Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEW87_RS01270 Genome accession   NZ_CP022188
Coordinates   284688..286400 (+) Length   570 a.a.
NCBI ID   WP_108971193.1    Uniprot ID   A0A2U8GWN1
Organism   Parazoarcus communis strain TSNA42     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279688..291400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW87_RS01250 (CEW87_01250) - 280159..280944 (-) 786 WP_108971189.1 EI24 domain-containing protein -
  CEW87_RS01255 (CEW87_01255) ffh 280955..282322 (-) 1368 WP_108971190.1 signal recognition particle protein -
  CEW87_RS01260 (CEW87_01260) - 282372..283202 (+) 831 WP_108971191.1 cytochrome C assembly family protein -
  CEW87_RS01265 (CEW87_01265) - 283356..284558 (+) 1203 WP_234421635.1 HlyC/CorC family transporter -
  CEW87_RS01270 (CEW87_01270) pilB 284688..286400 (+) 1713 WP_108971193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEW87_RS01275 (CEW87_01275) pilC 286426..287664 (+) 1239 WP_108971194.1 type II secretion system F family protein Machinery gene
  CEW87_RS01280 (CEW87_01280) pilD 287716..288567 (+) 852 WP_108971195.1 prepilin peptidase Machinery gene
  CEW87_RS01285 (CEW87_01285) - 288784..289065 (+) 282 WP_108971196.1 FmdB family zinc ribbon protein -
  CEW87_RS01290 (CEW87_01290) - 289090..289716 (+) 627 WP_108971197.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62271.47 Da        Isoelectric Point: 5.2301

>NTDB_id=237478 CEW87_RS01270 WP_108971193.1 284688..286400(+) (pilB) [Parazoarcus communis strain TSNA42]
MAANPQTALSGLARALIQNGRLTEADALSCSPTANGSSNAFLLEVCQRGLMNPREIARFAADTFGYPLLDINSVERGSLP
RDAIDRKLMNRHQVVALGKRQNRITLAVADPSNMRVLDEIRFQTGLQLDLIIVEVDKLGKLAEALSETAQQTLQEMTGED
FDMDLLQQDGPADQPADDSSEVDDAPVVKFIQKILIDAINEGASDVHFEPYEKYYRIRVRTDGVLREIAQPPLVLKEKIA
ARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSSLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQKKALLDAVER
PYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGEI
RDLETAEISVKAAQTGHLVLSTLHTNDAPSTLERLKNMGVAPFNIASSVIMITAQRLARRLCTCKKPMDIPIEALLQAGF
HEEDLDGSWQPYGPVGCERCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAAQAEREGVKDLRKSGLLKVRQGVTS
LEEVLASTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=237478 CEW87_RS01270 WP_108971193.1 284688..286400(+) (pilB) [Parazoarcus communis strain TSNA42]
ATGGCAGCAAACCCACAGACCGCATTGAGCGGACTTGCCCGGGCACTTATACAGAATGGTCGCCTCACCGAAGCCGACGC
GCTGAGCTGTTCACCCACTGCCAACGGTTCGTCCAACGCATTCCTGCTTGAGGTTTGCCAGCGCGGGCTGATGAATCCGC
GCGAAATCGCCAGATTCGCAGCCGATACGTTTGGTTACCCGCTGCTCGATATCAATTCGGTCGAGCGCGGCTCACTGCCG
CGCGACGCCATCGACCGCAAGCTGATGAACCGACACCAGGTCGTCGCGCTCGGCAAGCGACAGAACCGGATTACGCTTGC
GGTCGCCGACCCGTCCAACATGCGGGTGCTGGACGAGATTCGCTTTCAGACAGGGCTGCAACTTGACCTGATCATTGTCG
AGGTCGACAAACTCGGCAAACTCGCCGAGGCCCTCAGCGAAACGGCGCAACAAACGCTGCAGGAGATGACCGGCGAAGAC
TTCGACATGGACCTCCTGCAACAGGACGGTCCTGCCGATCAACCCGCAGACGACAGCTCGGAAGTCGATGACGCCCCTGT
CGTCAAGTTCATCCAGAAGATCCTGATCGACGCGATCAACGAGGGCGCTTCCGACGTTCATTTCGAACCCTACGAGAAGT
ACTACCGCATCCGCGTGCGTACCGACGGCGTGCTGCGCGAAATCGCGCAACCACCCCTGGTACTGAAGGAAAAGATCGCC
GCGCGGATCAAGGTCATTTCGCGCCTCGACATTTCAGAGAAGCGCGTGCCGCAGGATGGGCGGATGAAGCTGGTCCTCTC
CAAGAACAAGGCCATCGATTTCCGGGTGTCCTCGCTACCGACCCTGCATGGCGAGAAGATCGTGATGCGTATTCTCGACC
CGAGCTCGGCAATGCTCGGCGTCGATGCGCTCGGTTATGAACCCGAACAGAAGAAGGCACTTCTCGATGCCGTCGAACGC
CCCTACGGCATGATCCTGGTCACTGGACCAACCGGCTCGGGTAAGACGGTATCGCTCTACACCTGCCTCAACCTGCTCAA
CAAGGCGGGCGTCAACATTTCCACCGCCGAAGATCCAGCGGAAATCAACCTGCCGGGCATCAATCAGGTCAACGTGAACG
AAAAGGCGGGCCTGACGTTTGCTGCCTCGCTACGTGCCTTTCTGCGCCAGGATCCGGATGTCATCATGGTTGGCGAAATT
CGCGACCTCGAAACTGCCGAGATCTCGGTCAAGGCCGCGCAGACAGGCCACCTGGTCCTGTCCACGCTGCATACCAATGA
CGCACCCTCCACGCTGGAGCGCCTGAAGAACATGGGCGTGGCGCCATTCAACATTGCGTCGTCGGTGATCATGATCACCG
CACAGCGTCTCGCGCGCAGGCTGTGCACATGCAAGAAGCCGATGGACATTCCCATCGAAGCCCTCCTCCAGGCAGGATTT
CACGAAGAAGACCTGGACGGTAGCTGGCAACCCTACGGCCCGGTCGGCTGCGAGCGGTGCAAGGGCAGTGGCTACAAGGG
ACGTGTAGGCATCTATCAAGTCATGCCGGTTTCAGAGGAAATTGCGCATATCATCATGACCAACGGCAACTCGATGGATA
TTGCAGCCCAGGCAGAGCGCGAGGGCGTTAAGGATCTGCGCAAATCCGGTTTGCTCAAGGTTCGCCAAGGCGTGACCTCG
CTAGAGGAAGTGCTGGCAAGCACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-142)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GWN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.93

99.649

0.547
  pilB Acinetobacter baylyi ADP1

54.867

99.123

0.544
  pilB Legionella pneumophila strain ERS1305867

52.205

99.474

0.519
  pilF Neisseria gonorrhoeae MS11

52.043

98.772

0.514
  pilB Vibrio cholerae strain A1552

48.736

97.193

0.474
  pilB Vibrio campbellii strain DS40M4

46.631

98.947

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

46.702

98.421

0.46
  pilF Thermus thermophilus HB27

39.703

94.561

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.434

92.982

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

41.235

88.07

0.363

Multiple sequence alignment