Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CHR29_RS26085 Genome accession   NZ_CP022562
Coordinates   5559189..5560457 (-) Length   422 a.a.
NCBI ID   WP_016712494.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5554189..5565457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR29_RS26070 (CHR29_25945) - 5555842..5557455 (-) 1614 WP_016712491.1 SPOR domain-containing protein -
  CHR29_RS26075 (CHR29_25950) aroB 5557471..5558568 (-) 1098 WP_028698446.1 3-dehydroquinate synthase -
  CHR29_RS26080 (CHR29_25955) aroK 5558666..5559184 (-) 519 WP_028698445.1 shikimate kinase AroK -
  CHR29_RS26085 (CHR29_25960) pilQ 5559189..5560457 (-) 1269 WP_016712494.1 type IV pilus secretin PilQ Machinery gene
  CHR29_RS26090 (CHR29_25965) - 5560381..5561382 (-) 1002 WP_028698443.1 pilus assembly protein PilP -
  CHR29_RS26095 (CHR29_25970) - 5561394..5561930 (-) 537 WP_016712496.1 PilN domain-containing protein -
  CHR29_RS26100 (CHR29_25975) pilM 5561927..5562808 (-) 882 WP_016712497.1 type IV pilus biogenesis protein PilM -
  CHR29_RS26105 (CHR29_25980) - 5562999..5565452 (+) 2454 WP_016712498.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45995.75 Da        Isoelectric Point: 4.7088

>NTDB_id=240830 CHR29_RS26085 WP_016712494.1 5559189..5560457(-) (pilQ) [Pseudomonas monteilii strain B5]
MKMLMKWVLAALLPIPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVAGDDVQGNVTLRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAAFAAQPFDAPLGLEPNAQVQPLRRELIPIHHAKAAELVDLLLTSLADDGTLTARGSLTVDE
RTNTLVAYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYSENLRPGQELFVDLGVERAGSSL
GLGLLRGDLLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=240830 CHR29_RS26085 WP_016712494.1 5559189..5560457(-) (pilQ) [Pseudomonas monteilii strain B5]
ATGAAAATGTTGATGAAATGGGTGTTGGCAGCCCTGCTGCCGATCCCGCTGGCGTCGGCGATGCCTTACCAGGGTGAGCC
CCTGTCGTTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGATTATGCGGGCGTCAACCTGGTTG
CGGGTGACGACGTACAAGGTAACGTCACGTTGCGTTTACAGGATGTACCTTGGGATCAGGCACTCGACCTGGTGTTGCGA
AGCAAGGGCCTGGCCCGGCGGCAGGAGGGTAATGTACTGCTGGTGGCACCTGCGGCAGCGTTTGCCGCGCAGCCGTTCGA
TGCCCCCTTGGGGCTGGAGCCGAATGCTCAGGTGCAGCCGCTACGCCGTGAGCTGATACCAATCCATCATGCCAAGGCCG
CCGAGCTGGTTGATCTATTGCTGACCAGCCTGGCGGATGACGGCACCCTCACTGCCAGGGGCAGCCTGACTGTCGACGAG
CGCACCAATACACTGGTGGCGTACCAGCCCGCCGACCGCCTGGCGGAACTGCGGCAGCTGGTTGCGCAACTGGACGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTCGAAGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGCG
GGCCGTTGTATAGCGAAAACCTGCGGCCGGGCCAGGAGCTGTTTGTCGATCTTGGTGTGGAGCGTGCGGGCAGTAGTCTC
GGCCTGGGCCTGCTGCGTGGCGACCTGTTGCTCGACCTGGAGCTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCAT
TTCGCAGCCCAAAGTGGTAACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCCTACCAGGAAACCA
GTCAAAGTGGCGCCACCTCGGTGTCGTTTCGTGAAGCCTCGCTGTCGCTGGAGGTGACACCGCAAATCACCCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCGGACGGTGAAACCATCGTGATTGGTGGCGTGTACTCCACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(50-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  comQ Acinetobacter baylyi ADP1

42.444

100

0.453
  pilQ Acinetobacter baumannii strain A118

41.266

100

0.448
  pilQ Acinetobacter baumannii D1279779

41.266

100

0.448
  pilQ Vibrio campbellii strain DS40M4

40.323

100

0.415
  pilQ Legionella pneumophila strain Lp02

41.627

99.052

0.412
  pilQ Legionella pneumophila strain ERS1305867

41.627

99.052

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.093

99.763

0.41
  pilQ Vibrio cholerae strain A1552

41.093

99.763

0.41

Multiple sequence alignment