..479562 (+) 552 WP_234826552.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CCZ37_RS02435 (CCZ37_02430) fldB 479584..480102 (-) 519 WP_010319535.1 flavodoxin FldB -
  CCZ37_RS02440 (CCZ37_02435) xerD 480255..481163 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  CCZ37_RS02445 (CCZ37_02440) - 481177..481950 (+) 774 WP_010319537.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62177.27 Da        Isoelectric Point: 6.1489

>NTDB_id=242056 CCZ37_RS02415 WP_094499654.1 475623..477311(-) (pilB) [Vibrio qinghaiensis strain Q67]
MLTNLPAILRQANLISLAQEQAVIEHIKASGASVPDALLALDFFHPDDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIAHNGQTLTIAVSDPTHLQVEDDFRFATGLQVEVVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=242056 CCZ37_RS02415 WP_094499654.1 475623..477311(-) (pilB) [Vibrio qinghaiensis strain Q67]
ATGCTAACCAACCTACCCGCAATTCTTCGGCAAGCAAATCTTATTAGCCTTGCGCAAGAGCAAGCTGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGACGCTTTGCTCGCTTTGGATTTTTTCCATCCCGATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCCATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTTGCGACAGGCTTACAAGTCGAAGTTGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTCTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCAGACATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCCGAAGATCCGGTTGAAATTAACTTATCCGGCATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTAATGGTTGGCGAAATCCGCGATTTAGAAACC
GCCGAAATCGCCGTAAAAGCAGCACAAACCGGGCATTTAGTCCTTTCTACCTTGCATACTAACTCCGCCGCTGAAACGGT
GATTCGCTTAAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTCACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223MVA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.274

100

0.833
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Vibrio parahaemolyticus RIMD 2210633

72.776

100

0.728
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.438
  pilF Thermus thermophilus HB27

38.393

99.644

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

35.69

100

0.377

Multiple sequence alignment