Detailed information NTG0238211

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CFH90_RS15895
• Genome accession: NZ_CP022298
• Coordinates: 3225656..3226516 (+)
• Length: 286 a.a.
• NCBI ID: WP_126038508.1
• Uniprot ID: A0A3S9APA7
• Organism: Acinetobacter johnsonii strain IC001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3220656..3231516

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFH90_RS15875 (CFH90_15815)	secG	3221273..3221602 (-)	330	WP_004692710.1	preprotein translocase subunit SecG	-
CFH90_RS15880 (CFH90_15820)	tpiA	3221616..3222407 (-)	792	WP_126038501.1	triose-phosphate isomerase	-
CFH90_RS15885 (CFH90_15825)	pilB	3222687..3224399 (+)	1713	WP_126038504.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CFH90_RS15890 (CFH90_15830)	pilC	3224430..3225656 (+)	1227	WP_004692713.1	type II secretion system F family protein	Machinery gene
CFH90_RS15895 (CFH90_15835)	pilD	3225656..3226516 (+)	861	WP_126038508.1	prepilin peptidase	Machinery gene
CFH90_RS15900 (CFH90_15840)	coaE	3226523..3227122 (+)	600	WP_126038510.1	dephospho-CoA kinase	-
CFH90_RS15905 (CFH90_15845)	-	3227136..3228053 (-)	918	WP_164836593.1	DMT family transporter	-
CFH90_RS15910 (CFH90_15850)	rlmB	3228086..3228835 (-)	750	WP_004692717.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
CFH90_RS15915 (CFH90_15855)	-	3228979..3229302 (-)	324	WP_004692718.1	pyrimidine/purine nucleoside phosphorylase	-
CFH90_RS15920 (CFH90_15860)	recN	3229699..3231360 (+)	1662	WP_126038513.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31956.35 Da
• Isoelectric Point: 8.0620

>NTDB_id=238211 CFH90_RS15895 WP_126038508.1 3225656..3226516(+) (pilD) [Acinetobacter johnsonii strain IC001]
MQEIISYLINTPMALYFAVGVFSLCIGSFLNVVIYRTPKMMEQEWRTDCQLLLHPEQPIIDETKLSLSKPASTCPKCKSA
IHWYQNVPVMSWFLLRGKCGTCHNPISIRYPAIELLTAICALVVVAVFGPTIQMLFGLVLTYILIALTFIDFDTQLLPDR
YTLTLAALGLGVNSYAIYTTPTAAIWGYIIGFLCLWIVYYLFKLITGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSF
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKIYLGN

Nucleotide

• Length: 861 bp

>NTDB_id=238211 CFH90_RS15895 WP_126038508.1 3225656..3226516(+) (pilD) [Acinetobacter johnsonii strain IC001]
ATGCAAGAAATAATTTCCTATCTGATCAATACTCCAATGGCGCTGTATTTCGCCGTTGGAGTTTTTAGTTTGTGCATCGG
TAGTTTCCTAAATGTGGTGATCTATCGCACGCCAAAAATGATGGAACAAGAATGGCGCACCGACTGCCAACTTCTGCTTC
ATCCTGAGCAACCGATTATTGATGAAACGAAACTCTCGTTGAGTAAGCCTGCCTCGACCTGCCCCAAATGCAAAAGTGCC
ATACACTGGTATCAAAATGTCCCTGTGATGAGTTGGTTCCTGCTACGAGGAAAGTGTGGCACTTGTCATAACCCGATCAG
CATTCGCTACCCTGCGATTGAGCTCCTGACCGCCATTTGTGCATTGGTCGTGGTTGCAGTCTTTGGCCCAACAATCCAAA
TGCTATTCGGTCTCGTACTCACTTACATTTTAATCGCCTTAACCTTTATTGATTTTGACACTCAACTTTTACCTGATCGC
TATACCCTCACCCTTGCAGCTCTGGGTTTAGGGGTCAATAGTTATGCGATTTATACCACACCGACAGCGGCCATTTGGGG
TTACATCATTGGCTTTTTATGCTTATGGATTGTCTACTATCTGTTTAAGCTCATCACAGGCAAGGAAGGTATGGGTTATG
GTGACTTTAAGTTACTTGCTGCTTTAGGCGCATGGATGGGTCCTTTGCTACTGCCACTGATTGTGCTACTTTCTTCTTTC
GTAGGCGCCATTATCGGCATCATCCTGCTCAAAGTCCGTAAAGAAAATCAGCCTTTTGCTTTTGGCCCATATATTGCCAT
CGCAGGATGGATTGCCTTTTTGTGGGGTGAGCAAATTATGAAAATATATTTAGGCAATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A3S9APA7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	78.671	100	0.787
pilD	Acinetobacter baumannii D1279779	78.947	99.65	0.787
pilD	Vibrio cholerae strain A1552	52.985	93.706	0.497
pilD	Vibrio campbellii strain DS40M4	50.936	93.357	0.476
pilD	Neisseria gonorrhoeae MS11	45.055	95.455	0.43