Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   Kalk_RS15660 Genome accession   NZ_CP022684
Coordinates   3678248..3679312 (+) Length   354 a.a.
NCBI ID   WP_101895142.1    Uniprot ID   A0A2K9LNF1
Organism   Ketobacter alkanivorans strain GI5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3673248..3684312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS15655 (Kalk_15630) - 3675539..3678037 (-) 2499 WP_101895141.1 penicillin-binding protein 1A -
  Kalk_RS15660 (Kalk_15635) comM 3678248..3679312 (+) 1065 WP_101895142.1 pilus assembly protein PilM Machinery gene
  Kalk_RS15665 (Kalk_15640) - 3679312..3679869 (+) 558 WP_101895143.1 PilN domain-containing protein -
  Kalk_RS15670 (Kalk_15645) - 3679872..3680465 (+) 594 WP_101895144.1 type 4a pilus biogenesis protein PilO -
  Kalk_RS15675 (Kalk_15650) - 3680467..3680997 (+) 531 WP_101895145.1 pilus assembly protein PilP -
  Kalk_RS15680 (Kalk_15655) pilQ 3681069..3683147 (+) 2079 WP_101895146.1 type IV pilus secretin PilQ Machinery gene
  Kalk_RS15685 (Kalk_15660) aroK 3683151..3683672 (+) 522 WP_101895147.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37967.05 Da        Isoelectric Point: 4.2236

>NTDB_id=241653 Kalk_RS15660 WP_101895142.1 3678248..3679312(+) (comM) [Ketobacter alkanivorans strain GI5]
MLPFLSKKASTAKLGIDISSTSVKLLELSRSGNRYKVEAYAVEPLPANAVVEKNINDVEGVGEAIARVVARAKSGVKNAA
VAVAGSSVITKVIEMDGKLSEDEMESQIKVEADQYIPYPLDEVAIDFEVQGTVEGSADQVEVLLAACRNENVELRVDVLE
IAGLTAKVMDVEAYAMERAFGLVADQIDGGEEQTVAIVDIGSTMTTLSVLHDGRTIYTREQLFGGKQLTEEIQRRYGLSF
EEAGLAKKQGGLPDDYETEVLEPFKEAVVQQVTRSLQFFFSSSQYNDVDHILLAGGTASIPGLAEMVQDKVGTTTSIANP
FVDMALSSKVNASLLSADAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=241653 Kalk_RS15660 WP_101895142.1 3678248..3679312(+) (comM) [Ketobacter alkanivorans strain GI5]
ATGCTGCCCTTCCTCAGTAAGAAGGCTTCAACAGCCAAGTTGGGAATCGATATCAGCTCGACATCGGTCAAATTGCTCGA
GCTCAGTCGCAGTGGTAATCGCTACAAGGTCGAGGCCTATGCGGTAGAGCCATTGCCAGCCAACGCCGTAGTCGAAAAGA
ATATTAATGATGTGGAGGGTGTTGGCGAAGCCATCGCCCGCGTCGTGGCTCGAGCCAAGAGTGGCGTTAAAAACGCTGCG
GTGGCTGTTGCTGGCTCTTCGGTGATCACCAAGGTCATCGAAATGGACGGCAAACTCTCCGAAGACGAGATGGAGAGCCA
GATCAAGGTTGAGGCCGACCAGTACATTCCCTATCCACTGGATGAAGTTGCCATCGATTTCGAGGTGCAAGGCACGGTCG
AAGGCTCTGCTGATCAGGTGGAAGTGCTGCTGGCAGCCTGCCGCAACGAAAACGTCGAGTTGCGGGTAGATGTCCTGGAG
ATCGCCGGACTTACCGCCAAGGTGATGGATGTTGAGGCCTACGCCATGGAGCGTGCCTTCGGTTTGGTAGCGGATCAGAT
CGATGGCGGTGAAGAGCAGACGGTAGCGATTGTTGATATCGGCTCTACCATGACCACCCTCAGCGTTCTGCATGATGGCC
GCACTATCTACACTCGAGAGCAGTTGTTCGGCGGTAAGCAGCTGACCGAAGAAATTCAACGTCGTTACGGGTTGTCCTTC
GAAGAAGCGGGTCTGGCCAAGAAACAAGGCGGTCTACCCGATGACTACGAGACGGAAGTCTTGGAACCCTTCAAGGAAGC
CGTTGTACAACAAGTAACCAGATCCTTGCAGTTTTTCTTCTCCTCCAGCCAATATAACGATGTGGATCACATCCTGCTGG
CAGGGGGCACAGCCTCAATTCCCGGCCTGGCCGAGATGGTGCAGGATAAAGTAGGCACCACCACTTCCATAGCCAACCCG
TTTGTGGACATGGCTCTTTCCTCGAAGGTAAACGCGTCTCTACTCAGCGCCGATGCGCCATCACTGATGATCGCCTGCGG
CTTGGCGTTAAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LNF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

59.714

98.87

0.59
  pilM Acinetobacter baumannii D1279779

59.429

98.87

0.588
  comM Acinetobacter baylyi ADP1

56.857

98.87

0.562
  pilM Legionella pneumophila strain ERS1305867

48.451

100

0.486

Multiple sequence alignment