Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEW83_RS10635 Genome accession   NZ_CP022187
Coordinates   2338518..2340230 (+) Length   570 a.a.
NCBI ID   WP_108949318.1    Uniprot ID   A0A2U8GPP6
Organism   Parazoarcus communis strain TSPY31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2333518..2345230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW83_RS10615 (CEW83_10605) - 2333954..2334763 (-) 810 WP_108949314.1 EI24 domain-containing protein -
  CEW83_RS10620 (CEW83_10610) ffh 2334775..2336142 (-) 1368 WP_108949315.1 signal recognition particle protein -
  CEW83_RS10625 (CEW83_10615) - 2336192..2337022 (+) 831 WP_108949316.1 cytochrome C assembly family protein -
  CEW83_RS10630 (CEW83_10620) - 2337185..2338378 (+) 1194 WP_234418772.1 HlyC/CorC family transporter -
  CEW83_RS10635 (CEW83_10625) pilB 2338518..2340230 (+) 1713 WP_108949318.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEW83_RS10640 (CEW83_10630) pilC 2340256..2341494 (+) 1239 WP_108949319.1 type II secretion system F family protein Machinery gene
  CEW83_RS10645 (CEW83_10635) pilD 2341546..2342397 (+) 852 WP_108949320.1 prepilin peptidase Machinery gene
  CEW83_RS10650 (CEW83_10640) - 2342611..2342892 (+) 282 WP_108949321.1 FmdB family zinc ribbon protein -
  CEW83_RS10655 (CEW83_10645) - 2342917..2343543 (+) 627 WP_108949322.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62254.46 Da        Isoelectric Point: 5.2254

>NTDB_id=237462 CEW83_RS10635 WP_108949318.1 2338518..2340230(+) (pilB) [Parazoarcus communis strain TSPY31]
MAANPQTALSGLARALIQNGRLTEADALSCSPTANGSSNAFLLEICQRGLMNPREIAKFAADTFGYPLLDINAVERGSLP
RDAIDRKLMNRHQVVALGKRQNRITLAVADPSNMRVLDEIRFQTGLQLDLIIVEVDKLGKLAEALSETAQQTLQDLTGED
FDMDLLQQDGPTDQPADDSSEVDDAPVVKFIQKILIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLVLKEKIA
ARIKVISRLDISEKRVPQDGRMKLVLSKTKAIDFRVSSLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQRKALLDAVER
PYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGEI
RDLETAEISVKAAQTGHLVLSTLHTNDAPSTLERLKNMGVAPFNIASSVIMITAQRLARRLCTCKKPMDIPIEALLQAGF
HEDDLDGSWQPYGPVGCERCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAAQAEREGVKDLRKSGLLKVRQGVTS
LEEVLASTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=237462 CEW83_RS10635 WP_108949318.1 2338518..2340230(+) (pilB) [Parazoarcus communis strain TSPY31]
ATGGCAGCAAACCCACAGACCGCACTGAGCGGACTTGCCCGGGCACTTATTCAGAATGGTCGCCTCACCGAAGCCGACGC
GCTGAGCTGTTCACCCACTGCAAACGGCTCGTCCAACGCCTTCCTGCTTGAGATCTGCCAACGCGGACTGATGAATCCGC
GCGAAATTGCAAAATTCGCAGCCGACACGTTTGGCTACCCCCTGCTGGACATCAATGCGGTCGAGCGCGGCTCGCTGCCC
CGCGATGCCATCGATCGCAAGCTGATGAACCGCCACCAGGTCGTCGCGCTCGGGAAACGCCAGAACCGGATTACGCTTGC
CGTCGCCGACCCGTCCAACATGCGGGTACTGGACGAAATCCGCTTTCAGACCGGGCTGCAGCTTGACCTGATCATTGTCG
AGGTCGACAAACTCGGCAAGCTCGCCGAAGCCCTTAGCGAGACTGCACAACAAACGCTGCAGGATTTGACCGGCGAAGAT
TTCGACATGGACCTCCTGCAACAGGACGGTCCTACCGATCAACCCGCAGACGACAGCTCGGAAGTCGATGACGCCCCCGT
CGTCAAGTTCATCCAGAAGATCCTGATCGACGCGATCAATGAAGGTGCGTCCGACATTCATTTCGAACCCTACGAGAAGT
ACTACCGCATTCGCGTTCGTACCGACGGCGTGCTACGTGAAATCGCACAACCACCCCTGGTGCTGAAGGAAAAGATCGCC
GCGCGGATCAAGGTCATTTCGCGCCTCGACATTTCGGAGAAGCGCGTACCGCAGGATGGCCGAATGAAGCTGGTGCTGTC
CAAGACCAAGGCCATTGATTTCCGCGTATCGTCTCTGCCGACCCTGCATGGCGAAAAAATCGTGATGCGTATTCTCGACC
CGAGTTCGGCAATGCTCGGTGTCGATGCGCTCGGCTACGAACCCGAGCAGAGGAAAGCGCTCCTCGACGCCGTTGAACGC
CCCTACGGCATGATTTTGGTCACCGGGCCGACCGGTTCGGGCAAGACGGTCTCGCTCTACACCTGTCTCAATCTGCTCAA
CAAGGCGGGCGTCAATATCTCCACCGCCGAGGATCCGGCGGAAATCAACCTGCCGGGCATCAATCAGGTCAACGTGAATG
AAAAAGCGGGCCTGACGTTTGCTGCGTCGCTACGCGCCTTTCTGCGCCAGGATCCGGACGTCATCATGGTCGGCGAGATT
CGCGACCTCGAAACTGCCGAAATTTCGGTCAAGGCCGCGCAGACGGGTCACCTCGTCCTGTCCACGCTGCATACCAATGA
TGCCCCCTCGACGCTCGAGCGCCTGAAGAACATGGGTGTGGCGCCATTCAACATCGCCTCGTCTGTGATCATGATCACCG
CACAGCGCCTTGCACGCCGGCTGTGCACCTGCAAGAAGCCAATGGACATTCCGATCGAAGCCCTGCTCCAGGCGGGCTTT
CACGAAGATGATCTGGACGGCAGCTGGCAACCCTACGGCCCGGTCGGCTGCGAGCGTTGCAAGGGCAGCGGCTACAAGGG
GCGGGTAGGCATCTACCAAGTCATGCCGGTCTCTGAGGAAATTGCGCATATCATCATGACCAACGGCAACTCGATGGATA
TCGCAGCCCAGGCTGAGCGTGAGGGCGTGAAGGATCTGCGCAAATCCGGTTTGCTCAAGGTTCGCCAGGGCGTGACCTCG
CTAGAGGAAGTGCTGGCAAGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-141)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GPP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.106

99.649

0.549
  pilB Acinetobacter baylyi ADP1

54.69

99.123

0.542
  pilB Legionella pneumophila strain ERS1305867

52.557

99.474

0.523
  pilF Neisseria gonorrhoeae MS11

51.865

98.772

0.512
  pilB Vibrio cholerae strain A1552

48.917

97.193

0.475
  pilB Vibrio campbellii strain DS40M4

46.235

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

46.466

99.298

0.461
  pilF Thermus thermophilus HB27

39.479

94.211

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

41.884

87.544

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.807

0.365

Multiple sequence alignment