Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CHR26_RS09490 Genome accession   NZ_CP022560
Coordinates   2008108..2009370 (+) Length   420 a.a.
NCBI ID   WP_145913582.1    Uniprot ID   -
Organism   Pseudomonas putida strain B1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2003108..2014370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR26_RS09475 (CHR26_09405) pilM 2005757..2006638 (+) 882 WP_145913580.1 type IV pilus biogenesis protein PilM -
  CHR26_RS09480 (CHR26_09410) - 2006635..2007171 (+) 537 WP_016489625.1 PilN domain-containing protein -
  CHR26_RS09485 (CHR26_09415) - 2007168..2008184 (+) 1017 WP_145913581.1 pilus assembly protein PilP -
  CHR26_RS09490 (CHR26_09420) pilQ 2008108..2009370 (+) 1263 WP_145913582.1 type IV pilus secretin PilQ Machinery gene
  CHR26_RS09495 (CHR26_09425) aroK 2009375..2009893 (+) 519 WP_024717571.1 shikimate kinase AroK -
  CHR26_RS09500 (CHR26_09430) aroB 2009990..2011087 (+) 1098 WP_024717572.1 3-dehydroquinate synthase -
  CHR26_RS09505 (CHR26_09435) - 2011103..2012707 (+) 1605 WP_145913583.1 SPOR domain-containing protein -
  CHR26_RS09510 - 2012837..2013061 (+) 225 WP_132844974.1 hypothetical protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45911.62 Da        Isoelectric Point: 4.9870

>NTDB_id=240778 CHR26_RS09490 WP_145913582.1 2008108..2009370(+) (pilQ) [Pseudomonas putida strain B1]
MKHVMKWVLAALLPIPLVWATPYQGEPLSLNFQDVEVRSVLQVLADYAGINLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLAQRHEGNVLLVAPAAAFAPQPFDAHPVPDAQMQPLRRELIPIHHAKAAELVELLLTSLADDSTFTVRGSLGVDERT
NTLVAHQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGESLRPGKELFVDLGVERAGSSVGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVAFREASLSLEVTPQITPDNKV
IMAVRVTKDEPDYVNAVNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDVLQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=240778 CHR26_RS09490 WP_145913582.1 2008108..2009370(+) (pilQ) [Pseudomonas putida strain B1]
ATGAAGCACGTGATGAAATGGGTGTTGGCGGCCCTGCTGCCGATCCCGCTGGTGTGGGCGACGCCGTATCAGGGTGAGCC
ACTTTCTTTGAATTTCCAGGATGTAGAGGTTCGCTCGGTGCTGCAGGTGCTGGCCGATTATGCGGGCATCAATCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGCTTGCAGGACGTGCCCTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGGCACAGCGGCACGAAGGCAATGTGTTGCTGGTGGCCCCTGCGGCAGCGTTTGCCCCGCAGCCGTTCGA
TGCCCATCCGGTGCCGGATGCGCAGATGCAGCCGTTACGCCGTGAACTGATACCGATCCATCATGCCAAGGCGGCGGAGC
TGGTTGAGTTGTTGCTGACCAGCCTGGCGGATGACAGCACCTTCACCGTTCGTGGCAGCTTGGGTGTCGATGAGCGCACC
AATACCCTGGTGGCGCATCAGCCGGCCGATCGCCTGGCCGAATTGCGGCAACTGGTTGCGCAGCTGGACGTGCCGGTGCG
CCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGACTACGAGAAAAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TGTACGGGGAAAGCCTGCGCCCGGGCAAGGAACTGTTCGTCGACCTCGGCGTGGAGCGGGCGGGCAGCAGCGTCGGCCTG
GGCCTGCTGCGCGGTGACGTGTTGCTCGACCTGGAACTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCATCTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCAGCCAGA
GTGGCGCCACCTCGGTCGCCTTCCGCGAAGCCTCGCTTTCGCTGGAGGTGACCCCGCAGATTACACCTGACAACAAGGTA
ATCATGGCTGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCCGTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(123-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.998

100

0.617
  pilQ Acinetobacter baumannii strain A118

42.241

100

0.467
  pilQ Acinetobacter baumannii D1279779

42.241

100

0.467
  comQ Acinetobacter baylyi ADP1

43.111

100

0.462
  pilQ Legionella pneumophila strain Lp02

42.143

100

0.421
  pilQ Legionella pneumophila strain ERS1305867

42.143

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.941

100

0.414
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.487

99.286

0.412
  pilQ Vibrio cholerae strain A1552

41.487

99.286

0.412

Multiple sequence alignment