Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFH90_RS15885 Genome accession   NZ_CP022298
Coordinates   3222687..3224399 (+) Length   570 a.a.
NCBI ID   WP_126038504.1    Uniprot ID   A0A3Q8XFW8
Organism   Acinetobacter johnsonii strain IC001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3217687..3229399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFH90_RS15840 (CFH90_15780) nusA 3218273..3219757 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  CFH90_RS15845 (CFH90_15785) rimP 3219797..3220321 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  CFH90_RS15875 (CFH90_15815) secG 3221273..3221602 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  CFH90_RS15880 (CFH90_15820) tpiA 3221616..3222407 (-) 792 WP_126038501.1 triose-phosphate isomerase -
  CFH90_RS15885 (CFH90_15825) pilB 3222687..3224399 (+) 1713 WP_126038504.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFH90_RS15890 (CFH90_15830) pilC 3224430..3225656 (+) 1227 WP_004692713.1 type II secretion system F family protein Machinery gene
  CFH90_RS15895 (CFH90_15835) pilD 3225656..3226516 (+) 861 WP_126038508.1 prepilin peptidase Machinery gene
  CFH90_RS15900 (CFH90_15840) coaE 3226523..3227122 (+) 600 WP_126038510.1 dephospho-CoA kinase -
  CFH90_RS15905 (CFH90_15845) - 3227136..3228053 (-) 918 WP_164836593.1 DMT family transporter -
  CFH90_RS15910 (CFH90_15850) rlmB 3228086..3228835 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CFH90_RS15915 (CFH90_15855) - 3228979..3229302 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63630.04 Da        Isoelectric Point: 4.9462

>NTDB_id=238209 CFH90_RS15885 WP_126038504.1 3222687..3224399(+) (pilB) [Acinetobacter johnsonii strain IC001]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEYPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKVPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=238209 CFH90_RS15885 WP_126038504.1 3222687..3224399(+) (pilB) [Acinetobacter johnsonii strain IC001]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGAGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAATATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCGCAAACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGTAATATGGGGGTTCCCTCTTTTAACATTGCGACATCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGTTCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGTTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q8XFW8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.811
  pilB Acinetobacter baylyi ADP1

76.491

100

0.765
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.501

99.474

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

90.702

0.463
  pilB Vibrio campbellii strain DS40M4

50

90.877

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.304

100

0.372
  pilF Thermus thermophilus HB27

36.888

100

0.37

Multiple sequence alignment