Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Kalk_RS15680 Genome accession   NZ_CP022684
Coordinates   3681069..3683147 (+) Length   692 a.a.
NCBI ID   WP_101895146.1    Uniprot ID   A0A2K9LNQ5
Organism   Ketobacter alkanivorans strain GI5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3676069..3688147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS15660 (Kalk_15635) comM 3678248..3679312 (+) 1065 WP_101895142.1 pilus assembly protein PilM Machinery gene
  Kalk_RS15665 (Kalk_15640) - 3679312..3679869 (+) 558 WP_101895143.1 PilN domain-containing protein -
  Kalk_RS15670 (Kalk_15645) - 3679872..3680465 (+) 594 WP_101895144.1 type 4a pilus biogenesis protein PilO -
  Kalk_RS15675 (Kalk_15650) - 3680467..3680997 (+) 531 WP_101895145.1 pilus assembly protein PilP -
  Kalk_RS15680 (Kalk_15655) pilQ 3681069..3683147 (+) 2079 WP_101895146.1 type IV pilus secretin PilQ Machinery gene
  Kalk_RS15685 (Kalk_15660) aroK 3683151..3683672 (+) 522 WP_101895147.1 shikimate kinase AroK -
  Kalk_RS15690 (Kalk_15665) aroB 3683785..3684870 (+) 1086 WP_101895148.1 3-dehydroquinate synthase -
  Kalk_RS15695 (Kalk_15670) - 3684848..3686419 (+) 1572 WP_101895149.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74474.34 Da        Isoelectric Point: 4.3862

>NTDB_id=241654 Kalk_RS15680 WP_101895146.1 3681069..3683147(+) (pilQ) [Ketobacter alkanivorans strain GI5]
MKFKFCWGAVFALLSTPLFAAQLTGVESAALPGDGIEIKLKFDSAPPEPSGYTTDSPARIAIDLDGVSNGLTEKYITLGN
GNVRSLTVMGAGTRTRLIVNMSSLVGYTTTVSGNNLLLRVGDAAPSTTAGAKPVSGVERVASAPAKARADGVQEIDFRRG
EQGEGQVVITLSNPNAPVDLIEEGGKIVAEFVGVTLPDQLHRRLDVMDFATPVKLIDATTASGNARIAVTPTGEYEYMAY
QTDQTFTISVKPISTVELERKRKEKFQYTGEKLSLNFQDIEVRSVLQLIADFTELNLVASDTVRGRITLRLQNVPWDQAL
DLILKTKGLDKRKVGNVMLVAPADEIAAREKLELESQRQVKELAPLRTEYLTVNYAKAGEVAELVTNSGFLSERGTITVD
ERTNVLLIQDTSGNIDEVRFMLEQLDIPVRQVMIEARIVIANTDAVEELGVRWGLGAFNIGSDRLSYLGGNANGAEGNIA
NIVNGDDFEIGTGTLALDLGTVSDLAATSASRITFGFFDADNGLIDLELSALESDGRADIVSQPKVMTADQQTAKIQSGT
EVPYQEASSSGATSTSFKEAVLSLEVTPQITPDGRVIMDLKVNQDSVGDIFNGVPSIETNEIETSVIVDNGQTVVLGGVF
RSQEVHEVVKTPFLGDLPVIGRLFRRTLESSEKAELLVFITPRIVNDVLAAQ

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=241654 Kalk_RS15680 WP_101895146.1 3681069..3683147(+) (pilQ) [Ketobacter alkanivorans strain GI5]
ATGAAATTTAAGTTCTGTTGGGGCGCTGTGTTTGCCCTGCTGTCCACTCCGCTTTTTGCCGCACAATTGACCGGCGTAGA
GTCTGCCGCTCTACCTGGGGATGGCATCGAAATCAAACTGAAATTTGACAGTGCGCCACCTGAGCCTTCGGGTTACACCA
CTGATTCACCGGCGCGGATTGCAATAGATCTGGATGGTGTTAGCAATGGGTTAACTGAAAAGTACATAACCTTGGGCAAC
GGTAATGTGCGCAGTCTGACTGTAATGGGGGCCGGCACTCGCACCCGCCTTATTGTTAACATGAGTTCACTGGTTGGGTA
TACAACGACGGTGAGTGGTAACAACCTGTTGTTACGCGTTGGTGATGCCGCGCCGTCGACTACTGCGGGTGCCAAGCCGG
TGTCCGGTGTTGAACGGGTAGCGTCAGCGCCCGCGAAGGCTAGGGCTGACGGTGTTCAAGAAATCGATTTTCGTCGTGGC
GAGCAGGGTGAAGGGCAAGTAGTAATTACATTGTCTAATCCTAATGCTCCAGTGGACTTGATTGAGGAAGGTGGCAAGAT
TGTGGCTGAGTTTGTTGGTGTCACCTTGCCAGACCAGCTTCATCGCAGACTGGATGTGATGGACTTTGCTACACCTGTCA
AATTGATCGATGCCACCACGGCATCTGGAAACGCCCGCATTGCGGTGACACCTACCGGTGAATACGAGTACATGGCCTAC
CAAACTGATCAGACTTTCACCATTAGCGTGAAGCCTATCAGTACCGTAGAGCTTGAGCGCAAGCGCAAAGAGAAATTCCA
GTACACCGGTGAGAAGCTGTCCTTGAACTTTCAGGATATCGAGGTGCGCTCGGTACTGCAGTTGATTGCCGATTTCACCG
AGCTTAACTTGGTTGCCAGTGACACTGTGCGCGGCCGGATCACCTTGCGATTGCAGAATGTTCCCTGGGATCAGGCCCTG
GATCTGATTTTGAAGACCAAAGGTCTCGATAAGCGTAAAGTCGGCAACGTTATGCTGGTTGCTCCTGCTGATGAAATAGC
GGCGCGAGAAAAGCTCGAGCTGGAAAGTCAGCGTCAGGTTAAAGAACTGGCGCCTTTGCGTACAGAATACCTGACTGTTA
ATTATGCCAAAGCGGGTGAAGTGGCTGAGCTGGTGACCAATTCAGGCTTCCTCAGTGAGCGTGGAACCATAACGGTGGAT
GAGCGTACCAACGTTCTGCTGATCCAGGATACCTCCGGCAATATCGATGAAGTTCGCTTTATGTTGGAGCAGTTGGACAT
TCCTGTACGACAGGTGATGATCGAGGCTCGTATCGTGATTGCCAATACAGATGCGGTAGAGGAGCTTGGTGTTCGCTGGG
GCTTGGGCGCATTCAATATCGGCAGTGATCGTCTGTCATATTTGGGTGGTAACGCGAACGGAGCCGAAGGCAATATTGCC
AATATCGTGAATGGCGATGATTTTGAGATCGGCACTGGTACGCTGGCTTTGGATTTAGGTACGGTTTCTGATTTGGCAGC
AACATCTGCCAGCCGTATTACCTTCGGCTTCTTTGATGCAGATAACGGGCTTATTGATCTTGAGCTGTCCGCCCTTGAGT
CGGATGGTCGGGCTGACATCGTTTCCCAGCCGAAGGTCATGACTGCGGATCAGCAAACAGCCAAGATTCAGTCTGGTACA
GAGGTTCCTTATCAGGAAGCTTCCTCCAGCGGTGCAACCTCAACTTCATTTAAAGAGGCGGTGCTGTCTCTGGAAGTGAC
GCCACAGATCACACCGGACGGACGGGTCATTATGGATCTGAAAGTTAACCAGGACTCTGTAGGTGACATATTCAATGGTG
TGCCTTCTATCGAAACCAATGAGATCGAAACCAGTGTCATCGTAGACAATGGTCAGACGGTAGTGCTGGGTGGTGTGTTT
AGATCTCAGGAAGTCCATGAAGTGGTTAAAACCCCTTTCCTGGGTGATCTCCCTGTGATCGGCCGCCTGTTCCGTCGAAC
GCTGGAGTCGTCAGAGAAAGCAGAGCTGCTGGTGTTCATCACTCCACGTATTGTGAATGATGTGTTGGCTGCCCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-685)

AMIN domain (AMIN)

(34-119)

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LNQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

53.125

100

0.54
  comQ Acinetobacter baylyi ADP1

44.598

100

0.465
  pilQ Acinetobacter baumannii strain A118

42.779

100

0.454
  pilQ Acinetobacter baumannii D1279779

42.779

100

0.454
  pilQ Legionella pneumophila strain ERS1305867

40.175

99.277

0.399
  pilQ Legionella pneumophila strain Lp02

40.029

99.277

0.397
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.424

85.838

0.364
  pilQ Vibrio cholerae strain A1552

42.424

85.838

0.364

Multiple sequence alignment